QTL mapping of Fusarium head blight resistance and deoxynivalenol accumulation in the Kansas wheat variety ‘Everest’

Lemes da Silva, Cristiano; Fritz, Allan; Clinesmith, Marshall; Poland, Jesse; Dowell, Floyd; Peiris, Kamaranga

doi:10.1007/s11032-019-0937-z

Published: 16 February 2019

QTL mapping of Fusarium head blight resistance and deoxynivalenol accumulation in the Kansas wheat variety ‘Everest’

Cristiano Lemes da Silva ORCID: orcid.org/0000-0001-7291-4141¹,
Allan Fritz¹,
Marshall Clinesmith¹,
Jesse Poland²,
Floyd Dowell³ &
Kamaranga Peiris¹

Molecular Breeding volume 39, Article number: 35 (2019) Cite this article

989 Accesses
10 Citations
5 Altmetric
Metrics details

Abstract

Fusarium head blight (FHB) reduces grain yield and accumulates mycotoxins in wheat kernels and flour and thus compromise the end-use quality. In this study, quantitative trait loci (QTL) associated with components of FHB resistance were mapped in the Kansas wheat variety Everest. A doubled haploid (DH) population with 176 lines was developed from a cross between Everest and WB-Cedar, which are moderately resistant and moderately susceptible to FHB, respectively. Two-year field evaluations were conducted using a randomized complete block design with three replications. Deoxynivalenol (DON) accumulation and Fusarium-damaged kernels (FDK) were estimated using a single-kernel near-infrared (SKNIR) system. Four QTL from Everest were identified on 1BS, 3DS, 4BL, and 5AS, indicating that this variety is a source of native FHB resistance conditioned by multiple loci. A large-effect QTL (Qksu-fhbE-5AS) from Everest explained up to 13% of the DON variation in the 2-year experiment average. A single QTL from WB-Cedar, Qksu.fhbC-1AS, was identified contributing FHB resistance. QTL grouping analysis showed that individuals containing multiple QTL were significantly more resistant, indicating that QTL pyramiding enhances FHB resistance. A 36% reduction in DON content was observed in DH lines carrying all mapped QTL for DON accumulation. Further validation of the QTL mapped here will allow breeding programs to increase FHB resistance in other genetic backgrounds. This is the first study that identified QTL associated with low DON accumulation in multiple years of field experiment using the SKNIR technology, supporting the use of this methodology in future genetics and breeding studies.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

