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Identifying aluminum tolerance in rice with a molecular marker

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Abstract

Improving rice aluminum (Al) tolerance in acid soils is highly significant to rice production. So far, no molecular marker which can efficiently identify rice Al tolerance has been developed. In this study, Art1, a primer specific for the ART1 gene was designed to study whether some natural variations exist in ART1 between Al tolerant and sensitive varieties. The results showed that two Al tolerant varieties had identical nucleotides and amino acids, while three Al sensitive varieties had the same nucleotides and amino acids differing from the two Al tolerant varieties. The same results were discovered within 150 rice varieties of Ting’s core collection. Art1 could be used to judge rice Al tolerance.

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Acknowledgements

We are grateful to Dr. Jinquan Li from Max Planck Institute for Plant Breeding Research, Dr. Xiangdong Liu from South China Agricultural University and Dr. Chaofeng Huang from Shanghai Center for Plant Stress Biology, and Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences. This work was supported by National Natural Science Foundation of China, grant 31701401, Agricultural Sciences and Technologies Innovation Program of Chinese Academy of Agricultural Sciences, and a fund in Zhejiang Province for public welfare (2016C32094).

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Author notes

  1. Peng Zhang and Kaizhen Zhong contributed equally to this study

Authors and Affiliations

  1. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China

    Peng Zhang, Zhengzheng Zhong & Hanhua Tong

  2. School of Agricultural Sciences, Jiangxi Agricultural University, Nanchang, 330045, China

    Kaizhen Zhong

Authors
  1. Peng Zhang
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  2. Kaizhen Zhong
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  3. Zhengzheng Zhong
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  4. Hanhua Tong
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Contributions

Conceived and designed the experiments: PZ and HT. Performed the experiments: PZ and KZ. Analyzed the data: PZ, KZ, ZZ and HT. Contributed reagents/materials/analysis tools: PZ and HT. Wrote the paper: PZ, KZ and HT.

Corresponding authors

Correspondence to Peng Zhang or Hanhua Tong.

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Zhang, P., Zhong, K., Zhong, Z. et al. Identifying aluminum tolerance in rice with a molecular marker. Mol Breeding 37, 156 (2017). https://doi.org/10.1007/s11032-017-0748-z

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  • DOI: https://doi.org/10.1007/s11032-017-0748-z

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