Abstract
Since mutations on POU1F1 gene possibly resulted in deficiency of GH, PRL, TSH and POU1F1, this study revealed the polymorphism of goat POU1F1-AluI locus and analyzed the distribution of alleles on 13 indigenous Chinese goat breeds. The PCR-RFLP analysis showed the predominance of TT genotype and the frequencies of allele T varied from 0.757 to 0.976 in the analyzed populations (SBWC, Bo, XH and HM). Further study, distributions of genotypic and allelic frequencies at this locus were found to be significantly different among populations based on a χ2-test (P < 0.001), suggesting that the breed factor significantly affected the molecular genetic character of POU1F1 gene. The genetic diversity analysis revealed that Chinese indigenous populations had a wide spectrum of genetic diversity in goat POU1F1-AluI locus. However, the ANOVA analysis revealed no significant differences for gene homozygosty, gene heterozygosty, effective allele numbers and PIC (polymorphism information content) among meat, dairy and cashmere utility types (P > 0.05), suggesting that goat utility types had no significant effect on the spectrum of genetic diversity.
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Acknowledgements
This work was funded by Talents Foundation of Northwest A&F University (Doctoral program of NWSUAF, X. Y. Lan), the National “863” Program of China (No. 2008AA10Z138), Chinese National Natural Science Foundation (No. 30771544) and the Outstanding Talents Foundation of Northwest A&F University (No. 01140101).
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X. Y. Lan and M. J. Li equally contributed to this work.
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Lan, X.Y., Li, M.J., Chen, H. et al. Analysis of caprine pituitary specific transcription factor-1 gene polymorphism in indigenous Chinese goats. Mol Biol Rep 36, 705–709 (2009). https://doi.org/10.1007/s11033-008-9232-5
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DOI: https://doi.org/10.1007/s11033-008-9232-5