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Mutational analysis of the -10 region from the Mycobacterium tuberculosis lipF promoter

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Abstract

The promoter driving expression of the Mycobacterium tuberculosis gene lipF (Rv3487c) had previously been identified as being upregulated by acidic stress. Subsequently a 59 base pair (bp) acid inducible minimal promoter region was isolated in which a putative -10 region was identified. In this study we use mutational analysis to investigate the -10 region of the lipF promoter. Mutations within this region lead to a dramatic decrease of promoter activity, while a mutation outside of this region does not affect promoter activity.

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Acknowledgements

This work was funded by an American Lung Association Grant, a California Lung Association grant, and a Potts Memorial Foundation grant.

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Authors and Affiliations

  1. Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA

    Michelle Gonzales

  2. Basic Medical Sciences, College of Osteopathic Medicine, Western University of Health Sciences, 309 E. Second St., Pomona, CA, 91766, USA

    Beatrice Saviola

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  1. Michelle Gonzales
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  2. Beatrice Saviola
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Correspondence to Beatrice Saviola.

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Gonzales, M., Saviola, B. Mutational analysis of the -10 region from the Mycobacterium tuberculosis lipF promoter. Mol Biol Rep 36, 1225–1229 (2009). https://doi.org/10.1007/s11033-008-9301-9

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  • DOI: https://doi.org/10.1007/s11033-008-9301-9

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