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Effects of ovariectomy on microsatellite instability in rat colon tumors induced by 1,2-dimethylhydrazine

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Abstract

To study the effects of ovariectomy on tumorigenesis and microsatellite instability (MSI) in rat colon tumors induced by 1,2-dimethylhydrazine, to elucidate the association between postmenopausal ovarian hormones depletion and MSI pathway in colorectal tumorigenesis. Forty female Wistar rats were randomly divided into two groups: Ovariectomized (Ovx) group and Sham-ovariectomized (Sham-Ovx) group. All rats were injected intraperitoneally with 1,2-dimethylhydrazine (DMH) (20 mg/kg b.w) once a week for 20 weeks. Ten weeks after the final DMH injection, all the rats were sacrificed to collect tumors. Microsatellite instability of six microsatellite loci was detected using fluorescent PCR followed by fragment analysis on automatic DNA sequencer with GeneScan 3.7 software. The tumor multiplicity in the OVX group was significantly higher than that in the Sham-OVX group (3.6 ± 1.4 vs. 2.4 ± 1.6, P < 0.05). The incidence of MSI-positive tumors in OVX group was higher than that in Sham-OVX group (32.1 vs. 10.8%, P < 0.05).The incidence of tumors showing MSI at multiple loci in OVX group was also higher than that in Sham-OVX group (18.9 vs. 2.7%, P < 0.05). Ovariectomy increased tumor formation and the frequency of MSI in DMH-induced colon tumors. It implied that postmenopausal ovarian hormones depletion might influence colorectal tumorigenesis through MSI pathway.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 30500488).

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Authors and Affiliations

  1. Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, 430022, Wuhan, Hubei Province, China

    Fei Xu, Guobin Wang, Kailin Cai, Ronglin Zhai & Shouyuan Tang

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  1. Fei Xu
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  2. Guobin Wang
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  3. Kailin Cai
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  4. Ronglin Zhai
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  5. Shouyuan Tang
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Correspondence to Kailin Cai.

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Fei Xu and Guobin Wang contributed equally to this work.

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Xu, F., Wang, G., Cai, K. et al. Effects of ovariectomy on microsatellite instability in rat colon tumors induced by 1,2-dimethylhydrazine. Mol Biol Rep 37, 1397–1401 (2010). https://doi.org/10.1007/s11033-009-9523-5

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  • DOI: https://doi.org/10.1007/s11033-009-9523-5

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