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Characterization of the porcine differentially expressed PDK4 gene and association with meat quality

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Abstract

To investigate the differential expression of genes in the skeletal muscle between Yorkshire and Chinese indigenous breed Meishan pigs, suppression subtractive hybridization was carried out and many genes were proved to be expressed significantly different in the two breeds. One gene highly expressed in Meishan but lowly expressed in Yorkshire specific library, shared strong homology with human pyruvate dehydrogenase kinase 4 (PDK4). Using semi-quantity and quantity PCR, We confirmed its differential expression between the two breeds. Temporal and spatial expression analysis indicated that porcine PDK4 gene is highly expressed in skeletal muscle and the highest in neonatal pigs. Complete cDNA cloning and sequence analysis revealed that porcine PDK4 gene contains an open reading frame of 1,221 bp. The deduced amino acid sequence showed conservation in evolution. A G/A mutation in intron 9 was identified and association analysis showed that it was significantly associated with intramuscular fat, muscle water content.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (30400313), the National Key Foundation Research and Development Program of China (2006CB102102) and the National High Technology Research and Development Program of China (2006AA10Z1D6).

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Correspondence to Ming-Gang Lei or Yuan-Zhu Xiong.

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Lan, J., Lei, MG., Zhang, YB. et al. Characterization of the porcine differentially expressed PDK4 gene and association with meat quality. Mol Biol Rep 36, 2003–2010 (2009). https://doi.org/10.1007/s11033-008-9411-4

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