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The interaction of PTP-BL PDZ domains with RIL: An enigmatic role for the RIL LIM domain

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Abstract

PDZ domains are protein-protein interaction modules that are crucial for the assembly of structural and signaling complexes. PDZ domains specifically bind short carboxyl-terminal peptides and occasionally internal sequences that structurally resemble peptide termini. Previously, using yeast two-hybrid methodology, we studied the interaction of two PDZ domains present in the large submembranous protein tyrosine phosphatase PTP-BL with the C-terminal half of the LIM domain-containing protein RIL. Deletion of the extreme RIL C-terminus did not eliminate binding, suggesting the presence of a PDZ binding site within the RIL LIM moiety. We have now performed experiments in mammalian cell lysates and found that the RIL C-terminus proper, but not the RIL LIM domain, can interact with PTP-BL, albeit very weakly. However, this interaction with PTP-BL PDZ domains is greatly enhanced when the combined RIL LIM domain and C-terminus is used, pointing to synergistic effects. NMR titration experiments and site-directed mutagenesis indicate that this result is not dependent on specific interactions that require surface exposed residues on the RIL LIM domain, suggesting a stabilizing role in the association with PTP-BL.

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Abbreviations

ALP:

alpha-actinin 2-associated LIM protein

C-terminal:

carboxyl terminal

GFP:

green fluorescent protein

LIM:

acronym of Lin-11 Lsl-1 Mec-3

NMR:

nuclear magnetic resonance

PDZ:

acronym of PSD95/SAP90 DlgA ZO-1

PTP-BL:

Protein tyrosine phosphatase BAS-like

RIL:

reversion-induced LIM gene.

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Authors and Affiliations

  1. Department of Cell Biology, Nijmegen Center for Molecular Life Sciences, University of Nijmegen, Geert Grooteplein 28, 6525 GA, Nijmegen, The Netherlands

    Lieke C. J. van den Berk, Marco A. van Ham, Mariska M. te Lindert & Wiljan J. A. J. Hendriks

  2. Department of Biophysical Chemistry, NSRIM Center, University of Nijmegen, Toernooiveld 1, 6525 ED, Nijmegen, The Netherlands

    Tine Walma, Jan Aelen & Geerten W. Vuister

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  1. Lieke C. J. van den Berk
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  2. Marco A. van Ham
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  3. Mariska M. te Lindert
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  4. Tine Walma
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  7. Wiljan J. A. J. Hendriks
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Correspondence to Wiljan J. A. J. Hendriks.

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van den Berk, L.C.J., van Ham, M.A., te Lindert, M.M. et al. The interaction of PTP-BL PDZ domains with RIL: An enigmatic role for the RIL LIM domain. Mol Biol Rep 31, 203–215 (2005). https://doi.org/10.1007/s11033-005-1407-8

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  • DOI: https://doi.org/10.1007/s11033-005-1407-8

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