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Identification of a novel human lactate dehydrogenase gene LDHAL6A, which activates transcriptional activities of AP1(PMA)

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Abstract

l-lactate dehydrogenase is a crucial enzyme in the process of glycolysis. Here we report the cloning and characterization of another novel lactate dehydrogenase gene, named as LDHAL6A (lactate dehydrogenase A-like 6A), which encodes a 332-amino-acid protein. The LDHAL6A gene consists of seven exons, and is mapped to 11p15.1 by searching the UCSC genomic database. By RT-PCR analysis in various tissues, LDHAL6A was found to be exclusively expressed in human testis. Subcellular localization demonstrated that LDHAL6A protein was located in the cytoplasm when overexpressed in COS7 cells. Furthermore, we found that the recombinant protein GST-LDHAL6A can catalyze the pyruvate convert into the lactate with NADH as its coenzyme. And in the dual luciferase reporter system, expression of LDHAL6A was able to activate transcriptional activities of AP1(PMA).

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Abbreviations

LDH:

l-lactate dehydrogenase

LDHAL6A:

Lactate dehydrogenase A-like 6A

NAD+:

Nicotinamide adenine dinucleotide

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Acknowledgements

This work was supported by the National 973 program of China, 863 projects of China and the National Natural Science foundation of China (30024001).

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Authors and Affiliations

  1. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 220 Handon Road, Shanghai, 200433, P.R. China

    Xinya Chen, Xiuting Gu, Yuxi Shan, Wenwen Tang, Jian Yuan, Zhaomin Zhong, Yingli Wang, Weixue Huang, Bo Wan & Long Yu

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  1. Xinya Chen
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  2. Xiuting Gu
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  3. Yuxi Shan
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  4. Wenwen Tang
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  7. Yingli Wang
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  9. Bo Wan
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  10. Long Yu
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Correspondence to Long Yu.

Additional information

Sequence date from this article has been deposited with the GenBank Data Library under Accession No. NM144972.

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11033_2008_9227_MOESM1_ESM.tif

LDHAL6A was located in the cytoplasm and might be on mitochondria. (a–c) COS7 cells were transiently transfected with pCMV-Myc-LDHAL6A. (a) Cells expressing Myc-tagged LDHAL6A were stained with monoclonal antibody against Myc epitope (green). (b) The cells transfected with pCMV-Myc-LDHAL6A was stained using mitotracker (red) as cell mitochondria localization marker. (c) The nuclei of the cells was stained with DAPI. (d) The superimposed image of (a), (b) and (c) beach pollutant (TIF 1267 kb)

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Chen, X., Gu, X., Shan, Y. et al. Identification of a novel human lactate dehydrogenase gene LDHAL6A, which activates transcriptional activities of AP1(PMA). Mol Biol Rep 36, 669–676 (2009). https://doi.org/10.1007/s11033-008-9227-2

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  • DOI: https://doi.org/10.1007/s11033-008-9227-2

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