Identification and mapping of net form of net blotch resistance in South African barley
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Net form of net blotch (NFNB) caused by the fungus Pyrenophora teres f. teres is an economically important foliar disease of barley (Hordeum vulgare) in southern and eastern Africa. Little attention has been given to disease resistance breeding, and knowledge about the presence of NFNB resistance in breeding lines is limited. Deploying resistance into varieties used in this region is important for future control of the disease. We have identified NFNB disease resistance in existing South African breeders’ lines and have mapped the resistance in line UVC8. Six different trials, three conducted in South Africa and another three in Australia, were used to identify resistance QTL. A major QTL was identified on chromosome 6H having a LOD score of 40.5 and 55% of the phenotypic variance explained. Kompetitive Allele Specific PCR (KASP™) markers were designed for this QTL region. These and microsatellite markers can now be used to routinely select for NFNB resistance.
KeywordsHordeum vulgare Net form of net blotch Quantitative trait loci KASP™ markers
We would like to thank the South African Winter Cereal Trust and the South African National Research Foundation for funding this project and Judy McIlroy, Janet Barsby, Lizaan Rademeyer, and Debbie Snyman for their technical support. We would also like to thank Dr. Kelvin Khoo for his help with the initial KAPS™ marker development.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interests.
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