Abstract
Faba bean (Vicia faba L.) is a valuable feed and food crop with potential for development globally as a staple protein crop. Its consumption is limited by the anti-nutritional factors vicine and convicine (v-c) in its seeds. A single gene (vc -) confers the low v-c phenotype in faba bean. Time-consuming and laborious quantitative chemical analysis is currently used in breeding programs to detect v-c concentration. Molecular markers within or linked to the vc - gene could facilitate rapid and cost-effective screening of early generation breeding populations for low v-c concentration. The large and complex faba bean genome has been an impediment to the progress of development of molecular breeding strategies. Here, we report a high-throughput low-cost KASP (Kompetitive Allele Specific PCR) marker for low v-c concentration in faba bean. The KASP assay successfully distinguished low and high v-c lines of faba bean. This marker is a significant and valuable molecular tool for faba bean breeding programs aiming to reduce v-c from faba beans worldwide.
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References
Cappellini MD, Fiorelli G (2008) Glucose-6-phosphate dehydrogenase deficiency. Lancet 371:64–74
Collier HB (1976) The estimation of vicine in faba beans by an ultraviolet spectrophotometric method. Can Instit Food Sci Technol J 9:155–159
Cooper J, Wilson M, Derks M, Smit S, Kunert K, Cullis C, Foyer CH (2017) Enhancing faba bean (Vicia faba L.) genome resources. J Exp Bot 68:1941–1953
Crépon K, Marget P, Peyronnet C, Carrouée B, Arese P, Duc G (2010) Nutritional value of faba bean (Vicia faba L.) seeds for feed and food. Field Crops Res 115:329–339
Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
Duc G, Marget P, Page D, Domoney C (2004) Facile breeding markers to lower contents of vicine and convicine in faba bean seeds and trypsin inhibitors in pea seeds. Proceedings of the fourth international workshop on antinutritional factors in legume seeds and oilseeds, 2004/03/08-10, Toledo (ESP). EEAP, European association for animal production (FRA). In: Musquiz M, Hill GD, Cuadrado MM, Burbano C (eds) Recent advances of research in antinutritional factors in legume seeds and oilseeds. Wageningen Academic Publishers, The Netherlands, pp 281–285 http://www.wageningenacademic.com/doi/book/10.3920/978-90-8686-524-6
Duc G, Sixdenier G, Lila M, Furstoss V (1989) Search of genetic variability for vicine and convicine content in Vicia faba L. A first report of a gene which codes for nearly zero-vicine and zero-convicine contents. Wageningen, The Netherlands (Pbs), pp 305–313
Gutierrez N, Avila CM, Duc G, Marget P, Suso MJ, Moreno MT, Torres AM (2006) CAPs markers to assist selection for low vicine and convicine contents in faba bean (Vicia faba L.). Theor Appl Genet 114:59–66
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
Khamassi K, Ben Jeddi F, Hobbs D, Irigoyen J, Stoddard F, O’Sullivan DM, Jones H (2013) A baseline study of vicine–convicine levels in faba bean (Vicia faba L.) germplasm. Plant Genet Resour 11:250–257
Khazaei H, O’Sullivan DM, Jones H, Pitts N, Sillanpää MJ, Pärssinen P, Manninen O, Stoddard FL (2015a) Flanking SNP markers for vicine-convicine concentration in faba bean (Vicia faba L.). Mol Breed 35:38
Khazaei H, Sing G, Stoddard FL, Bett KE, Vandenberg B (2015b) Faba bean (Vicia faba L.) breeding for key quality traits—past, present and future. NAPIA meeting. 4–6 November 2015. Niagara Falls, Ontario, Canada
Koivunen E, Tuunainen P, Valkonen E, Rossow L, Valaja J (2014) Use of faba beans (Vicia faba L.) in diets of laying hens. Agr Food Sci 23:165–172
Marquardt RR, Frohlich AA (1981) Rapid reversed-phase high-performance liquid chromatographic method for the quantitation of vicine, convicine and related compounds. J Chromatogr 208:373–379
