Development of InDel markers of Bph3 and pyramiding of four brown planthopper resistance genes into an elite rice variety

Abstract

Brown planthopper (BPH) is one of the most destructive pests in rice. Breeding resistant cultivars is the most effective strategy to control BPH. Bph3 is a broad-spectrum and durable BPH resistance locus spanning ~ 50 kb on the short arm of rice chromosome 4. Using the genomic variation data of 4726 rice varieties from public database, we developed 8 InDel markers within the Bph3 locus. By analyzing 563 rice varieties, we demonstrated that these markers could effectively identify Bph3-carriers, with an accuracy rate near to 90%. Through marker-assisted backcross breeding (MABC), we introduced four BPH resistance genes (Bph3/14/18/32) into an elite rice variety, Guang 8B. The results showed that Bph3 had the biggest effect, followed by Bph14 and Bph32, and Bph18 had the smallest effect. In addition, the level of BPH resistance increased with the number of genes pyramided. However, in the case of only two genes being pyramided, the resistance increase was not significant in comparison with the larger effect of the two genes. This suggests that the resistance effects are not simply additive, but epistasis may be involved. The InDel markers and pyramiding lines developed in this study will facilitate Bph3-carrier identification and BPH resistance breeding in rice.

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Funding

This work was supported in part by National Key R&D Program of China (2017YFD0100103), the Distinguished Young Scientific Research Talents Program of Fujian Agriculture and Forestry University (xjq201812) and Fujian Provincial Natural Science Foundation (2017J01437), and the International Sci-Tech Cooperation and Exchange Program of FAFU (KXGH17014).

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LH and JH performed experiments and analyzed the data; LZ and QLH collected plant materials and performed experiments; XCS participated in handling figures and tables; JH and WRW, the co-correspondence authors, designed this research and wrote the manuscript. All authors have approved the manuscript.

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Correspondence to Weiren Wu or Jie Hu.

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He, L., Zou, L., Huang, Q. et al. Development of InDel markers of Bph3 and pyramiding of four brown planthopper resistance genes into an elite rice variety. Mol Breeding 40, 95 (2020). https://doi.org/10.1007/s11032-020-01175-z

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Keywords

  • Rice
  • Bph3
  • InDel markers
  • Gene pyramiding