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Identification of the male parent of superior half-sib Populus tomentosa individuals based on SSR markers

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Abstract

When hundreds of cross-pollinated trees planted in The National Gene Bank of Populus tomentosa blossom and bear fruit, in theory, half-sib progeny should originate from hundreds of combinations. Paternity testing was performed on the superior half-sib progeny with outstanding performance using microsatellite markers, a process that can be used to rapidly screen for superior cross combinations and significantly improve the efficiency and effectiveness of genetic improvements. Based on the comprehensive evaluation of the eight main growth traits of 2292 plants of 30 half-sib families, 77 plants consisting of 43 triploid and 34 diploid plants were selected as superior. We used 14 pairs of highly polymorphic SSR primers to identify the male parents of the 77 superior plants. Our results identified 29 male trees as the pollen parents of 59 superior plants from 49 cross combinations. The probabilities of 2-14, 3-48-1, 1903, 2-1, 4234, 5099, and 4114 as male parents were significantly higher than those of other males, suggesting that these male parents have potential for applications in breeding strategies of P. tomentosa. Thus, this study used SSR molecular markers to identify the male parents of superior individuals in a P. tomentosa population, laying a foundation for the application of maternal efficiency and construction of optimal hybrid parent groups.

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Acknowledgments

The authors would like to thank the staff of Guanxian nursery in Shandong Province, China, for providing their experimental field. We were also grateful to the anonymous reviewers and editors for their constructive comments. This work was supported by National Natural Science Foundation of China (31530012): The Program of the Co-Construction with Beijing Municipal Commission of Education of China.

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Authors and Affiliations

  1. Beijing Advanced Innovation Center For Tree Breeding By Molecular Design, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China

    Zhiqiang Han, Peng Gao, Xining Geng, Kang Du & Xiangyang Kang

  2. Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Laboratory of Urban and Rural Ecological Environment, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China

    Zhiqiang Han, Peng Gao, Xining Geng, Kang Du & Xiangyang Kang

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  1. Zhiqiang Han
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  2. Peng Gao
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  4. Kang Du
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Correspondence to Xiangyang Kang.

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The experiments reported in this study comply with the current laws of China.

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Han, Z., Gao, P., Geng, X. et al. Identification of the male parent of superior half-sib Populus tomentosa individuals based on SSR markers. Mol Breeding 37, 155 (2017). https://doi.org/10.1007/s11032-017-0754-1

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