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Cloning and Characterization of a Novel KRAB-domain-containing Zinc Finger Gene (ZNF284L)†

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Abstract

The zinc finger gene (ZNF) family plays an important role in the regulation of transcription. This study reports the cloning and characterization of a novel human zinc finger protein cDNA (ZNF284L) from fetal brain cDNA library. The ZNF284L cDNA is 2223 bp in length encoding a 593-aa polypeptide. The protein contains a KRAB A+b box and eleven C2H2 type zinc finger motifs. ZNF284L gene is mapped to 19q13.2–19q13.3 with 5 exons, and the expression pattern of ZNF284L gene was also examined by reverse transcription polymerase chain reaction (RT-PCR). The transcripts were detected in the human lung, liver, pancreas, thymus, heart, placenta, spleen, prostate, ovary, small intestine and colon, but in human brain, skeletal muscle, kidney, testis and peripheral blood leukocyte, no expression was detected.

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References

  1. Y Tian GJ Breedveld S Huang BA Oostra P Heutink WH Lo (2002) Biochim. Biophys. Acta 1577 121–125 Occurrence Handle12151103 Occurrence Handle1:CAS:528:DC%2BD38XlvVeqsrY%3D

    PubMed  CAS  Google Scholar 

  2. PJ Mitchell R Tjian (1989) Science. 245 371–378 Occurrence Handle2667136 Occurrence Handle1:CAS:528:DyaL1MXltFWjtb4%3D

    PubMed  CAS  Google Scholar 

  3. VW Yang (1998) J. Nutr. 128 2045–2051 Occurrence Handle9808664 Occurrence Handle1:CAS:528:DyaK1cXnt1Sgsrg%3D

    PubMed  CAS  Google Scholar 

  4. DM Gou Y Sun L Gao LM Chow J Huang YD Feng DH Jiang WX Li (2001) Biochim. Biophys. Acta 1518 306–310 Occurrence Handle11311945 Occurrence Handle1:CAS:528:DC%2BD3MXivVehtbk%3D

    PubMed  CAS  Google Scholar 

  5. J Dai Y Li C Ji F Jin Z Zheng X Wang X Sun X Xu S Gu Y Xie Y Mao (2003) Cytogenet. Genome. Res. 103 74–78 Occurrence Handle15004467 Occurrence Handle1:STN:280:DC%2BD2c7htlKksQ%3D%3D Occurrence Handle10.1159/000076292

    Article  PubMed  CAS  Google Scholar 

  6. H Wu S Zhang W Qiu G Zhang Q Xia C Xiao X Huang M Huang P Agen T Fan J Yang A Milunsky (2001) Biochim. Biophys. Acta 1518 190–193 Occurrence Handle11267678 Occurrence Handle1:CAS:528:DC%2BD3MXitVOks7Y%3D

    PubMed  CAS  Google Scholar 

  7. K Nakayama KW Kim A Miyajima (2002) EMBO J. 21 6174–6184 Occurrence Handle12426389 Occurrence Handle1:CAS:528:DC%2BD38XptFOrsbg%3D Occurrence Handle10.1093/emboj/cdf596

    Article  PubMed  CAS  Google Scholar 

  8. T Evans G Felsenfeld (1989) Cell 58 877–885 Occurrence Handle2776214 Occurrence Handle1:CAS:528:DyaK3cXitlSmt7g%3D Occurrence Handle10.1016/0092-8674(89)90940-9

    Article  PubMed  CAS  Google Scholar 

  9. BC Varnum QF Ma TH Chi B Fletcher HR Herschman (1991) Mol. Cell. Biol. 11 1754–1758 Occurrence Handle1996120 Occurrence Handle1:CAS:528:DyaK3MXlslKhu7g%3D

    PubMed  CAS  Google Scholar 

  10. XA Li K Kokame K Okubo K Shimokado Y Tsukamoto T Miyata H Kato C Yutani (1999) Biochim. Biophys. Acta 1489 405–412 Occurrence Handle10673043 Occurrence Handle1:CAS:528:DC%2BD3cXnt1ajsw%3D%3D

