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Induction of tobacco genes in response to oligochitosan

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Abstract

Oligochitosan has a variety of biological activities. To understand its mechanism, DDRT-PCR, reverse Northern blot and quantitative relative RT-PCR were used to identify and isolate genes whose transcription were altered in cultured Nicotiana tabacum (var. Samsun NN) plants that were treated with oligochitosan. Three genes whose mRNA levels significantly changed in response to oligochitosan were isolated and identified. One gene is up-regulated, and two genes are down-regulated. These genes encode a DNAJ heat shock N-terminal domain-containing protein, a histone H1 gene and a hypothetical protein, whose function is unknown. The results suggest that the usefulness of mRNA differential display technique for the detection of plant metabolic pathways affected by oligochitosan.

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Authors and Affiliations

  1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China

    Fuyun Zhang, Bin Feng, Wei Li, Xuefang Bai, Yuguang Du & Yukui Zhang

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  1. Fuyun Zhang
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  2. Bin Feng
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  3. Wei Li
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  4. Xuefang Bai
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  5. Yuguang Du
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  6. Yukui Zhang
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Correspondence to Yuguang Du.

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Zhang, F., Feng, B., Li, W. et al. Induction of tobacco genes in response to oligochitosan. Mol Biol Rep 34, 35–40 (2007). https://doi.org/10.1007/s11033-006-9008-8

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  • DOI: https://doi.org/10.1007/s11033-006-9008-8

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