Change of season-specific telomere lengths in Ginkgo biloba L.
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Telomeres have lately received considerable attention in the development of tree species. Normal somatic cells have limited replicative capacity and telomeres get shorten with each round of DNA replication. For broad-leaved tree species, to determine what changes happen to their somatic cells in its annual development cycle, an exhaustive research on different ages of gingko trees telomere length changes was carried out. Analysis of changes in leaf telomere lengths in the annual development cycle of Ginkgo biloba L. showed no significant changes (P > 0.05) from April to August, but a dramatic decrease in September and October (P < 0.05). Statistical analyses showed that TRF length of males and females are equal, the p values of the three age groups comparison were all bigger than 0.05. The results showed that specific apoptotic changes occur in the annual development cycle of Ginkgo biloba L.
KeywordsAge Annual development cycle Gender Ginkgo biloba L. TRF length
This work was supported by the National Natural Science Foundation of China (Grant No. 30571473). The authors thank Nan Qiao and members of the Botany laboratory for their support during data analysis and many helpful discussions and Renyuan Zhu and Xiaohong Zhou for support during sample collection in the Wofo Temple of the Beijing Botanical Garden and the Fragrant Hills Park. The authors also thank Pengjun Cheng for revising the manuscript.
- 12.Nakamura K, Izumiyama-Shimomura N, Sawabe M, Arai T, Aoyagi Y, Fujiwara M, Tsuchiya E, Kobayashi Y, Kato M, Oshimura M, Sasajima K, Nakachi K, Takubo K (2002) Comparative analysis of telomere lengths and erosion with age in human epidermis and lingual epithelium. J Invest Dermatol 119:1014–1019CrossRefPubMedGoogle Scholar
- 13.Grant JD, Broccoli D, Muquit M, Manion FJ, Tisdall J, Ochs MF (2001) Telometric: a tool providing simplified, reproducible measurements of telomeric DNA from constant field agarose gels. Biotechniques 31:1314–1316, 1318Google Scholar