    PubMed  CAS  Google Scholar 

  11. R Schuh W Aicher U Gaul S Cote A Preiss D Maier E Seifert U Nauber C Schroder R Kemler et al. (1986) Cell 47 1025–1032 Occurrence Handle3096579 Occurrence Handle1:CAS:528:DyaL2sXnvFGlug%3D%3D Occurrence Handle10.1016/0092-8674(86)90817-2

    Article  PubMed  CAS  Google Scholar 

  12. KG Becker JW Nagle RD Canning AM Dehejia MH Polymeropoulos AM Gado WE Biddison PD Drew (1997) Genomics 41 502–504 Occurrence Handle9169157 Occurrence Handle1:CAS:528:DyaK2sXjtVShu7o%3D Occurrence Handle10.1006/geno.1997.4678

    Article  PubMed  CAS  Google Scholar 

  13. C Mark M Abrink L Hellman (1999) DNA Cell Biol. 18 381–396 Occurrence Handle10360839 Occurrence Handle1:CAS:528:DyaK1MXjsFyqtLw%3D Occurrence Handle10.1089/104454999315277

    Article  PubMed  CAS  Google Scholar 

  14. B Grondin F Cote M Bazinet M Vincent M Aubry (1997) J. Biol. Chem. 272 27877–27885 Occurrence Handle9346935 Occurrence Handle1:CAS:528:DyaK2sXnt12lsbY%3D Occurrence Handle10.1074/jbc.272.44.27877

    Article  PubMed  CAS  Google Scholar 

  15. NP Pavletich CO Pabo (1991) Science 252 809–817 Occurrence Handle2028256 Occurrence Handle1:CAS:528:DyaK3MXisVeisbY%3D

    PubMed  CAS  Google Scholar 

  16. O Theunissen F Rudt U Guddat H Mentzel T Pieler (1992) Cell 71 679–690 Occurrence Handle1423623 Occurrence Handle1:CAS:528:DyaK3sXhvVahtQ%3D%3D Occurrence Handle10.1016/0092-8674(92)90601-8

    Article  PubMed  CAS  Google Scholar 

  17. E Bellefroid C Bourguignon T Bouwmeester O Rausch B Blumberg T Pieler (1997) Mech. Dev. 63 99–108 Occurrence Handle9178260 Occurrence Handle1:CAS:528:DyaK2sXjvVKitbc%3D Occurrence Handle10.1016/S0925-4773(97)00034-8

    Article  PubMed  CAS  Google Scholar 

  18. EJ Bellefroid DA Poncelet PJ Lecocq O Revelant JA Martial (1991) Proc. Natl. Acad. Sci. USA 88 3608–3612 Occurrence Handle2023909 Occurrence Handle1:CAS:528:DyaK38Xhslegtw%3D%3D Occurrence Handle10.1073/pnas.88.9.3608

    Article  PubMed  CAS  Google Scholar 

  19. H Peng GE Begg SL Harper JR Friedman DW Speicher FJ Rauscher Suffix3rd (2000) J. Biol. Chem. 275 18000–18010 Occurrence Handle10748030 Occurrence Handle1:CAS:528:DC%2BD3cXktlymtro%3D Occurrence Handle10.1074/jbc.M001499200

    Article  PubMed  CAS  Google Scholar 

  20. M Yokoyama M Nakamura K Okubo K Matsubara Y Nishi T Matsumoto A. Fukushima (1997) Biochim. Biophys. Acta 1353 13–17 Occurrence Handle9256059 Occurrence Handle1:CAS:528:DyaK2sXkt1Sjt74%3D

    PubMed  CAS  Google Scholar 

  21. M Rosati M Marino A Franze A Tramontano G Grimaldi (1991) Nucleic Acids Res. 19 5661–5667 Occurrence Handle1945843 Occurrence Handle1:CAS:528:DyaK3sXhtF2isbo%3D