References

Arruda MP, Brown P, Brown-Guedira GB, Krill AM, Thurber C, Merrill KR, Foresman BJ, Kolb FL (2016) Genome-wide association mapping of Fusarium head blight resistance in wheat using genotyping-by-sequencing. Plant Genome 9:1–14. https://doi.org/10.3835/plantgenome2015.04.0028
CAS Article Google Scholar
Bayer MM, Rapazote-Flores P, Ganal M, Hedley PE, Macaulay M, Plieske J, Ramsay L, Russell J, Shaw PD, Thomas W, Waugh R (2017) Development and evaluation of a barley 50k iSelect SNP array. Front Plant Sci 8:1792. https://doi.org/10.3389/fpls.2017.01792
Article PubMed PubMed Central Google Scholar
Bernardo, R (2009) Should maize doubled haploids be induced among F1 or F2 plants? Theor Appl Genet (2009) 119: 255–262. doi: https://doi.org/10.1007/s00122-009-1034-1
Bockus WW, Appel JA, De Wolf ED, Todd TC, Davis MA, Fritz AK (2015) Impact of wheat cultivar Everest on yield loss in Kansas from Fusarium head blight during 2015. Proceedings of the 2015 National Fusarium Head Blight Forum. St Louis MO
Bradbury PJ, Zhang Z, Kroon DE, Casstevens TM, Ramdoss Y, Buckler ES (2007) TASSEL: software for association mapping of complex traits in diverse samples. Bioinformatics 23:2633–2635. https://doi.org/10.1093/bioinformatics/btm308
CAS Article PubMed Google Scholar
Broman K, Sen S (2009) A guide to QTL mapping with R/qtl. Springer, Berlin. https://doi.org/10.1007/978-0-387-92125-9
Book Google Scholar
Broman KW, Wu H, Sen S, Churchill GA (2003) R/qtl: QTL mapping in experimental crosses. Bioinformatics 19:889–890. https://doi.org/10.1093/bioinformatics/btg112
CAS Article PubMed Google Scholar
Buerstmayr H, Ban T, Anderson JA (2009) QTL mapping and marker-assisted selection for Fusarium head blight resistance in wheat: a review. Plant Breed 128:1–26. https://doi.org/10.1111/j.1439-0523.2008.01550.x
CAS Article Google Scholar
Buerstmayr M, Steiner B, Wagner C, Schwarz P, Brugger K, Barabaschi D, Volante A, Valè G, Cattivelli L, Buerstmayr H (2017) High-resolution mapping of the pericentromeric region on wheat chromosome arm 5AS harboring the Fusarium head blight resistance QTLQfhs.ifa-5A. Plant Biotechnol J 16:1046–1056. https://doi.org/10.1111/pbi.12850
CAS Article PubMed PubMed Central Google Scholar
Burt C, Steed A, Gosman N, Lemmens M, Bird N, Ramirez-Gonzalez R, Holdgate S, Nicholson P (2015) Mapping a type 1 FHB resistance on chromosome 4AS of Triticum macha and deployment in combination with two type 2 resistances. Theor Appl Genet 128(9):1725–1738. https://doi.org/10.1007/s00122-015-2542-9
CAS Article PubMed PubMed Central Google Scholar
Cai J (2016) Meta-analysis of QTL for Fusarium head blight resistance in Chinese wheat landraces using genotyping by sequencing. Dissertation, Kansas State University
Cai J, Wang S, Li T, Zhang G, Bai G (2016) Multiple minor QTL are responsible for Fusarium head blight resistance in Chinese wheat landrace Haiyanzhong. PLoS One 11:e0163292. https://doi.org/10.1371/journal.pone.0163292
CAS Article PubMed PubMed Central Google Scholar
Clinesmith M (2016) Genetic mapping of QTL for Fusarium head blight resistance in winter wheat cultivars Art and Everest. Master Thesis, Kansas State University
Costa JM, Bockelman HE, Brown-Guedira G, Cambron SE, Chen X, Cooper A, Cowger C, Dong Y, Grybauskas A, Jin Y, Kolmer J, Murphy JP, Sneller C, Souza E (2010) Registration of the soft red winter wheat germplasm MD01W233–06–1 resistant to Fusarium head blight. J Plant Reg 4:255–260. https://doi.org/10.3198/jpr2010.01.0034crg
Article Google Scholar
Cuthbert PA, Somers DJ, Brulé-Babel A (2007) Mapping of Fhb2 on chromosome 6BS: a gene controlling Fusarium head blight field resistance in bread wheat (Triticum aestivum L.). Theor Appl Genet 114:429–437. https://doi.org/10.1007/s00122-006-0439-3
CAS Article PubMed Google Scholar
De Wolf ED, Bockus WW, Whitworth RJ (2015) Wheat variety disease and insect rating 2015. Kansas State University, Manhattan, KS
Google Scholar
Dhokane D, Karre S, Kushalappa AC, McCartney C (2016) Integrated metabolo-transcriptomics reveals Fusarium head blight candidate resistance genes in wheat QTL-Fhb2. PLoS One 11:e0155851. https://doi.org/10.1371/journal.pone.0155851
