Abstract
Male reproductive development is vital for crop seed production. However, the mechanism underlying remains largely unknown. Here, we isolated a male sterile mutant osgelp34-1 in rice. Cytological analysis indicated that the mutant exhibited delayed degradation of anther wall layers and abnormal pollen development, which resulted in the production of shrunk and non-viable pollen grains. MutMap and CRISPR/Cas9 analysis confirmed that a point mutation in OsGELP34 is responsible for the male sterile phenotype. OsGELP34 is highly expressed in reproductive tissues and encodes a putative GDSL lipase. OsGELP34 protein is located to the endoplasmic reticulum (ER) and conserved in land plants. Collectively, our findings elaborated that OsGELP34 plays a vital role in rice male reproduction and has potential applications in rice hybrid breeding.
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Funding
This work was supported by the National Natural Science Foundation of China (31901482 and 31971863), the Open Research Fund of State Key Laboratory of Hybrid Rice (Hunan Hybrid Rice Research Centre, 2016KF10), the Sichuan Science and Technology Support Project (2016NZ0103 and 2017NZDZX0001), and the National Key Research and Development Program of China (2017YFD0100201).
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S.L. and P. L. designed and directed the experiments. Z. H. and Y. T. performed the expression analysis and tissue localization and subcellular localization. J.Z., Q.D., and S.W. performed the genetic transformations. D.Z., H.Z., S.L., R.L., and M.Z. performed the phenotypic characterization of the mutant and the transgenic plants. Y.L., A. Z., A.W., and L.W. constructed all the vectors. G. Y., T. Z., X. Z., M.L., and T. L. performed the cloning and functional analysis and collected almost all the data. T. Z. and G. Y. analyzed the data and wrote the manuscript.
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Yuan, G., Zou, T., Zhang, X. et al. A rice GDSL esterase/lipase protein (GELP) is required for anther and pollen development. Mol Breeding 40, 90 (2020). https://doi.org/10.1007/s11032-020-01170-4
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DOI: https://doi.org/10.1007/s11032-020-01170-4