Abstract
FAE1, the gene encoding fatty acid elongase, has been implicated to play a decisive role in the synthesis of erucic acid. Allelic variations in this gene, such as single nucleotide polymorphisms (SNPs), insertions/deletions (InDels), and insertions of transposable element (TE), are predominantly responsible for variability in erucic acid content in the seed, among the Brassica genotypes. Brassica juncea being an amphidiploid, contains two paralogs, FAE1.1 and FAE1.2. Although the coding DNA sequences (CDS) of FAE gene is highly conserved (> 95% identical), the promoter region is found to be polymorphic. The sequence variability in the promoter region of FAE1 across different Brassica spp. suggests their likely involvement in the regulation of the gene expression. This study was aimed to evaluate polymorphism in the upstream region of FAE1.1 and FAE1.2 genes, across the low erucic acid (LEA) and high erucic acid (HEA) cultivars, and to develop molecular markers based on this variability. The upstream regions of FAE were sequenced from LEA cultivar “Pusa Mustard 30” and HEA cultivar “Pusa Bold.” A 28-bp deletion in the promoter of FAE1.1 and a 340-bp insertion of a transposon-like element in the FAE1.2 gene promoter were discovered in LEA genotype. Markers based on the sequence variability in the promoter regions of FAE1.1 and FAE1.2 were found to completely co-segregate with the seed erucic acid content. These markers can be effectively used in marker-assisted selection for development of low erucic acid cultivars in B. juncea.
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The authors thank the Indian Council of Agricultural Research, Govt. of India, for the financial assistance to carry out this research.
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SV, NS, and DK conceived the project. All co-authors, NS, YP, NSH, MK, SY, RS, and SS were involved in executing the experiments. SV, NS, and YP were involved in the drafting of the manuscript. NSH, RB, and DK revised the final manuscript.
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Fig. S1
Sequence alignment of the upstream promoter region of FAE1.1 of B. juncea genotypes Pusa Bold (HEA type), and Pusa Mustard 30 (LEA type) with B. rapa sequences (PNG 78 kb)
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(TIF 59 kb)
Fig. S2
Insertion sequence of the Tc1/Mariner transposon in the promoter region of FAE1.2 (DOCX 12 kb)
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Saini, N., Yashpal, Koramutla, M.K. et al. Promoter polymorphism in FAE1.1 and FAE1.2 genes associated with erucic acid content in Brassica juncea. Mol Breeding 39, 75 (2019). https://doi.org/10.1007/s11032-019-0971-x
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DOI: https://doi.org/10.1007/s11032-019-0971-x