CAS Article PubMed PubMed Central Google Scholar
Dowell FE, Ram MS, Seitz LM (1999) Predicting scab, vomitoxin, and ergosterol in single wheat kernels using near-infrared spectroscopy. Cereal Chem 76:573–576. https://doi.org/10.1094/CCHEM.1999.76.4.573
CAS Article Google Scholar
Dowell FE, Maghirang EB, Graybosch RA, Baenziger PS, Baltensperger DD, Hansen LE (2006) An automated near-infrared system for selecting individual kernels based on specific quality characteristics. Cereal Chem 83:537–543. https://doi.org/10.1094/CC-83-0537
CAS Article Google Scholar
Dowell FE, Maghirang EB, Baenziger PS (2009) Automated single-kernel sorting to select for quality traits in wheat breeding lines. Cereal Chem 86:527–533. https://doi.org/10.1094/CCHEM-86-5-0527
CAS Article Google Scholar
Draeger R, Gosman N, Steed A, Chandler E, Thomsett M, Schondelmaier J, Buerstmayr H, Lemmens M, Schmolke M, Mesterhazy A, Nicholson P (2007) Identification of QTL for resistance to Fusarium head blight, DON accumulation and associated traits in the winter wheat variety Arina. Theor Appl Genet 115:617–625. https://doi.org/10.1007/s00122-007-0592-3
CAS Article PubMed Google Scholar
Dweba CC, Figlan S, Shimelis HA, Motaung TE, Sydenham S, Mwadzingeni L, Tsilo TJ (2017) Fusarium head blight of wheat: pathogenesis and control strategies. Crop Prot 91:114–122. https://doi.org/10.1016/j.cropro.2016.10.002
CAS Article Google Scholar
Eckard J, Gonzalez-Hernandez J, Caffe M, Berzonsky W, Bockus W, Marais G, Baenziger P (2015) Native Fusarium head blight resistance from winter wheat cultivars ‘Lyman,’ ‘Overland,’ ‘Ernie,’ and ‘Freedom’ mapped and pyramided onto ‘Wesley’- Fhb1 backgrounds. Mol Breeding 35:6. https://doi.org/10.1007/s11032-015-0200-1
Article Google Scholar
Federal Department of Agriculture [FDA] (2018). Guidance for Industry and FDA: Advisory Levels for Deoxynivalenol (DON) in Finished Wheat Products for Human Consumption and Grains and Grain By-Products used for Animal Feed. Retrieved from: https://www.fda.gov/Food/GuidanceRegulation/GuidanceDocumentsRegulatoryInformation/ChemicalContaminantsMetalsNaturalToxinsPesticides/ucm120184.htm. Accessed 06 Dec 2018
Giancaspro A, Giove SL, Zito D, Blanco A, Gadaleta A (2016) Mapping QTL for Fusarium head blight resistance in an interspecific wheat population. Front Plant Sci 7:1381. https://doi.org/10.3389/fpls.2016.01381
Article PubMed PubMed Central Google Scholar
He X, Lillemo M, Shi J, Wu J, Bjørnstad Å, Belova T, Dreisigacker S, Duveiller E, Singh P (2016) QTL characterization of Fusarium head blight resistance in CIMMYT bread wheat line Soru#1. PLoS One 11(6):e0158052. https://doi.org/10.1371/journal.pone.0158052
CAS Article PubMed PubMed Central Google Scholar
Holland JB, Nyquist WE, Cervantes-Martinez CT (2002) Estimating and interpreting heritability for plant breeding: an update. Plant Breed Rev 22:109–112. https://doi.org/10.1002/9780470650202.ch2
Article Google Scholar
Holzapfel J, Voss HH, Miedaner T, Korzun V, Haberle J, Schweizer G, Mohler V, Zimmermann G, Hartl L (2008) Inheritance of resistance to Fusarium head blight in three European winter wheat populations. Theor Appl Genet 117:1119–1128. https://doi.org/10.1007/s00122-008-0850-z
Article PubMed Google Scholar
Islam S, Brown-Guedira G, Van Sanford D, Ohm H, Dong Y, Mckendry AL (2016) Novel QTL associated with the Fusarium head blight resistance in Truman soft red winter wheat. Euphytica 207(3):571–592. https://doi.org/10.1007/s10681-015-1550-9
CAS Article Google Scholar
IWGSC - The International Wheat Genome Sequencing Consortium (2018) Shifting the limits in wheat research and breeding using a fully annotated reference genome. Science 361(6403):eaar7191. https://doi.org/10.1126/science.aar7191
Jia H, Zhou J, Xue S, Li G, Yan H, Ran C, Zhang Y, Shi J, Jia L, Wang X, Luo J, Ma Z (2018) A journey to understand wheat Fusarium head blight resistance in the Chinese wheat landrace Wangshuibai. Crop J 6:48–59. https://doi.org/10.1016/j.cj.2017.09.006
Article Google Scholar