    PubMed  CAS  Google Scholar 

  22. EJ Bellefroid JC Marine T Ried PJ Lecocq M Riviere C Amemiya DA Poncelet PG Coulie P Jong Particlede C Szpirer et al. (1993) EMBO J. 12 1363–1374 Occurrence Handle8467795 Occurrence Handle1:CAS:528:DyaK3sXisV2jtr0%3D

    PubMed  CAS  Google Scholar 

  23. HJ Thiesen E Bellefroid O Revelant JA Martial (1991) Nucleic Acids Res. 19 3996 Occurrence Handle1861988 Occurrence Handle1:CAS:528:DyaK38Xhslensg%3D%3D

    PubMed  CAS  Google Scholar 

  24. B Le Douarin AL Nielsen JM Garnier H Ichinose F Jeanmougin R Losson P Chambon (1996) EMBO J. 15 6701–6715 Occurrence Handle8978696 Occurrence Handle1:CAS:528:DyaK2sXht1yntbY%3D

    PubMed  CAS  Google Scholar 

  25. R Lange A Christoph HJ Thiesen G Vopper KR Johnson L Lemaire M Plomann H Cremer D Barthels UA. Heinlein (1995) DNA Cell Biol. 14 971–981 Occurrence Handle7576184 Occurrence Handle1:CAS:528:DyaK2MXpsFCisro%3D Occurrence Handle10.1089/dna.1995.14.971

    Article  PubMed  CAS  Google Scholar 

  26. JF Margolin JR Friedman WK Meyer H Vissing HJ Thiesen FJ Rauscher Suffix3rd (1994) Proc. Natl. Acad. Sci. USA 91 4509–4513 Occurrence Handle8183939 Occurrence Handle1:CAS:528:DyaK2cXltVWjt7s%3D Occurrence Handle10.1073/pnas.91.10.4509

    Article  PubMed  CAS  Google Scholar 

  27. M Kozak (1987) Nucleic Acids Res. 15 8125–8148 Occurrence Handle3313277 Occurrence Handle1:CAS:528:DyaL2sXmtlSiurg%3D

    PubMed  CAS  Google Scholar 

  28. SD Dreyer Q Zheng B Zabel A Winterpacht B Lee (1999) Genomics 62 119–122 Occurrence Handle10585779 Occurrence Handle1:CAS:528:DyaK1MXns1Klurw%3D Occurrence Handle10.1006/geno.1999.5981

    Article  PubMed  CAS  Google Scholar 

  29. A Preiss UB Rosenberg A Kienlin E Seifert H Jackle (1985) Nature 313 27–32 Occurrence Handle3917552 Occurrence Handle1:CAS:528:DyaL2MXlslWmsw%3D%3D Occurrence Handle10.1038/313027a0

    Article  PubMed  CAS  Google Scholar 

  30. J Miller AD McLachlan A Klug (1985) EMBO J. 4 1609–1614 Occurrence Handle4040853 Occurrence Handle1:CAS:528:DyaL2MXltlKgs78%3D

    PubMed  CAS  Google Scholar 

  31. T el-Baradi T Pieler (1991) Mech. Dev. 35 155–169 Occurrence Handle1768617 Occurrence Handle1:CAS:528:DyaK38Xlt1CitA%3D%3D Occurrence Handle10.1016/0925-4773(91)90015-X

    Article  PubMed  CAS  Google Scholar 

  32. JT Kadonaga KR Carner FR Masiarz R Tjian (1987) Cell 51 1079–1090 Occurrence Handle3319186 Occurrence Handle1:CAS:528:DyaL1cXntVSiug%3D%3D Occurrence Handle10.1016/0092-8674(87)90594-0

    Article  PubMed  CAS  Google Scholar 

  33. UB Rosenberg A Preiss E Seifert H Jackle DC Knipple (1985) Nature 313 703–706 Occurrence Handle2579337 Occurrence Handle1:CAS:528:DyaL2MXhsVCjtL4%3D Occurrence Handle10.1038/313703a0

