Northern corn leaf blight (NCLB) is one of the main diseases of maize, which greatly reduces production and causes millions of dollars in losses worldwide annually. Identification of NCLB resistance genes in maize plays an important role in the crop disease resistance breeding. In this study, a BC1F1 population with 1290 individuals was constructed using high resistance line CML493 and high susceptible line PH4CV. In total, 50 resistant individuals and 50 susceptible individuals from the BC1F1 population were selected to construct 4 DNA pools (also with the two parents) which were used for high-throughput sequencing. The key candidate genes associated with NCLB resistance genes were identified using BSA-seq and KASP platforms. The results showed that 3 genes including CDPK21, HEX9, and MKKK18 were identified and this prediction was further verified by RT-qPCR. The annotation functions were sugar signaling, calcium signaling, and MAPK signaling pathways. This research provided key NCLB resistance candidate genes and also provided important markers for disease resistance breeding in maize.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
Tax calculation will be finalised during checkout.
Bergquist RR, Masias OR (1974) Physiologic specialization in Trichometasphaeria turcica f. sp. zeae and T. turcica f. sp. sorghi in Hawaii. Phytopathology 64:645–649
Cabrera JC, Wégria G, Onderwater RCA, González G, Nápoles MC, Falcón-Rodríguez AB, Costales D, Rogers HJ, Diosdado E, González S (2013) Practical use of oligosaccharins in agriculture. Acta Hortic 195–212 . https://doi.org/10.17660/ActaHortic.2013.1009.24
Dingerdissen AL, Geiger HH, Lee M, Schechert A, Welz HG (1996) Interval mapping of genes for quantitative resistance of maize to Setosphaeria turcica, cause of northern leaf blight, in a tropical environment. Mol Breed 2:143–156
Freymark PJ, Lee M, Woodman WL, Martinson CA (1993) Quantitative and qualitative trait loci affecting host-plant response to Exserohilum turcicum in maize (Zea mays L.). Theor Appl Genet 87:537–544. https://doi.org/10.1007/BF00221876
Giovannoni JJ, Wing RA, Ganal MW, Tanksley SD (1991) Isolation of molecular markers from specific chromosomal intervals using DNA pools from existing mapping populations. Nucleic Acids Res 19:6553–6568
Granot D, David-Schwartz R, Kelly G (2013) Hexose kinases and their role in sugar-sensing and plant development. Front Plant Sci 4. https://doi.org/10.3389/fpls.2013.00044
Hooker AL (1977) A second major gene locus in corn for chlorotic-lesion resistance to Helminthosporium turicum 1. Crop Sci 17:132–135
Hooker AL, Tsung YK (1980) Relationship of dominant genes in corn for chlorotic lesion resistance to Helminthosporium turcicum. Plant Dis 64:387–388
Hurni S, Scheuermann D, Krattinger SG, Kessel B, Wicker T, Herren G, Fitze MN, Breen J, Presterl T, Ouzunova M, Keller B (2015) The maize disease resistance gene Htn1 against northern corn leaf blight encodes a wall-associated receptor-like kinase. Proc Natl Acad Sci 112:8780–8785. https://doi.org/10.1073/pnas.1502522112
Jagadeesan R, Fotheringham A, Ebert PR, Schlipalius DI (2013) Rapid genome wide mapping of phosphine resistance loci by a simple regional averaging analysis in the red flour beetle, Tribolium castaneum. BMC Genomics 14:650
(Kazuya Ichimura et al.) MG, Ichimura K, Shinozaki K, Tena G, Sheen J, Henry Y, Champion A, Kreis M, Zhang S, Hirt H, Wilson C, Heberle-Bors E, Ellis BE, Morris PC, Innes RW, Ecker JR, Scheel D, Klessig DF, Machida Y, Mundy J, Ohashi Y, Walker JC (2002) Mitogen-activated protein kinase cascades in plants: a new nomenclature. Trends Plant Sci 7:301–308 . https://doi.org/10.1016/S1360-1385(02)02302-6
Kozak M (1987) An analysis of 5′-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res 15:8125–8148. https://doi.org/10.1093/nar/15.20.8125
Leath S, Pedersen WL (1986) Effects of the Ht, Ht2, and/or Ht3 genes in three maize inbreds on quantitative resistance to Exserohilum turcicum race 2. Plant Dis 70:529–531
Lee J-Y, Yoo B-C, Harmon AC (1998) Kinetic and calcium-binding properties of three calcium-dependent protein kinase isoenzymes from soybean. Biochemistry 37:6801–6809. https://doi.org/10.1021/bi980062q
Li J, Lee Y-RJ, Assmann SM (1998) Guard cells possess a calcium-dependent protein kinase that phosphorylates the KAT1 potassium channel. Plant Physiol 116:785–795. https://doi.org/10.1104/pp.116.2.785
Lu H, Lin T, Klein J, Wang S, Qi J, Zhou Q, Sun J, Zhang Z, Weng Y, Huang S (2014) QTL-seq identifies an early flowering QTL located near flowering locus T in cucumber. Theor Appl Genet 127:1491–1499
