QTL mapping of Fusarium head blight resistance and deoxynivalenol accumulation in the Kansas wheat variety ‘Everest’
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Fusarium head blight (FHB) reduces grain yield and accumulates mycotoxins in wheat kernels and flour and thus compromise the end-use quality. In this study, quantitative trait loci (QTL) associated with components of FHB resistance were mapped in the Kansas wheat variety Everest. A doubled haploid (DH) population with 176 lines was developed from a cross between Everest and WB-Cedar, which are moderately resistant and moderately susceptible to FHB, respectively. Two-year field evaluations were conducted using a randomized complete block design with three replications. Deoxynivalenol (DON) accumulation and Fusarium-damaged kernels (FDK) were estimated using a single-kernel near-infrared (SKNIR) system. Four QTL from Everest were identified on 1BS, 3DS, 4BL, and 5AS, indicating that this variety is a source of native FHB resistance conditioned by multiple loci. A large-effect QTL (Qksu-fhbE-5AS) from Everest explained up to 13% of the DON variation in the 2-year experiment average. A single QTL from WB-Cedar, Qksu.fhbC-1AS, was identified contributing FHB resistance. QTL grouping analysis showed that individuals containing multiple QTL were significantly more resistant, indicating that QTL pyramiding enhances FHB resistance. A 36% reduction in DON content was observed in DH lines carrying all mapped QTL for DON accumulation. Further validation of the QTL mapped here will allow breeding programs to increase FHB resistance in other genetic backgrounds. This is the first study that identified QTL associated with low DON accumulation in multiple years of field experiment using the SKNIR technology, supporting the use of this methodology in future genetics and breeding studies.
KeywordsFusarium head blight FHB resistance Deoxynivalenol QTL mapping Genotyping-by-sequencing Near-infrared spectroscopy
We are also grateful for the field and lab support provided by Mark Davis, Angela Matthews, Shuangye Wu, Andrew Auld, and Shaun Winnie.
Author contribution statement
CLS designed the study, evaluated the mapping population, performed QTL mapping analysis, and prepared the manuscript. AF and MC developed the mapping population, assisted with field evaluations, and revised the manuscript. JP contributed with genotyping-by-sequencing data. FD and KP assisted with single-kernel near-infrared spectroscopy analysis. All authors read and approved the final version of this manuscript.
The authors thank the Kansas Wheat Alliance, the Kansas Wheat Commission, and the Kansas State Wheat Breeding Program for supporting this research through funding. This work was also partly funded by the US Wheat & Barley Scab Initiative.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer.
- Bernardo, R (2009) Should maize doubled haploids be induced among F1 or F2 plants? Theor Appl Genet (2009) 119: 255–262. doi: https://doi.org/10.1007/s00122-009-1034-1
- Bockus WW, Appel JA, De Wolf ED, Todd TC, Davis MA, Fritz AK (2015) Impact of wheat cultivar Everest on yield loss in Kansas from Fusarium head blight during 2015. Proceedings of the 2015 National Fusarium Head Blight Forum. St Louis MOGoogle Scholar
- Buerstmayr M, Steiner B, Wagner C, Schwarz P, Brugger K, Barabaschi D, Volante A, Valè G, Cattivelli L, Buerstmayr H (2017) High-resolution mapping of the pericentromeric region on wheat chromosome arm 5AS harboring the Fusarium head blight resistance QTLQfhs.ifa-5A. Plant Biotechnol J 16:1046–1056. https://doi.org/10.1111/pbi.12850 CrossRefPubMedPubMedCentralGoogle Scholar
- Burt C, Steed A, Gosman N, Lemmens M, Bird N, Ramirez-Gonzalez R, Holdgate S, Nicholson P (2015) Mapping a type 1 FHB resistance on chromosome 4AS of Triticum macha and deployment in combination with two type 2 resistances. Theor Appl Genet 128(9):1725–1738. https://doi.org/10.1007/s00122-015-2542-9 CrossRefPubMedPubMedCentralGoogle Scholar
