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The prevalence of factor V Leiden, prothrombin G20210A and methylenetetrahydrofolate reductase polymorphism C677T among G6PD deficient individuals from Western Iran

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Abstract

It has been suggested that the allele frequency of thrombophilic mutations is affected by glucose-6-phosphate dehydrogenase (G6PD) deficiency. The prevalence of thrombophilic mutations were studied in sixty G6PD deficient individuals including 57 males and three females with the mean age of 15 ± 3.08 and 110 age and sex matched healthy individuals consisted of 95 males and 15 females with the mean age of 16.19 ± 2.17 from the Kermanshah Province of Iran. Using a combination of PCR-RFLP technique, single strand conformation polymorphism (SSCP) analysis and DNA sequencing polymorphic G6PD mutations were identified. The factor V Leiden, prothrombin G20210A and methylenetetrahydrofolate reductase (MTHFR) C677T were detected by PCR-RFLP method using MnlI, HindIII and HinfI restriction enzymes, respectively. Three mutations, G6PD Mediterranean, G6PD Chatham and G6PD Cosenza were identified in 60 G6PD deficient individuals with highest prevalence of G6PD Mediterranean (91.6%). In G6PD deficient individuals the prevalence of factor V Leiden tended to be higher (5%) compared to healthy individuals (2.7%). The prevalence of prothrombin G20210A mutation in G6PD deficient individuals was 1.7%. However, in normal subjects the prevalence of this mutation was 2.7%. The frequency of T allele in G6PD deficient individuals were insignificantly higher (29.16%) than those in healthy individuals (26.8%). Our finding indicates that the prevalence of factor V Leiden, prothrombin G20210A and MTHFR C677T in G6PD deficient individuals is not statistically different compared to normal subjects and G6PD deficiency is not associated with these thrombophilic mutations in Western Iran.

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Acknowledgments

This work was financially supported by a grant from Kermanshah University of Medical Sciences, Kermanshah, Iran.

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Authors and Affiliations

  1. Faculty Member of Medical Biology Research Center, Medical School, Kermanshah University of Medical Sciences, P.O. Box 67155-1957, Daneshgah Avenue, Kermanshah, Iran

    Hadi Mozafari, Zohreh Rahimi, Azadeh Heidarpour & Mahsa Fallahi

  2. Department of Biochemistry, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Zohreh Rahimi

  3. Department of Biochemistry, Medical School, Kurdistan University of Medical Sciences, Kurdistan, Iran

    Zohreh Rahimi

  4. Department of Medicine, Division of Hematology, Albert Einstein College of Medicine, Bronx, NY, USA

    Adraiana Muniz

  5. Department of Physiology and Biophysics, Division of Hematology, Albert Einstein College of Medicine, Bronx, NY, USA

    Adraiana Muniz

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  1. Hadi Mozafari
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  2. Zohreh Rahimi
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  4. Mahsa Fallahi
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Correspondence to Hadi Mozafari.

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Mozafari, H., Rahimi, Z., Heidarpour, A. et al. The prevalence of factor V Leiden, prothrombin G20210A and methylenetetrahydrofolate reductase polymorphism C677T among G6PD deficient individuals from Western Iran. Mol Biol Rep 36, 2361–2364 (2009). https://doi.org/10.1007/s11033-009-9458-x

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  • DOI: https://doi.org/10.1007/s11033-009-9458-x

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