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Early low-temperature responsive mitogen activated protein kinases RaMPK1 and RaMPK2 from Rheum australe D. Don respond differentially to diverse stresses

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Abstract

Rheum grows luxuriantly in a niche of low temperature (LT) at high altitudes in Himalayan belt. The plant is expected to harbor novel genes particularly for tolerance to LT. Using differential display, two cDNAs RaMPK1 and RaMPK2, showing homology to mitogen-activated protein kinases (MAPKs) were isolated. As compared to RaMPK1, RaMPK2 exhibited strong up-regulation in response to LT. RaMPK1 was novel in terms of possessing a small glutamine and proline rich region at the N-terminal end. Secondly, though RaMPK1 showed homology with salicylic acid (SA) responsive MAPKs, the gene was down-regulated by SA but activated by jasmonate (JA). Abscisic acid (ABA) and polyethylene glycol (PEG) also down-regulated RaMPK1. RaMPK2 showed down-regulation within 5 min of exposure to JA and SA treatments, followed by gradual increase in expression. Expression of RaMPK2 was wavy in response to ABA and PEG treatment. Results are discussed in light of the novelty of these MAPKs.

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Acknowledgments

Authors thank the Department of Biotechnology (DBT), Government of India for funding the projects entitled “Bioprospecting genes for adaptive and growth strategies in cold desert plant species of Spiti” and “Novel products from Western Himalayas: antioxidant formulations, cloning of low temperature related cDNA, and development of microbial formulations”. Sanjay Ghawana acknowledges Council of Scientific and Industrial Research (CSIR) for awarding Senior Research Fellowship. Manuscript represents IHBT communication number 0776.

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Authors and Affiliations

  1. Biotechnology Division, Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research, P.O. Box 6, Palampur, HP, 176061, India

    Sanjay Ghawana, Sanjay Kumar & Paramvir Singh Ahuja

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  1. Sanjay Ghawana
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  2. Sanjay Kumar
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  3. Paramvir Singh Ahuja
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Correspondence to Sanjay Kumar.

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Ghawana, S., Kumar, S. & Ahuja, P.S. Early low-temperature responsive mitogen activated protein kinases RaMPK1 and RaMPK2 from Rheum australe D. Don respond differentially to diverse stresses. Mol Biol Rep 37, 933–938 (2010). https://doi.org/10.1007/s11033-009-9726-9

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  • DOI: https://doi.org/10.1007/s11033-009-9726-9

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