- Cai J (2016) Meta-analysis of QTL for Fusarium head blight resistance in Chinese wheat landraces using genotyping by sequencing. Dissertation, Kansas State UniversityGoogle Scholar
- Clinesmith M (2016) Genetic mapping of QTL for Fusarium head blight resistance in winter wheat cultivars Art and Everest. Master Thesis, Kansas State UniversityGoogle Scholar
- Costa JM, Bockelman HE, Brown-Guedira G, Cambron SE, Chen X, Cooper A, Cowger C, Dong Y, Grybauskas A, Jin Y, Kolmer J, Murphy JP, Sneller C, Souza E (2010) Registration of the soft red winter wheat germplasm MD01W233–06–1 resistant to Fusarium head blight. J Plant Reg 4:255–260. https://doi.org/10.3198/jpr2010.01.0034crg CrossRefGoogle Scholar
- De Wolf ED, Bockus WW, Whitworth RJ (2015) Wheat variety disease and insect rating 2015. Kansas State University, Manhattan, KSGoogle Scholar
- Draeger R, Gosman N, Steed A, Chandler E, Thomsett M, Schondelmaier J, Buerstmayr H, Lemmens M, Schmolke M, Mesterhazy A, Nicholson P (2007) Identification of QTL for resistance to Fusarium head blight, DON accumulation and associated traits in the winter wheat variety Arina. Theor Appl Genet 115:617–625. https://doi.org/10.1007/s00122-007-0592-3 CrossRefPubMedGoogle Scholar
- Eckard J, Gonzalez-Hernandez J, Caffe M, Berzonsky W, Bockus W, Marais G, Baenziger P (2015) Native Fusarium head blight resistance from winter wheat cultivars ‘Lyman,’ ‘Overland,’ ‘Ernie,’ and ‘Freedom’ mapped and pyramided onto ‘Wesley’- Fhb1 backgrounds. Mol Breeding 35:6. https://doi.org/10.1007/s11032-015-0200-1 CrossRefGoogle Scholar
- Federal Department of Agriculture [FDA] (2018). Guidance for Industry and FDA: Advisory Levels for Deoxynivalenol (DON) in Finished Wheat Products for Human Consumption and Grains and Grain By-Products used for Animal Feed. Retrieved from: https://www.fda.gov/Food/GuidanceRegulation/GuidanceDocumentsRegulatoryInformation/ChemicalContaminantsMetalsNaturalToxinsPesticides/ucm120184.htm. Accessed 06 Dec 2018
- Holzapfel J, Voss HH, Miedaner T, Korzun V, Haberle J, Schweizer G, Mohler V, Zimmermann G, Hartl L (2008) Inheritance of resistance to Fusarium head blight in three European winter wheat populations. Theor Appl Genet 117:1119–1128. https://doi.org/10.1007/s00122-008-0850-z CrossRefPubMedGoogle Scholar
- IWGSC - The International Wheat Genome Sequencing Consortium (2018) Shifting the limits in wheat research and breeding using a fully annotated reference genome. Science 361(6403):eaar7191. https://doi.org/10.1126/science.aar7191
- Jordan WK, Wang S, Lun Y, Gardiner LJ, MacLachlan R, Hucl P, Wiebe K, Wong D, Forrest KL, Sharpe AG, Sidebottom CHD, Hall N, Toomajian C, Close T, Dubcovsky J, Akhunova A, Talbert L, Bansal UK, Bariana HS, Hayden MJ, Pozniak C, Jeddeloh J, Hall A, Akhunov E (2015) A haplotype map of allohexaploid wheat reveals distinct patterns of selection on homologous genomes. Genome Biol 16:1–18. https://doi.org/10.1186/s13059-015-0606-4 CrossRefGoogle Scholar
- Kansas Ag Statistics – USDA (2017) Kansas wheat varieties. Online publication available at: https://www.nass.usda.gov/Statistics_by_State/Kansas/Publications/Crops/Wheat_Varieities/KS_whtvar17.pdf
- Liu S, Griffey CA, Hall MD, McKendry AL, Chen J, Brooks WS, Brown-Guedira G, Van Sanford D, Schmale DG (2013) Molecular characterization of field resistance to Fusarium head blight in two US soft red winter wheat cultivars. Theor Appl Genet 126:2485–2498. https://doi.org/10.1007/s00122-013-2149-y CrossRefPubMedPubMedCentralGoogle Scholar
- Liu H, Niu Y, Gonzalez-Portilla PJ, Zhou H, Wang L, Zuo T, Qin C, Tai S, Jansen C, Shen Y, Lin H, Lee M, Ware D, Zhang Z, Lübberstedt T, Pan G (2015) An ultra-high-density map as a community resource for discerning the genetic basis of quantitative traits in maize. BMC Genomics 16:1078. https://doi.org/10.1186/s12864-015-2242-5 CrossRefPubMedPubMedCentralGoogle Scholar
