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Interactome of signaling networks in wheat: the protein–protein interaction between TaRAR1 and TaSGT1

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Abstract

RAR1 and SGT1 are required for development and disease resistance in plants. In many cases, RAR1 and SGT1 regulate the resistance (R)-gene-mediated defense signaling pathways. Lr21 is the first identified NBS-LRR-type R protein in wheat and is required for resistance to the leaf rust pathogen. The Lr21-mediated signaling pathways require the wheat homologs of RAR1, SGT1, and HSP90. However, the molecular mechanisms of the Lr21-mediated signaling networks remain unknown. Here I present the DNA and protein sequences of TaRAR1 and TaSGT1, and demonstrate for the first time a direct protein-protein interaction between them.

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Abbreviations

HSP90:

Heat shock protein 90

RAR1:

Required for Mla12 resistance

SGT1:

Suppressor of the G2 allele of skp1

Y2H:

Yeast two-hybrid analysis

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Acknowledgements

The author would like to thank J. Hauff for technical support and Dr. L. Huang and Dr. B.S. Gill of Kansas State University for the wheat seeds. I feel a deep sense of gratitude to Dr. Brady Vick, Dr. Lynn Dahleen, and Dr. Michael Edwards of USDA-ARS and the three anonymous reviewers for critical and helpful comments on this manuscript. This work was funded by USDA-ARS CRIS project no. 5442-21000-030-00D.

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  1. Cereal Crops Research Unit, USDA-ARS, Fargo, ND, 58105-5677, USA

    Yin-Shan Tai

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Correspondence to Yin-Shan Tai.

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Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture.

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Tai, YS. Interactome of signaling networks in wheat: the protein–protein interaction between TaRAR1 and TaSGT1. Mol Biol Rep 35, 337–343 (2008). https://doi.org/10.1007/s11033-007-9091-5

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  • DOI: https://doi.org/10.1007/s11033-007-9091-5

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