Jin F, Zhang D, Bockus W, Baenziger PS, Carver B, Bai G (2013) Fusarium head blight resistance in U.S. wheat cultivars and elite breeding lines. Crop Sci 53:2006–2013. https://doi.org/10.2135/cropsci2012.09.0531
Article Google Scholar
Jordan WK, Wang S, Lun Y, Gardiner LJ, MacLachlan R, Hucl P, Wiebe K, Wong D, Forrest KL, Sharpe AG, Sidebottom CHD, Hall N, Toomajian C, Close T, Dubcovsky J, Akhunova A, Talbert L, Bansal UK, Bariana HS, Hayden MJ, Pozniak C, Jeddeloh J, Hall A, Akhunov E (2015) A haplotype map of allohexaploid wheat reveals distinct patterns of selection on homologous genomes. Genome Biol 16:1–18. https://doi.org/10.1186/s13059-015-0606-4
Article Google Scholar
Kansas Ag Statistics – USDA (2017) Kansas wheat varieties. Online publication available at: https://www.nass.usda.gov/Statistics_by_State/Kansas/Publications/Crops/Wheat_Varieities/KS_whtvar17.pdf
Langmead B, Salzberg S (2012) Fast gapped-read alignment with Bowtie 2. Nat Methods 9:357–359. https://doi.org/10.1038/nmeth.1923
CAS Article PubMed PubMed Central Google Scholar
Li T, Bai G, Wu S, Gu S (2012) Quantitative trait loci for resistance to Fusarium head blight in the Chinese wheat landrace Huangfangzhu. Euphytica 185:93–102. https://doi.org/10.1007/s10681-012-0631-2
Article Google Scholar
Liu S, Abate ZA, Lu H, Musket T, Davis GL, McKendry AL (2007) QTL associated with Fusarium head blight resistance in the soft red wheat Ernie. Theor Appl Genet 115:417–427. https://doi.org/10.1007/s00122-007-0577-2
CAS Article PubMed Google Scholar
Liu S, Hall MD, Griffey CA, McKendry AL (2009) Meta-analysis of QTL associated with Fusarium head blight in wheat. Crop Sci 49:1955–1968. https://doi.org/10.2135/cropsci2009.03.0115
CAS Article Google Scholar
Liu S, Griffey CA, Hall MD, McKendry AL, Chen J, Brooks WS, Brown-Guedira G, Van Sanford D, Schmale DG (2013) Molecular characterization of field resistance to Fusarium head blight in two US soft red winter wheat cultivars. Theor Appl Genet 126:2485–2498. https://doi.org/10.1007/s00122-013-2149-y
CAS Article PubMed PubMed Central Google Scholar
Liu H, Niu Y, Gonzalez-Portilla PJ, Zhou H, Wang L, Zuo T, Qin C, Tai S, Jansen C, Shen Y, Lin H, Lee M, Ware D, Zhang Z, Lübberstedt T, Pan G (2015) An ultra-high-density map as a community resource for discerning the genetic basis of quantitative traits in maize. BMC Genomics 16:1078. https://doi.org/10.1186/s12864-015-2242-5
CAS Article PubMed PubMed Central Google Scholar
Madden LV, Hughes G, Van den Bosch F (2007) The study of plant disease epidemics. The American Phytopathological Society, APS Press, St. Paul, Minnesota
Google Scholar
Mesterhazy A (1995) Type and components of resistance to Fusarium head blight of wheat. Plant Breed 114(5):377–386. https://doi.org/10.1111/j.1439-0523.1995.tb00816.x
Article Google Scholar
Miedaner T, Wilde F, Steiner B, Buerstmayr H, Korzun V, Ebmeyer E (2006) Stacking quantitative trait loci (QTL) for Fusarium head blight resistance from non-adapted sources in a European elite spring wheat background and assessing their effects on deoxynivalenol (DON) content and disease severity. Theor Appl Genet 112:562–569. https://doi.org/10.1007/s00122-005-0163-4
CAS Article PubMed Google Scholar
Muhovski Y, Jacquemin JM, Batoko H (2014) Identification and differential induction of ABCG transporter genes in wheat cultivars challenged by a deoxynivalenol-producing Fusarium graminearum strain. Mol Biol Rep 41:6181–6194. https://doi.org/10.1007/s11033-014-3497-7
CAS Article PubMed Google Scholar
Nganje WE, Kaitibie S, Wilson WW, Leistritz FL, Bangsund DA (2004) Economic impacts of Fusarium head blight in wheat and barley: 1993–2001. Agribusiness and Applied Economics Report n. 538, North Dakota State University
NPGS – U.S. National Plant Germplasm System (2017) Available online at: https://npgsweb.ars-grin.gov/gringlobal/search.aspx
Peiris KHS, Pumphrey MO, Dong Y, Maghirang EB, Berzonsky W, Dowell FE (2010) Near-infrared spectroscopic method for identification of Fusarium head blight damage and prediction of deoxynivalenol in single wheat kernels. Cereal Chem 87:511–517. https://doi.org/10.1094/CCHEM-01-10-0006
CAS Article Google Scholar