- Madden LV, Hughes G, Van den Bosch F (2007) The study of plant disease epidemics. The American Phytopathological Society, APS Press, St. Paul, MinnesotaGoogle Scholar
- Mesterhazy A (1995) Type and components of resistance to Fusarium head blight of wheat. Plant Breed 114(5):377–386. https://doi.org/10.1111/j.1439-0523.1995.tb00816.x CrossRefGoogle Scholar
- Miedaner T, Wilde F, Steiner B, Buerstmayr H, Korzun V, Ebmeyer E (2006) Stacking quantitative trait loci (QTL) for Fusarium head blight resistance from non-adapted sources in a European elite spring wheat background and assessing their effects on deoxynivalenol (DON) content and disease severity. Theor Appl Genet 112:562–569. https://doi.org/10.1007/s00122-005-0163-4 CrossRefPubMedGoogle Scholar
- Nganje WE, Kaitibie S, Wilson WW, Leistritz FL, Bangsund DA (2004) Economic impacts of Fusarium head blight in wheat and barley: 1993–2001. Agribusiness and Applied Economics Report n. 538, North Dakota State UniversityGoogle Scholar
- NPGS – U.S. National Plant Germplasm System (2017) Available online at: https://npgsweb.ars-grin.gov/gringlobal/search.aspx
- Peiris KHS, Pumphrey MO, Dong Y, Maghirang EB, Berzonsky W, Dowell FE (2010) Near-infrared spectroscopic method for identification of Fusarium head blight damage and prediction of deoxynivalenol in single wheat kernels. Cereal Chem 87:511–517. https://doi.org/10.1094/CCHEM-01-10-0006 CrossRefGoogle Scholar
- RStudio Team (2017) RStudio: integrated development for R. RStudio, Inc., Boston, MA, URL http://www.rstudio.com/
- SAS Institute Inc (2011) Base SAS® 9.3 Procedures Guide. SAS Institute Inc, Cary, NCGoogle Scholar
- Semagn K, Skinnes H, Bjørnstad Å, Marøy AG, Tarkegne Y (2007) Quantitative trait loci controlling Fusarium head blight resistance and low deoxynivalenol content in hexaploid wheat population from Arina and NK93604. Crop Sci 47:294–303. https://doi.org/10.2135/cropsci2006.02.0095 CrossRefGoogle Scholar
- Van Ooijen JW (2006) JoinMap 4: Software for the calculation of genetic linkage maps in experimental populations. Kyazma BV, WageningenGoogle Scholar
- Wang J, Luo MC, Chen Z, You FM, Wei Y, Zheng Y, Dvorak J (2013) Aegilops tauschii single nucleotide polymorphisms shed light on the origins of wheat D-genome genetic diversity and pinpoint the geographic origin of hexaploid wheat. New Phytol 198:925–937. https://doi.org/10.1111/nph.12164 CrossRefPubMedGoogle Scholar
- Xue S, Li G, Jia H, Xu F, Lin F, Tang M, Wang Y, An X, Xu H, Zhang L, Kong Z, Ma Z (2010) Fine mapping Fhb4, a major QTL conditioning resistance to Fusarium infection in bread wheat (Triticum aestivum L.). Theor Appl Genet 121:147–156. https://doi.org/10.1007/s00122-010-1298-5 CrossRefPubMedGoogle Scholar
- Xue S, Xu F, Tang M, Zhou Y, Li G, An X, Lin F, Xu H, Jia H, Zhang L, Kong Z, Ma Z (2011) Precise mapping Fhb5, a major QTL conditioning resistance to Fusarium infection in bread wheat (Triticum aestivum L.). Theor Appl Genet 123:1055–1063. https://doi.org/10.1007/s00122-011-1647-z CrossRefPubMedGoogle Scholar
- Yang Z, Gilbert J, Somers D, Fedak G, Procunier J, McKenzie I (2003) Marker assisted selection of Fusarium head blight resistance genes in two doubled haploid populations of wheat. Mol Breed 12:309–317. https://doi.org/10.1023/B:MOLB.0000006834.44201.48 CrossRefGoogle Scholar
- Yi X, Cheng J, Jiang Z, Hu W, Bie T, Gao D, Li D, Wu R, Li Y, Chen S, Cheng X, Liu J, Zhang Y, Cheng S (2018) Genetic analysis of Fusarium head blight resistance in CIMMYT bread wheat line C615 using traditional and conditional QTL mapping. Front Plant Sci 9:573. https://doi.org/10.3389/fpls.2018.00573 CrossRefPubMedPubMedCentralGoogle Scholar