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Frequency of cystathionine β-synthase 844INS68 polymorphism in Southern Iran

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Abstract

Iranian population with an Indo-European origin is one of the oldest populations in the world. Historical evidence suggests the close similarity in the origin of Iranian, European and north Indian population. However, there are few anthropological and genetic evidences on this subject. This study, which is the first report from Iran, was performed to investigate the genetic origin of Iranian population using a polymorphism in Cystathionine beta synthase (CBS) gene known as 844INS68bp in this respect, genomic DNA was extracted from the whole blood of 480 healthy normal blood donors referred to Fars Blood Transfusion Center, using a salting out method. The fragment containing 844INS68bp was amplified, the normal fragment was 174 bp and the fragment containing the insertion was 242 bp in length. Results indicated that 418 (87.08%) out of 480 individuals had a normal (N/N) genotype, 59 (12.29%) individuals were heterozygote (N/I) and 3 (0.63%) had homozygote a mutated genotype (I/I). The total frequency of 844INS68bp allele was found 6.8% which is similar to with the reported in White Caucasians. Comparison of the genotype of this study with the polymorphism in other populations revealed that Southern Iranian population has a great similarity with other Caucasians populations’ especially South Italy and North America while differed from East Asian and African populations. These results are in agreement with the result of other studied polymorphisms. Therefore, despite the great admixture of Iranian population with the neighboring non-Caucasian populations during the time, Iranian population still share a genetic background with other Caucasian populations.

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Acknowledgements

The authors would like to thank the Office of Vice Chancellor for Research of Shiraz University of Medical Sciences for financial support of this study and Dr Davood Mehrabani at Center for Development of Clinical Research of Nemazee Hospital for editorial assistance.

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Authors and Affiliations

  1. Human Genetics Research Group, Iranian Academic Center for Education & Research (ACECR), Fars Branch, Allay 43, Zand Street, Shiraz, Iran

    Sara Senemar & Masood Bazrgar

  2. Institute for Cancer Research, Medical School, Shiraz University of Medical Science, Shiraz, Iran

    Mehrnoosh Doroudchi, Abdul Mohammad Pezeshki & Abbas Ghaderi

  3. Blood Transfusion Center, Shiraz, Iran

    Ardeshir Torab-Jahromi

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  1. Sara Senemar
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  2. Mehrnoosh Doroudchi
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  3. Abdul Mohammad Pezeshki
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  4. Masood Bazrgar
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  5. Ardeshir Torab-Jahromi
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  6. Abbas Ghaderi
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Correspondence to Sara Senemar.

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Senemar, S., Doroudchi, M., Pezeshki, A.M. et al. Frequency of cystathionine β-synthase 844INS68 polymorphism in Southern Iran. Mol Biol Rep 36, 353–356 (2009). https://doi.org/10.1007/s11033-007-9186-z

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  • DOI: https://doi.org/10.1007/s11033-007-9186-z

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