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Cloning and characterization of full-length coding sequence (CDS) of the ovine 37/67-kDa laminin receptor (RPSA)

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Abstract

The 37-kDa Laminin Receptor Precursor (LRP)/67-kDa Laminin Receptor (LR), also known as ribosomal protein SA (RPSA), had been identified as a putative cell surface receptor for prions. Herein, we isolated the full-length coding sequence (CDS) of the ovine 37/67-kDa LRP/LR gene and submitted it to the GenBank under accession number EF649775. The open reading frame (ORF) of the 37/67-kDa LRP/LR CDS is 885 bp in length, containing six exons encoding a protein of 295 amino acids. The nucleotide sequence presented here is well coincided with the whole ovine genome of the 37/67-kDa LRP/LR previously published. Moreover, we identified four novel single nucleotide polymorphism sites (SNPs) at position 324 in exon 4, positions at 809, 875, and 881 in exon 7, respectively. Further, based on the deduced amino acid sequence alignment of the 37/67-kDa LRP/LR from human, cattle, mice, pig, chicken, and sheep, we also identified three polymorphic amino acid sites (PAAs) at residues 241, 272, and a novel site at residue 270 in the putative indirect prion protein (PrP) interaction region (180–285) on 37/67-kDa LRP/LR. Prediction of protein secondary structure further indicated that PAAs at residues 241, 270 and 272 may cause protein conformation changes as predicted, which may affect on the binding with prion protein. In addition, multiple-tissues RT-PCR results revealed that 37/67-kDa LRP/LR mRNA is expressed in all the 11 selected ovine tissues.

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Acknowledgments

We thank Dr. Pengju Guo (CSIRO Livestock Industries, Australia) for help with the research. This work was supported by the Natural Science Foundation of China (Project No. 30571399 and No. 30771622), the 973 Project (No. 2005CB523000) and the Key Projects of National Science & Technology Pillar Program in the 11th 5 years of China (Program No. 2006BAD06A13).

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Authors and Affiliations

  1. National Animal TSE Laboratory, College of Veterinary Medicine, China Agriculture University, Yuanmingyuan West Road 2, 100193, Beijing, People’s Republic of China

    Junwen Qiao, Xiaoou Su, Yiqin Wang, Jianmin Yang, Mohammed Kouadir, Xiangmei Zhou, Xiaomin Yin & Deming Zhao

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  1. Junwen Qiao
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Correspondence to Deming Zhao.

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Qiao, J., Su, X., Wang, Y. et al. Cloning and characterization of full-length coding sequence (CDS) of the ovine 37/67-kDa laminin receptor (RPSA). Mol Biol Rep 36, 2131–2137 (2009). https://doi.org/10.1007/s11033-008-9426-x

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