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Molecular characterization, expression profile and association analysis with carcass traits of porcine LCAT gene

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Abstract

The lecithin cholesterol acyltransferase gene (LCAT) plays an important role in lipoprotein metabolism, especially in the process termed ‘reverse cholesterol transport’. In this study, we obtained the 1,434 bp mRNA sequence of porcine LCAT including the full coding region and encoding a protein of 472 amino acids. The sequence was deposited into the GenBank under the accession no. EU717835. The genomic sequence of this gene which contains six exons and five introns, is 3,712 bp in length (GQ379050). Bioinformatic analysis of the 5′ regulatory region has revealed that some transcription factor Sp1, AP-1, AP-2 and NF-kappaB were represented in this region. Tissue expression analysis showed that the porcine LCAT gene is ubiquitously expressed in all examined tissues. Phylogenetic tree was constructed by aligning the amino acid sequences of different species. Moreover, we found a single nucleotide polymorphism (SNP, C/G266) in intron 1 of the LCAT gene and association analysis showed that it was significantly associated with ratio of lean to fat (P < 0.05), caul fat weight (P < 0.01), leaf fat weight (P < 0.05), carcass length (P < 0.05) and bone percentage (P < 0.05). Our study will lay the groundwork for the further investigations on the detailed physiological function of LCAT in pig models.

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Acknowledgments

This study was supported financially by the National “863” project of P. R. China (2007AA10Z166), National High Technology Development Project (2006BAD01A08-01), National “973” Program of P. R. China (2006CB102102), and Hubei Province Key Project of Science and Technology (2006AA202A01).

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Authors and Affiliations

  1. Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, 430070, Wuhan, People’s Republic of China

    Mu Qiao, Feng-E Li, Si-Wen Jiang, Yuan-Zhu Xiong & Chang-Yan Deng

  2. Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Hubei Academy of Agriculture Science, 430209, Wuhan, People’s Republic of China

    Hua-Yu Wu

  3. College of Animal Science, Huazhong Agricultural University, 430070, Wuhan, People’s Republic of China

    Chang-Yan Deng

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  1. Mu Qiao
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Correspondence to Chang-Yan Deng.

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Qiao, M., Wu, HY., Li, FE. et al. Molecular characterization, expression profile and association analysis with carcass traits of porcine LCAT gene. Mol Biol Rep 37, 2227–2234 (2010). https://doi.org/10.1007/s11033-009-9709-x

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  • DOI: https://doi.org/10.1007/s11033-009-9709-x

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