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Overexpression of ERp29 in the thyrocytes of FRTL-5 cells

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Abstract

It was previously reported that the up-regulation of ERp29 mRNA depends on the levels of thyroid stimulating hormone (TSH) in the thyrocytes of FRTL-5 cells. In order to investigate the putative new function of ERp29 as an endoplasmic molecular (ER) chaperone, an ERp29-overexpressing FRTL-5 cell line was established. This cell line had approximately three times the levels of ERp29 protein and an enhanced level of thyroglobulin (Tg) secretion. The results showed both enhanced ERp29 expression and an interaction with the other ER chaperones such as GRP94, BiP, ERp72 and calnexin. In addition, ERp29 enhanced the expression of PKR-like ER kinase (PERK), which is a transmembrane protein located in the ER membrane. These findings suggest that ERp29 assists in protein folding as well as in the secretion of the secretory/plasma membrane proteins under close co-operation with other ER chaperones and the ER stress signaler, PERK.

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Authors and Affiliations

  1. Department of Anatomy, Chungnam National University, Taejon, 301-747, Korea

    Soojung Park & O-Yu Kwon

  2. Department of Biology, Chungnam National University, Taejon, 301-747, Korea

    Kwan-Hee You

  3. Departments of Internal Medicine, Chungnam National University, Taejon, 301-747, Korea

    Minho Shong

  4. Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, RDA, Suwon, 441-100, Korea

    Tae Won Goo, Eun Young Yun & Seok Woo Kang

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  1. Soojung Park
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  2. Kwan-Hee You
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  3. Minho Shong
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  4. Tae Won Goo
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  5. Eun Young Yun
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  6. Seok Woo Kang
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  7. O-Yu Kwon
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Correspondence to O-Yu Kwon.

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Park, S., You, KH., Shong, M. et al. Overexpression of ERp29 in the thyrocytes of FRTL-5 cells. Mol Biol Rep 32, 7–13 (2005). https://doi.org/10.1007/s11033-004-3069-3

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  • DOI: https://doi.org/10.1007/s11033-004-3069-3

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