Skip to main content
SpringerLink
Log in

Protective effects of N-acetylcysteine in isoproterenol-induced myocardium injury in rats

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Isoproterenol (ISO) has been found to cause severe injury in the myocardium. The aim of this study was to investigate the protective effects of N-acetylcysteine (NAC) on ISO-induced myocardial injury in rats and its underlying mechanisms. Fouty male Wistar rats were randomly divided into four groups: control, ISO, NAC, and ISO + NAC group. Myocardial histopathological observation were performed; The activities of creatine kinase isoenzyme-MB (CK-MB) and lactate dehydrogenase (LDH) were examined; Myocardium TNF-αand IL-1β gene expressions were examined by RT-PCR analysis; Myocardial expressions of TNF-αand IL-1βproteins were observed by immunohistochemical assay and western blotting analysis. The myocardial injury induced by ISO was significantly reduced by the treatment of NAC as judged by the reduction of myocardial necrosis. Compared with ISO group, rats pre-injected with NAC showed a significant decrease in the activities of cardiac marker enzymes such as CK-MB and LDH in serum. NAC inhibits the pro-inflammatory factors expressions (TNF-αand IL-1β) stimulated by ISO. In conclusion, NAC exerts significant cardio-protective effects against ISO-induced myocardial injury in rats, likely regulating pro-inflammatory factors expressions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Yeager JC, Iams SG (1982) The hemodynamics of isoproterenol-induced cardiac failure in the rat. Life Sci 30:299–306

    Article  PubMed  CAS  Google Scholar 

  2. Hsu CC, Huang CN, Hung YC, Yin MC (2004) Five cysteinecontaining compounds have antioxidative activity in Balb/cA mice. J Nutr 134:149–152

    PubMed  CAS  Google Scholar 

  3. Demirkol O, Adams C, Ercal N (2004) Biologically important thiols in various vegetables and fruits. J Agric Food Chem 52:8151–8154

    Article  PubMed  CAS  Google Scholar 

  4. Wang X, Martindale JL, Holbrook NJ (2000) Requirement for ERK activation in cisplatin-induced apoptosis. J Biol Chem 275:39435–39443

    Article  PubMed  CAS  Google Scholar 

  5. Oh SH, Lima SC (2006) A rapid and transient ROS generation by cadmium triggers apoptosis via caspase-dependent pathway in HepG2 cells and this is inhibited through N-acetylcysteine-mediated catalase upregulation. Toxicol Appl Pharmacol 212:212–223

    Article  PubMed  CAS  Google Scholar 

  6. Carageorgiou H, Tzotzes V, Pantos C, Mourouzis C, Zarros A, Tsakiris S (2004) In vivo and in vitro effects of cadmium on adult rat brain total antioxidant status, acetylcholinesterase, (Na+,K+)-ATPase and Mg2+ -ATPase activities: protection by L-Cysteine. Basic Clin Pharmacol Toxicol 94:112–118

    Article  PubMed  CAS  Google Scholar 

  7. Park JE, Yang JH, Yoon SJ, Lee JH, Yang ES, Park JW (2002) Lipid peroxidation-mediated cytotoxicity and DNA damage in U937 cells. Biochimie 84:1199–1205

    Article  PubMed  CAS  Google Scholar 

  8. Reliene R, Fischer E, Schiestl RH (2004) Effect of N-acetylcysteine on oxidative DNA damage and the frequency of DNA deletions in atm-deficient mice. Cancer Res 64:5148–5153

    Article  PubMed  CAS  Google Scholar 

  9. Ahmed KK, Rana AC, Dixit VK (2004) Effect of Calotropis procera latex on isoproterenol induced myocardial infarction in albino rats. Phytomedicine 11:327–330

    Article  PubMed  Google Scholar 

  10. Plaa GL, Zimmerson HJ (1997) Evaluation of hepatotoxicity: physiological and biochemical measures of hepatic function. In: Mc Cuskey RS, Earnest DL (eds) Comprehensive toxicology. Cambridge University Press, Cambridge, pp 97–109

    Google Scholar 

  11. Fan H, Williams DL, Zingarelli B (2007) Differential regulation of lipopolysaccharide and Gram-positive bacteria induced cytokine and chemokine production in macrophages by Galpha(i) proteins. Immunology 122:116–123

    Article  PubMed  CAS  Google Scholar 

  12. Pagani FD, Baker LS, Hsi C (1992) Left ventricular systolic and diastolic dysfunction after infusion of tumor necrosis factor-alpha in conscious dogs. J Clin Invest 90:389–398

    Article  PubMed  CAS  Google Scholar 

  13. Ono K, Matsumori A, Shioi T (1998) Cytokine gene expression after myocardial infarction in rat hearts. Possible implication in left ventricular remodeling. Circulation 98:149–156

    PubMed  CAS  Google Scholar 

  14. Turner NA, Mughal RS, Warburton P (2007) Mechanism of TNFalpha-induced IL-1alpha, IL-1beta and IL-6 expression in human cardiac fibroblasts: effects of statins and thiazolidinediones. Cardiovasc Res 76:81–90

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Cardiovascular Department, First Clinical College of Harbin Medical University, 23 Youzheng Street, Harbin, 150001, People’s Republic of China

    Bin Liu, Weimin Li & Yue Li

  2. Department of Cardiology, Yangzhou Hongquan Hospital, Yangzhou, 225200, People’s Republic of China

    Zhonghua Wang

  3. Department of Cardiology, Shanghai Jiaotong University Affiliated First people’s Hospital, 85 Wujin Road, Shanghai, 200080, People’s Republic of China

    Hongli Li

  4. Department of Immunology, Harbin Medical University, Harbin, 150081, China

    Ping Liu

  5. International Medical Care Department, First Clinical College of Harbin Medical University, Harbin, 150001, People’s Republic of China

    Jun Fu

Authors
  1. Bin Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Weimin Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yue Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhonghua Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hongli Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ping Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jun Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jun Fu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liu, B., Li, W., Li, Y. et al. Protective effects of N-acetylcysteine in isoproterenol-induced myocardium injury in rats. Mol Biol Rep 36, 761–765 (2009). https://doi.org/10.1007/s11033-008-9241-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-008-9241-4

Keywords

Search

Navigation