Peiris KHS, Dong Y, Bockus WW, Dowell FE (2014) Single-kernel NIR analysis for evaluating wheat samples for Fusarium head blight resistance. Cereal Chem 91:35–40. https://doi.org/10.1094/CCHEM-11-12-0157-R
CAS Article Google Scholar
Petersen S, Lyerly JH, Maloney PV, Brown-Guedira G, Cowger C, Costa JM, Dong Y, Murphy JP (2016) Mapping of Fusarium head blight resistance quantitative trait loci in winter wheat cultivar NC-Neuse. Crop Sci 56:1473–1483. https://doi.org/10.2135/cropsci2015.05.0312
CAS Article Google Scholar
Petersen S, Lyerly JH, McKendry AL, Islam MS, Brown-Guedira G, Cowger C, Dong Y, Murphy JP (2017) Validation of Fusarium head blight resistance QTL in US winter wheat. Crop Sci 57:1–12. https://doi.org/10.2135/cropsci2015.07.0415
CAS Article Google Scholar
Poland JA, Brown PJ, Sorrells ME, Jannink JL (2012) Development of high-density genetic maps for barley and wheat using a novel two-enzyme genotyping-by-sequencing approach. PLoS One 7(2):e32253. https://doi.org/10.1371/journal.pone.0032253
CAS Article PubMed PubMed Central Google Scholar
RStudio Team (2017) RStudio: integrated development for R. RStudio, Inc., Boston, MA, URL http://www.rstudio.com/
SAS Institute Inc (2011) Base SAS® 9.3 Procedures Guide. SAS Institute Inc, Cary, NC
Google Scholar
Semagn K, Skinnes H, Bjørnstad Å, Marøy AG, Tarkegne Y (2007) Quantitative trait loci controlling Fusarium head blight resistance and low deoxynivalenol content in hexaploid wheat population from Arina and NK93604. Crop Sci 47:294–303. https://doi.org/10.2135/cropsci2006.02.0095
CAS Article Google Scholar
Sneller C, Guttieri M, Paul P, Costa J, Jackwood R (2012) Variation for resistance to kernel infection and toxin accumulation in winter wheat infected with Fusarium graminearum. Phytopathology 102(3):306–314. https://doi.org/10.1094/PHYTO-05-11-0143
CAS Article PubMed Google Scholar
Sun J, Ohm HW, Poland JA, Williams CE (2016) Mapping four quantitative trait loci associated with type I Fusarium head blight resistance in winter wheat ‘INW0412’. Crop Sci 56:1163–1172. https://doi.org/10.2135/cropsci2015.06.0390
CAS Article Google Scholar
Tukey JW (1949) Comparing individual means in the analysis of variance. Biometrics 5(2):99–114
CAS Article Google Scholar
Van Ooijen JW (2006) JoinMap 4: Software for the calculation of genetic linkage maps in experimental populations. Kyazma BV, Wageningen
Google Scholar
Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTL. J Hered 93:77–78 Available at http://www.joinmap.nl
CAS Article Google Scholar
Wang J, Luo MC, Chen Z, You FM, Wei Y, Zheng Y, Dvorak J (2013) Aegilops tauschii single nucleotide polymorphisms shed light on the origins of wheat D-genome genetic diversity and pinpoint the geographic origin of hexaploid wheat. New Phytol 198:925–937. https://doi.org/10.1111/nph.12164
CAS Article PubMed Google Scholar
Xue S, Li G, Jia H, Xu F, Lin F, Tang M, Wang Y, An X, Xu H, Zhang L, Kong Z, Ma Z (2010) Fine mapping Fhb4, a major QTL conditioning resistance to Fusarium infection in bread wheat (Triticum aestivum L.). Theor Appl Genet 121:147–156. https://doi.org/10.1007/s00122-010-1298-5
Article PubMed Google Scholar
Xue S, Xu F, Tang M, Zhou Y, Li G, An X, Lin F, Xu H, Jia H, Zhang L, Kong Z, Ma Z (2011) Precise mapping Fhb5, a major QTL conditioning resistance to Fusarium infection in bread wheat (Triticum aestivum L.). Theor Appl Genet 123:1055–1063. https://doi.org/10.1007/s00122-011-1647-z
Article PubMed Google Scholar
Yang Z, Gilbert J, Somers D, Fedak G, Procunier J, McKenzie I (2003) Marker assisted selection of Fusarium head blight resistance genes in two doubled haploid populations of wheat. Mol Breed 12:309–317. https://doi.org/10.1023/B:MOLB.0000006834.44201.48
CAS Article Google Scholar
Yi X, Cheng J, Jiang Z, Hu W, Bie T, Gao D, Li D, Wu R, Li Y, Chen S, Cheng X, Liu J, Zhang Y, Cheng S (2018) Genetic analysis of Fusarium head blight resistance in CIMMYT bread wheat line C615 using traditional and conditional QTL mapping. Front Plant Sci 9:573. https://doi.org/10.3389/fpls.2018.00573
Article PubMed PubMed Central Google Scholar