    Article  PubMed  CAS  Google Scholar 

  34. VJ Bardwell R Treisman (1994) Genes Dev. 8 1664–1677 Occurrence Handle7958847 Occurrence Handle1:CAS:528:DyaK2cXmslegurc%3D

    PubMed  CAS  Google Scholar 

  35. AJ Williams LM Khachigian T Shows T Collins (1995) J. Biol. Chem. 270 22143–22152 Occurrence Handle7673192 Occurrence Handle1:CAS:528:DyaK2MXot1Ghs78%3D Occurrence Handle10.1074/jbc.270.38.22143

    Article  PubMed  CAS  Google Scholar 

  36. T Collins JR Stone AJ Williams (2001) Mol. Cell. Biol. 21 3609–3615 Occurrence Handle11340155 Occurrence Handle1:CAS:528:DC%2BD3MXjs1Oquro%3D Occurrence Handle10.1128/MCB.21.11.3609-3615.2001

    Article  PubMed  CAS  Google Scholar 

  37. R Witzgall E O’Leary A Leaf D Onaldi JV Bonventre (1994) Proc. Natl. Acad. Sci. USA 91 4514–4518 Occurrence Handle8183940 Occurrence Handle1:CAS:528:DyaK2cXkt1ynu74%3D Occurrence Handle10.1073/pnas.91.10.4514

    Article  PubMed  CAS  Google Scholar 

  38. JC Venter MD Adams EW Myers PW Li RJ Mural GG Sutton HO Smith M Yandell CA Evans RA Holt et al. (2001) Science 291 1304–1351 Occurrence Handle11181995 Occurrence Handle1:CAS:528:DC%2BD3MXhtlSgsbo%3D Occurrence Handle10.1126/science.1058040

    Article  PubMed  CAS  Google Scholar 

  39. H Vissing WK Meyer L Aagaard N Tommerup HJ Thiesen (1995) FEBS Lett. 369 153–157 Occurrence Handle7649249 Occurrence Handle1:CAS:528:DyaK2MXnslyiu7s%3D Occurrence Handle10.1016/0014-5793(95)00728-R

    Article  PubMed  CAS  Google Scholar 

  40. G Pengue L Lania (1996) Proc. Natl. Acad. Sci. USA 93 1015–1020 Occurrence Handle8577706 Occurrence Handle1:CAS:528:DyaK28Xptl2nsQ%3D%3D Occurrence Handle10.1073/pnas.93.3.1015

    Article  PubMed  CAS  Google Scholar 

  41. ZG Han QH Zhang M Ye LX Kan BW Gu KL He SL Shi J Zhou G Fu M Mao SJ Chen L Yu Z Chen (1999) J. Biol. Chem. 274 35741–35748 Occurrence Handle10585455 Occurrence Handle1:CAS:528:DyaK1MXotVGntbs%3D Occurrence Handle10.1074/jbc.274.50.35741

    Article  PubMed  CAS  Google Scholar 

  42. SS Kim YM Chen E O’Leary R Witzgall M Vidal JV Bonventre (1996) Proc. Natl. Acad. Sci. USA 93 15299–152304 Occurrence Handle8986806 Occurrence Handle1:CAS:528:DyaK2sXnt1eg Occurrence Handle10.1073/pnas.93.26.15299

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, 200433, People’s Republic of China

    Gang Yin, Chaoneng Ji, Li Zeng, Zebin Wang, Jiayan Wang, Zhouliang Shen, Tong Wu, Shaohua Gu, Yi Xie & Yumin Mao

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  1. Gang Yin
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  2. Chaoneng Ji
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  3. Li Zeng
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Correspondence to Yumin Mao.

Additional information

The nucleotide sequences reported in this paper have been submitted to GenBank under accession number: AY458845

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Yin, G., Ji, C., Zeng, L. et al. Cloning and Characterization of a Novel KRAB-domain-containing Zinc Finger Gene (ZNF284L)† . Mol Biol Rep 33, 137–144 (2006). https://doi.org/10.1007/s11033-005-6405-3

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