Pataky JK, Perkins JM, Leath S (1986) Effects of qualitative and quantitative resistance on the development and spread of northern leaf blight of maize caused by Exserohilum turcicum races 1 and 2. Phytopathology 76:1349–1352
Patterson EL, Fleming MB, Kessler KC, Nissen SJ, Gaines TA (2017) A KASP genotyping method to identify northern watermilfoil, Eurasian watermilfoil, and their interspecific hybrids. Front Plant Sci 8:752
Perkins JM, JM P, AL H (1981) Reactions of eighty-four sources of chlorotic lesion resistance in corn to three biotypes of Helminthosporium turcicum
Perkins JM, Pedersen WL (1987) Disease development and yield losses associated with northern leaf blight on corn. Plant Dis 71:940–943
Proels RK, Hückelhoven R (2014) Cell-wall invertases, key enzymes in the modulation of plant metabolism during defence responses. Mol Plant Pathol 15:858–864. https://doi.org/10.1111/mpp.12139
Putnam-Evans CL, Harmon AC, Cormier MJ (1990) Purification and characterization of a novel calcium-dependent protein kinase from soybean. Biochemistry 29:2488–2495. https://doi.org/10.1021/bi00462a008
Qureshi N, Bariana HS, Zhang P, McIntosh R, Bansal UK, Wong D, Hayden MJ, Dubcovsky J, Shankar M (2018) Genetic relationship of stripe rust resistance genes Yr34 and Yr48 in wheat and identification of linked KASP markers. Plant Dis 102:413–420
Raymundo AD, AD R, AL H (1981) Effect of gene HtN on the development of northern corn leaf blight epidemics
Schechert AW, Welz HG, Geiger HH (1999) QTL for resistance to Setosphaeria turcica in tropical African maize. Crop Sci 39:514–523
Semagn K, Babu R, Hearne S, Olsen M (2014) Single nucleotide polymorphism genotyping using Kompetitive Allele Specific PCR (KASP): overview of the technology and its application in crop improvement. Mol Breed 33:1–14
Takagi H, Abe A, Yoshida K, Kosugi S, Natsume S, Mitsuoka C, Uemura A, Utsushi H, Tamiru M, Takuno S, Innan H, Cano LM, Kamoun S, Terauchi R (2013) QTL-seq: rapid mapping of quantitative trait loci in rice by whole genome resequencing of DNA from two bulked populations. Plant J 74:174–183. https://doi.org/10.1111/tpj.12105
Takagi H, Tamiru M, Abe A, Yoshida K, Uemura A, Yaegashi H, Obara T, Oikawa K, Utsushi H, Kanzaki E (2015) MutMap accelerates breeding of a salt-tolerant rice cultivar. Nat Biotechnol 33:445–449
Thakur RP, Leath S, Leonard KJ (1989) Resistance effects of temperature and light on virulence of Exserohilum turcicum on corn. Phytopathology 79:631–635
Trewavas A, Malho R (1997) Signal perception and transduction: the origin of the phenotype. Plant Cell 9:1181–1195
Welz HG, Geiger HH (2000) Genes for resistance to northern corn leaf blight in diverse maize populations. Plant Breed 119:1–14
Welz HG, Schechert AW, Geiger HH (1999a) Dynamic gene action at QTLs for resistance to Setosphaeria turcica in maize. Theor Appl Genet 98:1036–1045
Welz HG, Xia XC, Bassetti P, Melchinger AE, Lübberstedt T (1999b) QTLs for resistance to Setosphaeria turcica in an early maturing dent$\times$ Flint maize population. Theor Appl Genet 99:649–655
Xu S-M, Brill E, Llewellyn DJ, Furbank RT, Ruan Y-L (2012) Overexpression of a potato sucrose synthase gene in cotton accelerates leaf expansion, reduces seed abortion, and enhances fiber production. Mol Plant 5:430–441. https://doi.org/10.1093/mp/ssr090
Xu X, Ji J, Xu Q, Qi X, Weng Y, Chen X (2018) The major-effect QTL CsARN6. 1 encodes an AAA-ATPase domain-containing protein that is associated with waterlogging stress tolerance through promoting adventitious root formation. Plant J 93:
Yang P, Praz C, Li B, Singla J, Robert CAM, Kessel B, Scheuermann D, Lüthi L, Ouzunova M, Erb M, Krattinger SG, Keller B (2019) Fungal resistance mediated by maize wall-associated kinase Zm WAK - RLK 1 correlates with reduced benzoxazinoid content. New Phytol 221:976–987. https://doi.org/10.1111/nph.15419
This research was funded by the Project of Science and Technology Department of Jilin Province (20180201026NY).
Conflict of interest
The authors declare that they have no conflict of interest.
Consent to participate
All listed authors participated meaningfully in the study and approved the final manuscript.
Consent for publication
The authors have read and approved the final submitted manuscript.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Li, C., Ling, F., Su, G. et al. Location and mapping of the NCLB resistance genes in maize by bulked segregant analysis (BSA) using whole genome re-sequencing. Mol Breeding 40, 92 (2020). https://doi.org/10.1007/s11032-020-01171-3
- Disease resistance
- Annotation function