Skip to main content
SpringerLink
Log in

Cloning, expression and chromosomal assignment of human pleckstrin 2

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

We report the isolation of human pleckstrin 2 cDNA. The cDNA contains a 1059 bp open reading frame encoding a polypeptide of 353 amino acid residues. The deduced amino acid sequence indicates that pleckstrin 2contains two pleckstrin homology domains and a DEP (dishvelled, egl-10, and pleckstrin) domain and had a 95% identity with the sequence of mouse pleckstrin 2. Northern blot and a reverse transcription-coupled polymerase chain reaction analysis revealed that pleckstrin 2 mRNA is widely expressed in a variety of cell lines. The chromosomal location of the mouse pleckstrin 2 gene was on the D3 band of chromosome 12, as determined by fluorescence in situhybridization and the human pleckstrin 2 gene was mapped to chromosome 14q24.1 by a bioinformatics analysis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

DEP domain:

dishvelled, egl-10, and pleckstrin domain

FISH:

fluorescence in situ hybridization

G3PDH:

glyceraldehyde 3-phosphate dehydrogenase

PCR:

polymerase chain reaction

PH:

pleckstrin homology

PKC:

protein kinase C

RACE:

rapid amplification of cDNA ends

References

  • RM Lyons NL Stanford PW Majerus (1975) J Clin. Invest. 56 924–936 Occurrence Handle1:CAS:528:DyaE2MXls1Wgtbo%3D Occurrence Handle169298

    CAS  PubMed  Google Scholar 

  • M Tyers RA Rachubinski MI Stewart AM Varrichio RGL Shorr RJ Haslam CB Harley (1988) Nature 333 470–473 Occurrence Handle1:CAS:528:DyaL1cXltVGksrY%3D Occurrence Handle2897630

    CAS  PubMed  Google Scholar 

  • RJ Haslam HB Koide BA Hemmings (1993) Nature 363 309–310 Occurrence Handle1:STN:280:ByyB28fjvVQ%3D

    CAS  Google Scholar 

  • BJ Mayer R Ren KL Clark D Baltimore (1993) Cell 73 629–630 Occurrence Handle1:CAS:528:DyaK3sXksVeltb4%3D Occurrence Handle8500161

    CAS  PubMed  Google Scholar 

  • MA Lemmon KM Ferguson (2000) Biochem. J. 350 1–18 Occurrence Handle1:CAS:528:DC%2BD3cXmsVaqtr0%3D Occurrence Handle10926821

    CAS  PubMed  Google Scholar 

  • D Gailani TC Fisher DC Mills DE Macfarlane (1990) Br. J. Haematol. 74 192–202 Occurrence Handle1:CAS:528:DyaK3cXhtlKlsbk%3D Occurrence Handle2317454

    CAS  PubMed  Google Scholar 

  • T Inazu H Yamamura (1999a) Res. Commun. Biochem. Cell Mol. Biol. 3 21–28 Occurrence Handle1:CAS:528:DyaK1MXmvFWqu74%3D

    CAS  Google Scholar 

  • T Inazu K Yamada K Miyamoto (1999b) Biochem. Biophys. Res. Commun. 265 87–93 Occurrence Handle1:CAS:528:DyaK1MXntVCnu78%3D

    CAS  Google Scholar 

  • MH Hu EM Bauman RL Roll N Yielding CS Abrams (1999) J. Biol. Chem. 274 21515–21518 Occurrence Handle1:CAS:528:DyaK1MXltVymsr8%3D Occurrence Handle10419454

    CAS  PubMed  Google Scholar 

  • Y Matsuda VM Chapman (1995) Electrophoresis 16 261–272 Occurrence Handle1:CAS:528:DyaK2MXktVGkurY%3D Occurrence Handle7774567

    CAS  PubMed  Google Scholar 

  • CP Ponting P Bork (1996) Trends Biochem. Sci. 21 245–246 Occurrence Handle1:CAS:528:DyaK28XksVymsbg%3D Occurrence Handle8755244

    CAS  PubMed  Google Scholar 

  • CS Abrams H Wu W Zhao E Belmonte D White LF Brass (1995) J. Biol. Chem. 270 14485–14492 Occurrence Handle1:CAS:528:DyaK2MXmsVSit7k%3D Occurrence Handle7782310

    CAS  PubMed  Google Scholar 

  • AD Ma LF Brass CS Abrams (1997) J. Cell Biol. 136 1071–1079 Occurrence Handle1:CAS:528:DyaK2sXhvVaktL4%3D Occurrence Handle9060471

    CAS  PubMed  Google Scholar 

  • AD Ma CS Abrams (1909) J. Biol. Chem. 274 28730–28735

    Google Scholar 

  • JL Cmarik G Hegamyer B Gerrard M Dean NH Colburn (2000) Genomics 66 204–212 Occurrence Handle1:CAS:528:DC%2BD3cXktVKmsrc%3D Occurrence Handle10860665

    CAS  PubMed  Google Scholar 

  • M Ansar MA Din M Arshad M Sohail M Faiyaz-Ul-Haque S Haque W Ahmad SM Leal (2003) Eur. J. Hum. Genet. 11 77–80 Occurrence Handle1:CAS:528:DC%2BD3sXht12hu74%3D Occurrence Handle12529709

    CAS  PubMed  Google Scholar 

  • M Debiec-Rychter R Sciot P Pauwels E Schoenmakers P Dal Cin A Hagemeijer (2001) Genes Chromosomes Cancer 32 26–32 Occurrence Handle1:CAS:528:DC%2BD3MXmsVOgu7o%3D Occurrence Handle11477658

    CAS  PubMed  Google Scholar 

Download references

Author information

Author notes

  1. Tetsuya Inazu

    Present address: Department of Clinical Research, National Saigata Hospital, Nakakubiki, Niigata, 949-3193, Japan

Authors and Affiliations

  1. Department of Biochemistry, Fukui Medical University, Matsuoka, Fukui, Japan

    Tetsuya Inazu & Kaoru Miyamoto

  2. Laboratory of Cytogenetics, Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Kita, Sapporo, Japan

    Asato Kuroiwa & Yoichi Matsuda

  3. Chromosome Research Unit, Faculty of Science, Hokkaido University, Kita, Sapporo, Japan

    Yoichi Matsuda

Authors
  1. Tetsuya Inazu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Asato Kuroiwa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yoichi Matsuda
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kaoru Miyamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tetsuya Inazu.

Additional information

The nucleotide sequence data reported in this paper will appear in the DDBJ, EMBL, and the GenBank nucleotide sequence databases with the following Accession Number AF228603.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Inazu, T., Kuroiwa, A., Matsuda, Y. et al. Cloning, expression and chromosomal assignment of human pleckstrin 2 . Mol Biol Rep 32, 35–40 (2005). https://doi.org/10.1007/s11033-004-4747-x

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-004-4747-x

Keywords

Search

Navigation