Skip to main content
SpringerLink
Log in

Probing RNA–antibiotic interactions: a FTIR study

  • Original Paper
  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

The crystal structure determination of antibiotic binding sites on the 30S ribosomal subunit and the increasing demand for developing RNA-based drugs has prompted us to study the direct binding of spectinomycin, vancomycin and bleomycin with yeast total RNA using Fourier transform infrared (FTIR) spectroscopy. We report that the OH of spectinomycin and the peptide group of vancomycin can bind to the bases of RNA, which might depend on Mg2+ concentration. Bleomycin on the other hand does not show such a drastic effect on yeast total RNA. This study might help in developing innovative strategies utilizing RNA molecules to perform a variety of essential biological functions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  • Carter B, de Vroom E, Long EC, Van der Marel GA, Van Boom JH, Hecht SM (1990) Site-specific cleavage of RNA by Fe (II) Bleomycin. Proc Natl Acad Sci USA 87:9373–9377

    Article  PubMed  CAS  Google Scholar 

  • Claussen CA, Long EC (1999) Nucleic acid recognition by metal complexes of bleomycin. Chem Rev 99(9):2797–2816

    Article  PubMed  CAS  Google Scholar 

  • Djoman MC, Neault JF, Hashemi-Fesharaky S, Tajimir-Riahi HA (1998) RNA-ascorbate interaction. J Biomol Str Dyn 15:1115–1119

    CAS  Google Scholar 

  • Goodisman J, Kirk C, Dabrowiak JC (1997) Kinetic analysis of drug cleavage of closed-circular DNA. Biophys Chem 69(2–3):249–268

    Article  PubMed  CAS  Google Scholar 

  • Holmes CE, Carter BJ, Hecht SM (1993) Characterization of Fe (II)-bleomycin RNA strand scission. Biochemistry 32:4293–4307

    Article  PubMed  CAS  Google Scholar 

  • Johnson IM, Kumar SGB, Malathi R (2003a) RNA binding efficacy of theophylline, theobromine and caffeine. J Biomol Str Dyn 20:687–692

    CAS  Google Scholar 

  • Park IK, Kim JY, Lim EH, Shin S (2000) Spectinomycin inhibits the self-splicing of the Group I intron RNA. Biochem Biophysic Res Com 269:574–579

    Article  CAS  Google Scholar 

  • Sammes PG (1977) Topics in antibiotic chemistry, vol 1. Sussex, England, pp 24

  • Stubbe J, Kozarich JW (1987) Mechanisms of Bleomycin-induced DNA-degradation. Chem Rev 87(5):1107–1136

    Article  CAS  Google Scholar 

  • Zimmerman JM, Maher LJ III (2002) In vivo selection of spectinomycin-binding RNAs. Nucleic Acids Res 30(24):5425–5435

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgement

We thank Prof. R. Raghunathan, Head, Department of Organic Chemistry for his help in the FTIR studies.

Author information

Authors and Affiliations

  1. Department of Genetics, Dr ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai, 600 113, India

    J. Prathiba & R. Malathi

Authors
  1. J. Prathiba
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Malathi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to R. Malathi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Prathiba, J., Malathi, R. Probing RNA–antibiotic interactions: a FTIR study. Mol Biol Rep 35, 51–57 (2008). https://doi.org/10.1007/s11033-006-9051-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-006-9051-5

Keywords

Search

Navigation