Skip to main content

Advertisement

SpringerLink
Log in

Molecular Cloning of a Novel Gene ZAhi-1 and its Expression Analysis during Zebrafish Gametogenesis

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Proto-oncogen Ahi-1 is closely related to a lot of human and mouse diseases. Ahi-1 mutation will lead to leukemia in mice and Joubert syndrome in human beings. We have cloned the full cDNA sequence of Ahi-1 homologous in zebrafish, and RT-PCR results of ZAhi-1 in different tissues reveal that ZAhi-1 expressed highest in the mature gonad. In situ hybridization results of zebrafish gonad show that ZAhi-1 only expressed in the early stages’ gamete cells. RT-PCR analyses of mouse Ahi-1 in different stages of spermatogenesis have been done according to the published Ahi-1 sequence, and the findings reveal that Ahi-1 is expressed in gamete of pachytene stage. It can then be safely concluded that Ahi-1 might take place in the spermatocyte from the early pachytene stage to the late pachytene stage.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Y Poirier C Kozak P Jolioeur (1988) J. Virol. 62 3985–3992 Occurrence Handle2845118 Occurrence Handle1:CAS:528:DyaL1MXhvFKn

    PubMed  CAS  Google Scholar 

  2. J Xiaoyan Z Hanna M Kaouass L Girard P Jolicoeur (2002) J. Virol. 76 IssueID18 9046–9059 Occurrence Handle10.1128/JVI.76.18.9046-9059.2002

    Article  Google Scholar 

  3. Russel J. Ferland & Christopher A. Walsh etc. (2004) Nat. Genet. 36(9): 1008–1013

  4. X Jiang Y Zhao WY Chan S Vercauteren E Pang S Kennedy F Nicolini A Eaves CT Eaves (2004) Blood 103 IssueID10 3897–3904 Occurrence Handle14751929 Occurrence Handle1:CAS:528:DC%2BD2cXjvF2hsr8%3D Occurrence Handle10.1182/blood-2003-11-4026

    Article  PubMed  CAS  Google Scholar 

  5. P Song P Malhotra N Tuteja VS Chauhan (1999) Biochem. Bioph. Res. Co. 255 312–316 Occurrence Handle1:CAS:528:DyaK1MXhsVShtrs%3D Occurrence Handle10.1006/bbrc.1999.0204

    Article  CAS  Google Scholar 

  6. Lv Daoyuan P Song Y Chen W Gong S Mo (2005) Biochem. Bioph. Res. Co. 329 632–637 Occurrence Handle10.1016/j.bbrc.2005.02.023

    Article  Google Scholar 

  7. EJ Neer CJ Schmidt R Nambudripad TF Smith (1994) Nature 371 297–300 Occurrence Handle8090199 Occurrence Handle1:CAS:528:DyaK2cXmvFGgtb0%3D Occurrence Handle10.1038/371297a0

    Article  PubMed  CAS  Google Scholar 

  8. L Voorn Particlevan der HL Ploegh (1992) FEBS Lett. 307 131–134 Occurrence Handle1644165 Occurrence Handle10.1016/0014-5793(92)80751-2

    Article  PubMed  Google Scholar 

  9. BJ Mayer (2001) J. Cell Sci. 114 1253–1263 Occurrence Handle11256992 Occurrence Handle1:CAS:528:DC%2BD3MXjt1Omt7k%3D

    PubMed  CAS  Google Scholar 

  10. Bellvi AR, Cavicchia JC, Milletfe CF, O’brien DA, Bhatnagar YM & Dym M (1977) J. Cell Biol. 74(1): 68–85

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Molecular Genetics and Developmental Biology, College of Life Sciences, Wuhan University, Wuhan, 430072, China

    Wei Zhou & Ping Song

Authors
  1. Wei Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ping Song
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ping Song.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhou, W., Song, P. Molecular Cloning of a Novel Gene ZAhi-1 and its Expression Analysis during Zebrafish Gametogenesis. Mol Biol Rep 33, 111–116 (2006). https://doi.org/10.1007/s11033-006-0005-8

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-006-0005-8

Keywords

Search

Navigation