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A novel gene STYK1/NOK is upregulated in estrogen receptor-alpha negative estrogen receptor-beta positive breast cancer cells following estrogen treatment

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Abstract

The human STYK1/NOK protein is approximately 30–35% similar to mouse fibroblast growth factor receptor 3 and a kinase homologue in D. melanogaster in the tyrosine protein kinase region. STYK1/NOK was identified as being up regulated in MDA-MB-231, an estrogen receptor-alpha negative breast cancer cell line, following 12 h of estrogen treatment at 1 × 10−9 M. On further investigation of STYK1/NOK in estrogen treated cell line MDA-MB-231, STYK1/NOK was up regulated at 6 h post treatment when compared to untreated cells. We also investigated the expression levels of STYK1/NOK in other breast cancer cell lines MCF-7, MDA-MB-231, BT-549, and MDA-MB-435S using QRT-PCR. In addition, the analysis of message accumulation was increased with other synthetic estrogen response modifiers. We propose that the regulation of STYK1/NOK is achieved independent of ERα and suggests further investigation to the relevance of this kinase in breast cancer progression.

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Abbreviations

DES:

Diethylstilbestrol

ERα:

Estrogen receptor alpha

ERβ:

Estrogen receptor beta

FGF:

Fibroblast growth factor

PPT:

1,3,5-Tris94-hydroxyphenyl)-4-propyl-1H-pyrazole

RAL:

Raloxifene hydrochloride

STYK1/NOK:

Serine/Threonine/Tyrosine kinase 1

TAM:

Tamoxifen citrate

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Acknowledgements

The authors would like to thank for support by grants from NCI P20CA091366, NIGMS S06GM008247, NIGMS R25GM060414 and NCRR G12RR003062 and the Avon Foundation. The authors are also grateful to Drs. J. Simons and Tameka Clemons for their editorial assistance.

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Authors and Affiliations

  1. Department of Hematology and Oncology, Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA, 30322, USA

    K. Sean Kimbro, Kaitlin Duschene & Margeret Willard

  2. Department of Biological Sciences, Clark Atlanta University, Atlanta, GA, 30314, USA

    Jodi-Ann Moore & Shalonda Freeman

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  1. K. Sean Kimbro
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  2. Kaitlin Duschene
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  3. Margeret Willard
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  4. Jodi-Ann Moore
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Correspondence to K. Sean Kimbro.

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Kimbro, K.S., Duschene, K., Willard, M. et al. A novel gene STYK1/NOK is upregulated in estrogen receptor-alpha negative estrogen receptor-beta positive breast cancer cells following estrogen treatment. Mol Biol Rep 35, 23–27 (2008). https://doi.org/10.1007/s11033-006-9047-1

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  • DOI: https://doi.org/10.1007/s11033-006-9047-1

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