Pulkkinen M, Gautam M, Lampi AM, Ollilainen V, Stoddard FL, Sontag-Strohm T, Piironen V (2015) Determination of vicine and convicine from faba bean with an optimized high-performance liquid chromatographic method. Food Res Int 76:168–177
Purves RW, Khazaei H, Vandenberg A (2018) Quantification of vicine and convicine in faba bean seeds using hydrophilic interaction liquid chromatography. Food Chem 240:1137–1145
Purves RW, Khazaei H, Zhang H, Vandenberg A (2017a) A comparison of analytical methods used to measure antinutritional compounds vicine and convicine in faba bean (Vicia faba L.). In: 65th ASMS conference. Indianapolis, Indiana, USA
Purves RW, Zhang H, Khazaei H, Vandenberg A (2017b) Rapid analysis of medically relevant compounds in faba bean seeds using FAIMS and mass spectrometry. Int J Ion Mobil Spec. https://doi.org/10.1007/s12127-017-0226-7
R Development Core Team (2016) R: a language and environment for statistical computing. R foundation for statistical computing, Vienna, Austria. ISBN 3–900051–07–0. Available: http://www.R-project.org
Ramsay G, Griffiths DW (1996) Accumulation of vicine and convicine in Vicia faba and Vicia narbonensis. Phytochemistry 42:63–67
Ray H, Bock C, Georges F (2015) Faba bean: transcriptome analysis from etiolated seedling and developing seed coat of key cultivars for synthesis of proanthocyanidins, phytate, raffinose family oligosaccharides, vicine, and convicine. Plant Genome 8:1. https://doi.org/10.3835/plantgenome2014.07.002
Sixdenier G, Cassecuelle F, Guillaumin L, Duc G (1996) Rapid spectrophotometric method for reduction of vicine and convicine in faba bean seed. FABIS Newslett 38:42–44
Soltis DE, Soltis PS, Bennett MD, Leitch IJ (2003) Evolution of genome size in the angiosperms. Am J Bot 90:1596–1603
Vilariño M, Métayer JP, Crépon K, Duc G (2009) Effects of varying vicine, convicine and tannin contents of faba bean seeds (Vicia faba L.) on nutritional values for broiler chicken. Animal Feed Sci Technol 150:114–121
Webb A, Cottage A, Wood T, Khamassi K, Hobbs D, Gostkiewicz K, White M, Khazaei H, Ali M, Street D, Duc G, Stoddard F, Maalouf F, Ogbannaya F, Link W, Thomas J, O’Sullivan DM (2016) A SNP-based consensus genetic map for synteny-based trait targeting in faba bean (Vicia faba L.). Plant Biotech J 14:177–185
Acknowledgments
This research was supported by the ADF (Agriculture Development Fund, government of Saskatchewan, Canada); the Saskatchewan Pulse Growers (SPG); the University of Saskatchewan; the University of Helsinki, Finland; and the NORFAB project (Protein for the Northern Hemisphere) funded by Innovation fund, Denmark. The authors would like to thank Brent Barlow, Alison Sackville, Parvaneh Hashemi, Anouk Hendriks, and Mohamad Kazem Rezaei for their kind technical support during the experiments.
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Conceived and designed the experiments: HK, KEB, and AV. Performed the experiments: HK, RWP, and MS. Analyzed the data: HK, RWP, MS, and RS. Contributed reagents/materials/analysis tools: HK, FLS, and AV. Wrote the first draft: HK.
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Key message
We developed and validated a robust molecular marker for high-throughput and cost-effective screening for low vicine-convicine concentration in faba bean.
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Khazaei, H., Purves, R.W., Song, M. et al. Development and validation of a robust, breeder-friendly molecular marker for the vc - locus in faba bean. Mol Breeding 37, 140 (2017). https://doi.org/10.1007/s11032-017-0742-5
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DOI: https://doi.org/10.1007/s11032-017-0742-5