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Two novel SNPs in coding region of the caprine Fat-inducing transcript gene and their association with growth traits

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Abstract

Fat-inducing transcript (FIT) are endoplasmic reticulum resident membrane proteins that induce lipid droplet accumulation. It plays a crucial role in the fundamental process of fat storing. In this study, on the basis of PCR–SSCP and DNA sequencing methods, polymorphisms of the FIT gene were detected in 254 individuals from three goat breeds. The results showed that only P5 locus had two SNPs, resulting in a synonymous mutation (NM_001103095: m.422T > A/G resulting in S124S) and a missense mutation (NM_001103095: m.454 A > G resulting in Q135R). The associations between polymorphic loci and the growth traits of Boer and Xuhuai goat were analysed, and significant association were found in body height and chest circumference index (P < 0.05). Genotype BB of the Boer was associated with a higher chest circumference index (P < 0.05) than Genotype AC. In Xuhuai breed, Genotype BB was associated with a lower body height (P < 0.05) than Genotype AA. However, Genotype BB and AC had greater chest circumference index than Genotype AA (P < 0.01).

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References

  1. Martin S, Parton RG (2006) Lipid droplets: a unified view of a dynamic organelle. Nat Rev Mol Cell Biol 7:373–378

    Article  CAS  PubMed  Google Scholar 

  2. Brasaemle DL et al (2004) Proteomic analysis of proteins associated with lipid droplets of basal and lipolytically stimulated 3T3–L1 adipocytes. J Biol Chem 279:46835–46842

    Article  CAS  PubMed  Google Scholar 

  3. Liu P et al (2004) Chinese hamster ovary K2 cell lipid droplets appear to be metabolic organelles involved in membrane traffic. J Biol Chem 279:3787–3792

    Article  CAS  PubMed  Google Scholar 

  4. Beller M et al (2006) Characterization of the Drosophila lipid droplet subproteome. Mol Cell Proteomics 5:1082–1094

    Article  CAS  PubMed  Google Scholar 

  5. Chang BH, Chan L (2007) Regulation of triglyceride metabolism III Emerging role of lipid droplet protein ADFP in health and disease. Am J Physiol Gastrointest Liver Physiol 292:G1465–G1468

    Article  CAS  PubMed  Google Scholar 

  6. Mullner H, Daum G (2004) Dynamics of neutral lipid storage in yeast. Acta Biochim Pol 51:323–347

    PubMed  Google Scholar 

  7. Spiegelman BM, Flier JS (2001) Obesity and the regulation of energy balance. Cell 104:531–543

    Article  CAS  PubMed  Google Scholar 

  8. Kadereit B et al (2008) Evolutionarily conserved gene family important for fat storage. Proc Natl Acad Sci U S A 105:94–99

    Article  CAS  PubMed  Google Scholar 

  9. Luo J, Wang ZY (1998) Practical production technology of meat goat. Xi’an: The Publishing House of Shaanxi Science and Technology

  10. Mullenbach R, Lagoda PJ, Welter C (1989) An efficient saltchloroformextraction of DNA from blood and tissue. TrendsGenet 5:391

    CAS  Google Scholar 

  11. Yeh FC, Yang RC, Boyle TBJ, Ye ZH, Mao JX (1997) POPGENE, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Canada

    Google Scholar 

  12. Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331

    CAS  PubMed  Google Scholar 

  13. Bland JM, Altman DG (1995) Multiple significance tests: the Bonferroni method. BMJ 310:170

    CAS  PubMed  Google Scholar 

  14. Vaiman D, Mercier D, Moazami-Goudarzi K, Eggen A, Ciampolini R, Lepingle A, Velmala R, Kaukinen J, Varvio SL, Martin P, Levéziel H, Guérin G (1994) A set of 99 cattle microsatellites characterization synteny mapping and polymorphism. Mamm Genome 5:288–297

    Article  CAS  PubMed  Google Scholar 

  15. Evans MA, Shronts EP (1992) Intestinal fuels: glutamine, shortchain fatty acids, and dietary fiber. J Am Diet Assoc 92:1239–1249

    CAS  PubMed  Google Scholar 

  16. Schulman SP et al (2006) L-arginine therapy in acute myocardial infarction: the vascular interaction with age in myocardial infarction (VINTAGE MI) randomized clinical trial. JAMA 295:58–64

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by Natural Science Foundation of Jiangsu Province (No. BK2008120), the “13115” Sci-Tech innovation program of Shaanxi province (2008ZDKG-11), Research Fund for the Doctor Program of Higher Education of China (No. 20080712001). Talents Foundation of Northwest A&F University (No.01140411), the National “863” Program of the People’s Republic of China (No. 2008AA10Z138).

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Authors and Affiliations

  1. Institute of Cellular and Molecular Biology, College of Life Science, Xuzhou Normal University, 221116, Xuzhou, Jiangsu, People’s Republic of China

    Jiajie Sun, Chunlei Zhang, Hong Chen, Xingtang Fang, Qijiang Jin, Danxia Chen, Xiuying Shi & Yu Du

  2. Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, Shaanxi, China

    Hong Chen

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  1. Jiajie Sun
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  2. Chunlei Zhang
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  3. Hong Chen
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  4. Xingtang Fang
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  5. Qijiang Jin
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  7. Xiuying Shi
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  8. Yu Du
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Correspondence to Hong Chen.

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Sun, J., Zhang, C., Chen, H. et al. Two novel SNPs in coding region of the caprine Fat-inducing transcript gene and their association with growth traits. Mol Biol Rep 37, 485–490 (2010). https://doi.org/10.1007/s11033-009-9672-6

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  • DOI: https://doi.org/10.1007/s11033-009-9672-6

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