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Expression of the sucrose transporter 3 (HbSUT3) in rubber tree and its relation to latex yield

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Abstract

Sucrose is not only a precursor of rubber biosynthesis, but it also participates in other latex regeneration between repeated tapping. Sucrose transporter (HbSUT) genes play key roles in sinks and sources of the rubber biosynthesis pathway. HbSUT3 was dominant in expression in latex among the SUT member genes. Therefore, the expression level of HbSUT3 in latex is a potential indicator distinguishing between high- and low-yielding clones. The aim of this research was to assess the potential of this gene in selective breeding to improve latex yield, from the correlation of HbSUT3 expression with latex yield. Four high-yielding clones were sampled in this study and compared with the common RRIM 600 clone as the baseline, paired by the field. Among the putative full-length cDNAs of Hevea sucrose transporters available in the NCBI database, only HbSUT3 was detected. The HbSUT3 gene was overexpressed in the four rubber clones relative to the control, and the NK1 clone had the highest expression level. The expression level of HbSUT3 correlated positively with latex yield but negatively with the sucrose content of latex. Gene expression analysis of rubber seedlings indicated that the bark had higher expression of this gene than the leaves, and the levels correlated with latex yields of these clones. These data provided new candidate selection criteria for use in the early selection of high-yielding rubber clones, necessary for rapid cycle breeding programs.

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Acknowledgments

This research was mainly supported by the National Research University Project of Thailand and the Graduate School, Prince of Songkla University. The authors would like to thank Associate Professor Seppo Karrila and the Publication Clinic, Research and Development Office, Prince of Songkla University, for the technical comments and manuscript corrections.

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Authors and Affiliations

  1. Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Auksorn Klaewklad, Korakot Nakkanong & Charassri Nualsri

  2. Surat Thani Rubber Research Center, Tha Chana District, Surat Thani, 84170, Thailand

    Chatchamon Daengkanit Nathaworn

Authors
  1. Auksorn Klaewklad
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  2. Korakot Nakkanong
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  3. Chatchamon Daengkanit Nathaworn
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  4. Charassri Nualsri
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Correspondence to Charassri Nualsri.

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Klaewklad, A., Nakkanong, K., Nathaworn, C.D. et al. Expression of the sucrose transporter 3 (HbSUT3) in rubber tree and its relation to latex yield. Mol Breeding 37, 122 (2017). https://doi.org/10.1007/s11032-017-0694-9

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