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Genetic dissection of large grain shape in rice cultivar ‘Nanyangzhan’ and validation of a grain thickness QTL (qGT3.1) and a grain length QTL (qGL3.4)

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Abstract

Grain shape is an important agronomic trait in rice, which influences the yield and quality. In order to dissect the genetic basis of the large grain shape in ‘Nanyangzhan’, a recombinant inbred line (RIL) population derived from Nanyangzhan (NYZ) and Zhenshan 97B (ZS97) was used to map quantitative trait loci (QTLs) for grain length (GL), width (GW), thickness (GT), length-to-width ratio (LWR) and kilo-grain weight (KGW). A total of 53 QTLs were detected and distributed on 11 chromosomes in 2 years. Among those, QTLs for GW and GL showed a concentrated distribution on chromosome 2 and chromosome 3, respectively. NYZ, the parent with large grain shape, carried 44 alleles showing positive effects on the studied traits. In addition, the near-isogenic lines (NILs) of two novel QTLs, qGT3.1 and qGL3.4, were constructed with the background of ZS97. Results showed that NIL-qGT3.1 NYZ, the NIL carrying homozygous qGT3.1 regions from NYZ, showed an increased value of 0.12 mm in grain thickness on average as compared to NIL-qGT3.1 ZS. Similarly, NIL-qGL3.4 NYZ increased the length of each grain by 0.47 mm on average as compared to NIL-qGL3.4 ZS. Taken together, these results would be of great use in breeding rice cultivars with desirable grain shape.

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Acknowledgements

This work was supported by grants from the National Natural Science Foundation (91435202), the National Program on R&D of Transgenic Plants (2014ZX08009-036B), the National 863 Project (2012AA10A303) and the earmarked fund for the China Agriculture Research System (CARS-01-03) of China.

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Correspondence to Yuqing He.

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Zhao, D., Li, P., Wang, L. et al. Genetic dissection of large grain shape in rice cultivar ‘Nanyangzhan’ and validation of a grain thickness QTL (qGT3.1) and a grain length QTL (qGL3.4). Mol Breeding 37, 42 (2017). https://doi.org/10.1007/s11032-017-0638-4

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  • DOI: https://doi.org/10.1007/s11032-017-0638-4

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