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Phenotypic and molecular characterization of two novel CTX-M enzymes carried by Klebsiella pneumoniae

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Abstract

Two clinical strains (Klebsiella pneumoniae 516 and K. pneumoniae 1335) collected in September 2006 from different hospitals in Anhui Province (China) harboured two novel plasmid-mediated bla CTX-M genes, designated bla CTX-M-80 and bla CTX-M-81, respectively. Both CTX-M-80 with pI of 9.0 and CTX-M-81 with pI of 8.4 were extended-spectrum β-lactamases (ESBLs). The results of susceptibility testing demonstrated two enzymes were highly activity against broad spectrum β-lactams, but the level of resistance was reduced with the addition of β-lactamase inhibitors. The bla CTX-M-80 gene was detected on a 110-kb plasmid and the bla CTX-M-81 gene existed on a 120-kb plasmid. The deduced amino acid sequence of CTX-M-80 differed from that of CTX-M-3 by the substitution Ala-27→Val, and CTX-M-81 possessed the Lys→Glu, Lys→Gln, and Asn→His changes at respective position 82, 98, and 132 in compassion with CTX-M-14. The enzymatic properties showed CTX-M-80 and CTX-M-81 had higher affinities for penicillin G (lower Km values) than for cephalosporins. The activities of novel enzymes against ceftazidime were undetectable or limited, as indicated by MICs data, the same response being observed for many other CTX-M enzymes. This report was evidence of the diversity of CTX-M-type ESBLs in China.

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Acknowlegments

This study was supported by National Natural Science Foundation of China (No. 30571654).

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Authors and Affiliations

  1. Department of Infectious Diseases, First Affiliated Hospital of Anhui Medical University, 230022, Hefei, Anhui, China

    Jun Cheng, Wei Gao, Jun Yin, Zhen Sun, Ying Ye, Yu-Feng Gao, Xu Li & Jia-Bin Li

  2. Anhui Center for Surveillance of Bacterial Resistance, 230022, Hefei, Anhui, China

    Ying Ye, Xu Li & Jia-Bin Li

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  1. Jun Cheng
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  2. Wei Gao
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  3. Jun Yin
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  4. Zhen Sun
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  5. Ying Ye
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  6. Yu-Feng Gao
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  7. Xu Li
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  8. Jia-Bin Li
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Correspondence to Xu Li or Jia-Bin Li.

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Jun Cheng and Wei Gao contributed equally to this work.

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Cheng, J., Gao, W., Yin, J. et al. Phenotypic and molecular characterization of two novel CTX-M enzymes carried by Klebsiella pneumoniae . Mol Biol Rep 37, 1261–1267 (2010). https://doi.org/10.1007/s11033-009-9499-1

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  • DOI: https://doi.org/10.1007/s11033-009-9499-1

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