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Identification and expression of a conserved ubiquitin gene homologue of Spodoptera litura nucleopolyhedrovirus (SpltNPV-I)

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Abstract

An ORF having a potential to code for a polypeptide of 79 amino acids has been identified within 993 nt sequence of 2 kb EcoRI-W fragment of Spodoptera litura nucleopolyhedrovirus (SpltNPV-I). Nucleotide and deduced amino acid sequence analyses showed its identity with the ubiquitin homologue of eukaryotes (79–80%), Melanoplus sanguinipes entomopoxvirus (76%) and other baculoviruses (72–89%). The ORF is under baculovirus late promoter motif RTAAG but unlike other baculoviruses, three such motifs at −6, −10 and −27 position are present in SpltNPV. The ORF expresses as a 10 kDa protein in E. coli and the purified recombinant protein showed crossreactivity with the rabbit anti-ubiquitin antibodies.

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  1. Institute of Genomics and Integrative Biology (CSIR), Delhi University Campus, Mall Road, 110 007, Delhi, India

    Monika Jain & Rakha Hari Das

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  1. Monika Jain
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  2. Rakha Hari Das
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Correspondence to Rakha Hari Das.

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Jain, M., Das, R.H. Identification and expression of a conserved ubiquitin gene homologue of Spodoptera litura nucleopolyhedrovirus (SpltNPV-I). Mol Biol Rep 31, 261–266 (2005). https://doi.org/10.1007/s11033-005-0887-x

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  • DOI: https://doi.org/10.1007/s11033-005-0887-x

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