Abstract
Insulin receptor substrate-1(IRS-1) gene is one member of the Insulin receptor substrate (IRS) gene family, which plays an important role in mediating the growth of skeletal muscle and the molecular metabolism of type 2 diabetes. Here, we cloned a 3,573 bp fragment of the partial CDS sequence of porcine IRS-1 gene by in silicon cloning strategy and RT-PCR method. The porcine IRS-1 gene was assigned to SSC15q25 by using IMpRH. Sequencing of PCR products from Duroc and Tibetan pig breeds identified one SNP in exon 1 of porcine IRS-1 gene (C3257A polymorphisms). Association analysis of genotypes with the growth traits, anatomy traits, meat quality traits and physiological biochemical indexes traits showed that different genotypes at locus 3,257 of IRS-1 have significant differences in carcass straight length in pigs (P = 0.0102 < 0.05).
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Acknowledgments
We thank Dr Martine Yerle of INRA, France for providing the RH panel. We thank Dr Wu Xiaoxiong for technical help. The project was supported by the Key Project of the National Basic Research and Developmental Plan of China (2006CB102105), the National Natural Science Foundation of China (30571328), and National High Science and Technology Foundation of China “863” (2006AA10Z195).
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Niu, PX., Huang, Z., Li, CC. et al. Cloning, chromosomal localization, SNP detection and association analysis of the porcine IRS-1 gene. Mol Biol Rep 36, 2087–2092 (2009). https://doi.org/10.1007/s11033-008-9420-3
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DOI: https://doi.org/10.1007/s11033-008-9420-3