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Peptidylarginine deiminase gene is differentially expressed in freshwater and brackish water rainbow trout

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Abstract

Peptidylarginine deiminase (PADI)-like cDNA sequence was isolated from rainbow trout (Oncorhynchus mykiss). It consists of a 111-bp 5′-untranslated region, a 731-bp 3′-UTR, and a 2,010-bp open reading frame encoding a protein of 669 amino acids. In the presence of calcium ions, PADI enzymes catalyze the post-translational modification reaction generating citrulline residues. Mammalian PADI enzymes are involved in a number of regulatory processes during cell differentiation and development such as skin keratinization, myelin maturation, and histone deimination. Though five PADI isotypes have been isolated from mammals, in bony fish only one PADI enzyme is present, which contains conserved amino acid residues responsible for catalysis and calcium ion-binding. Sequence identity of piscine PADI protein sequences available at gene databases exceeds 67%. Phylogenetic analyses revealed that not only piscine, but also amphibian and avian PADI-like proteins share most identical amino acid residues with mammalian PADI2. mRNA level of trout PADI-like gene is high in skin, fin, gills, brain, and spleen of rainbow trout. Quantitative Real-Time RT-PCR revealed that PADI gene is differentially expressed in liver, trunk kidney, and spleen of two trout strains, the freshwater-cultured STEELHEAD trout and the brackish water strain BORN.

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Acknowledgments

This work was funded by the Exzellenzförderprogramm Mecklenburg-Vorpommern (AU 08 026 entitled “DIREFO”). The authors wish to acknowledge B. F. Koop and W. Davidson for providing the cGRASP chip (http://web.uvic.ca/grasp/). We are grateful to J. Vogt, B. Schöpel, and I. Hennings for excellent technical assistance and to L. Speck and M. Fuchs for sequencing.

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Authors and Affiliations

  1. Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere (FBN), Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany

    Alexander Rebl, Klaus Wimmers & Tom Goldammer

  2. Friedrich-Loeffler-Institut (FLI), Am Südufer 10, 17493, Greifswald-Insel Riems, Germany

    Bernd Köllner

  3. Landesforschungsanstalt für Landwirtschaft und Fischerei Mecklenburg-Vorpommern (LFA-MV), Südstraße 10a, 18375, Born, Germany

    Eckhard Anders

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  1. Alexander Rebl
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  2. Bernd Köllner
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  3. Eckhard Anders
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  4. Klaus Wimmers
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  5. Tom Goldammer
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Correspondence to Alexander Rebl.

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Rebl, A., Köllner, B., Anders, E. et al. Peptidylarginine deiminase gene is differentially expressed in freshwater and brackish water rainbow trout. Mol Biol Rep 37, 2333–2339 (2010). https://doi.org/10.1007/s11033-009-9738-5

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  • DOI: https://doi.org/10.1007/s11033-009-9738-5

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