Temperature-dependent QTLs in indica alleles for improving grain quality in rice: increased prominence of QTLs responsible for reduced chalkiness under high-temperature conditions
Quantitative trait loci (QTLs) for the apparent quality of brown rice under high temperatures during ripening were analyzed using chromosomal segment substitution lines. Segments from the indica cultivar Habataki were substituted into a japonica cultivar with a Sasanishiki background. We found the following two QTLs for increasing grain quality in the Habataki allele on chromosome 3: (1) qTW3-2, located near the marker RM14702, decreased the percentage of total white immature (TWI) grains, and (2) qRG3-2, located near RM3766, increased the percentage of regular grains. The effects of these two QTLs were more obvious under high-temperature ripening conditions; hence, these loci are considered QTLs not only for reducing TWI grains but also for increasing high-temperature tolerance. Additionally, we found two QTLs, i.e., qTW3-1 and qRG3-1, responsible for reduced grain quality near RM14314 on chromosome 3. Although the QTL for narrow grains in the Habataki allele qNG3 was genetically linked to qTW3-2, the effect was only slightly significant, and the length/width ratio of qNG3-carrying grains was within the range observed in widely grown japonica cultivars. Incorporating the Habataki region, including qRG3-2 and qTW3-2 but not qTW3-1 and qRG3-1, in addition to previously reported grain quality QTLs in breeding japonica cultivars will improve high-temperature tolerance and grain quality.
KeywordsHigh-temperature tolerance QTL Grain quality Rice (Oryza sativa L.)
We are grateful to Mr. Tokuya Genba, Mr. Hiroyuki Nakagawa, Mr. Tuyoshi Kotake, Mr. Masanori Ichihashi, Mr. Shinobu Yuminamochi, Mr. Koushi Yazaki, Mr. Susumu Saitou, Mr. Ken-ichi Koide, Ms. Keiko Nozaki, Ms. Setsuko Hayashi, and Ms. Kiiko Takatsuto for their excellent technical assistance.
This study was supported by Japan Society for the Promotion of Science KAKENHI grants 24580030 and 15K0280.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
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