Download references

Acknowledgements

We are also grateful for the field and lab support provided by Mark Davis, Angela Matthews, Shuangye Wu, Andrew Auld, and Shaun Winnie.

Author contribution statement

CLS designed the study, evaluated the mapping population, performed QTL mapping analysis, and prepared the manuscript. AF and MC developed the mapping population, assisted with field evaluations, and revised the manuscript. JP contributed with genotyping-by-sequencing data. FD and KP assisted with single-kernel near-infrared spectroscopy analysis. All authors read and approved the final version of this manuscript.

Funding

The authors thank the Kansas Wheat Alliance, the Kansas Wheat Commission, and the Kansas State Wheat Breeding Program for supporting this research through funding. This work was also partly funded by the US Wheat & Barley Scab Initiative.

Author information

Authors and Affiliations

Department of Agronomy, 2002 Throckmorton Plant Sciences Center, Kansas State University, 1712 Claflin Rd, Manhattan, KS, 66506, USA
Cristiano Lemes da Silva, Allan Fritz, Marshall Clinesmith & Kamaranga Peiris
Department of Plant Pathology, 4024 Throckmorton Plant Sciences Center, Kansas State University, 1712 Claflin Rd, Manhattan, KS, 66506, USA
Jesse Poland
USDA, Agricultural Research Service, Center for Grain and Animal Health Research, 1515 College Avenue, Manhattan, KS, 66502, USA
Floyd Dowell

Authors

Cristiano Lemes da Silva
View author publications
You can also search for this author in PubMed Google Scholar
Allan Fritz
View author publications
You can also search for this author in PubMed Google Scholar
Marshall Clinesmith
View author publications
You can also search for this author in PubMed Google Scholar
Jesse Poland
View author publications
You can also search for this author in PubMed Google Scholar
Floyd Dowell
View author publications
You can also search for this author in PubMed Google Scholar
Kamaranga Peiris
View author publications
You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Cristiano Lemes da Silva or Allan Fritz.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Disclaimer

Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Key message

For the first time, DON data estimated with single-kernel near-infrared technology allowed mapping of a large-effect QTL associated with low DON accumulation in wheat. Four QTL associated with native FHB resistance were identified in the winter wheat variety ‘Everest’. The major QTL, Qksu-fhbE-5AS, explained 13% of the DON variation in the 2-year average of the study.

Electronic supplementary material

ESM 1

(DOCX 1657 kb)

ESM 2

(XLSX 5.3 mb)

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lemes da Silva, C., Fritz, A., Clinesmith, M. et al. QTL mapping of Fusarium head blight resistance and deoxynivalenol accumulation in the Kansas wheat variety ‘Everest’. Mol Breeding 39, 35 (2019). https://doi.org/10.1007/s11032-019-0937-z

Download citation

Received: 30 October 2018
Accepted: 28 January 2019
Published: 16 February 2019
DOI: https://doi.org/10.1007/s11032-019-0937-z

Keywords

Fusarium head blight
FHB resistance
Deoxynivalenol
QTL mapping
Genotyping-by-sequencing
Near-infrared spectroscopy