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ThPOD3, a truncated polypeptide from Tamarix hispida, conferred drought tolerance in Escherichia coli

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Abstract

The ThPOD1 gene encodes a peroxidase and was isolated from a Tamarix hispida NaCl-stress root cDNA library. We found that ThPOD1 expression could be induced by abiotic stresses such as cold, salt, drought and exogenous abscisic acid. These findings suggested that ThPOD1 might be involved in the plant response to environmental stresses and ABA treatment. To elucidate the function of this gene, recombinant plasmids expressing full-length ThPOD1 as well as ThPOD2 (aa 41-337), and ThPOD3 (aa 73-337) truncated polypeptides were constructed. SDS–PAGE and Western blot analyses of the fusion proteins revealed that the molecular weights of ThPOD1, ThPOD2 and ThPOD3 were ~57, ~50 and ~47 kDa, respectively. Stress assays of E. coli treated with the recombinant plasmids indicated that ThPOD3 could improve resistance to drought stress. This finding could potentially be used to improve plant tolerance to drought stress via gene transfer.

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Acknowledgments

This study was supported by national natural science foundation (Grant No. 30571509), Heilongjiang province scientific and technological project (Grant No. GB06B303 and WB07N02).

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Authors and Affiliations

  1. Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Ministry of Education, Northeast Forestry University, 150040, Harbin, China

    Xiao-Hong Guo, Jing Jiang, Bai-Chen Wang, Hui-Yu Li, Yu-Cheng Wang, Chuan-Ping Yang & Gui-Feng Liu

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  1. Xiao-Hong Guo
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Correspondence to Gui-Feng Liu.

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Guo, XH., Jiang, J., Wang, BC. et al. ThPOD3, a truncated polypeptide from Tamarix hispida, conferred drought tolerance in Escherichia coli . Mol Biol Rep 37, 1183–1190 (2010). https://doi.org/10.1007/s11033-009-9484-8

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