Development of molecular markers for breeding of double flowers in Japanese gentian
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Double flowers are valuable floral traits in most floricultural plants. We recently revealed that a double-flowered mutant of Gentiana scabra was caused by an insertion of a retrotransposable element (Tgs1) into GsAG1, one of the C-class MADS-box genes in gentian. In this study, we developed a PCR-based molecular DNA marker to distinguish double- and single-flower phenotypes at the young seedling stage in Japanese gentian plants. To test the validity of the markers, 17 F2 populations were produced by selfing F1 plants crossed between the double-flower mutant and seven breeding lines. Multiplex PCR demonstrated that the Tgs1 insertion in GsAG1 cosegregated with the double-flower phenotype in two F2 populations, indicating that the PCR-based DNA marker was useful to discriminate between double- and single-flower phenotypes in advance of flowering in Japanese gentian. Given that Japanese gentians lack variation in flower shape and require a long breeding period, the DNA marker developed here will be helpful for efficient breeding of double-flowered cultivars in the future.
KeywordsC-class MADS-box gene Double-flower GsAG1 Japanese gentian Molecular marker
polymerase chain reaction
marker assisted selection
We thank Ms. H. Harumi for technical assistance with PCR and scoring of flower phenotypes in F2 populations. This research was financially supported by the Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry (No. 27030C).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Chen CM, Wei TY, Yeh DM (2012) Morphology and inheritance of double floweredness in Catharanthus roseus. Hortscience 47:1679–1681Google Scholar
- Henry RJ (2012) Molecular Markers in Plants. Wiley-Blackwell. doi: 10.1002/9781118473023
- Hikage T, Kogusuri K, Tanaka-Saito C, Watanabe S, Chiba S, Kume K, Doi H, Saitoh Y, Takahata Y, Tsutsumi K (2011) W14/15 esterase gene haplotype can be a genetic landmark of cultivars and species of the genus Gentiana L. Mol Gen Genomics 285:47–56. doi: 10.1007/s00438-010-0582-z CrossRefGoogle Scholar
- Hughes S (1993) Carnations and pinks: the complete guide. The Crowood Press Ltd, Marlborough, UKGoogle Scholar
- Kodama K (2006). IV-9 Gentian In Japanese Society of Hortuculture Science (ed), Horticulture in Japan 2006. Shoukadoh Publishing, Kyoto 248–253Google Scholar
- Ma N, Chen W, Fan T, Tian Y, Zhang S, Zeng D, Li Y (2015) Low temperature-induced DNA hypermethylation attenuates expression of RhAG, an AGAMOUS homolog, and increases petal number in rose (Rosa hybrida). BMC Plant Biol 15:237. doi: 10.1186/s12870-015-0623-1 CrossRefPubMedPubMedCentralGoogle Scholar
- Nakatsuka T, Saito M, Yamada E, Fujita K, Yamagishi N, Yoshikawa N, Nishihara M (2015) Isolation and characterization of the C-class MADS-box gene involved in the formation of double flowers in Japanese gentian. BMC Plant Biol 15:182. doi: 10.1186/s12870-015-0569-3
- Nakatsuka T, Saito M, Nishihara M (2016) Functional characterization of duplicated B-class MADS-box genes in Japanese gentian. Plant Cell Rep 35:895–904. doi: 10.1007/s00299-015-1930-6
- Nishihara M, Hikage T, Yamada E, Nakatsuka T (2011) A single-base substitution suppresses flower color mutation caused by a novel miniature inverted-repeat transposable element in gentian. Mol Gen Genomics 286:371-382. doi: 10.1007/s00438-011-0652-x
- Nishihara M, Mishiba K, Imamura T, Takahashi H, Nakatsuka T (2015) Molecular breeding of Japanese gentians - Applications of genetic transformation, metabolome analyses, and genetic markers. The Gentianaceae : Volume 2 - Biotechnology and Applications, Springer. doi: 10.1007/978–3–642-54102-5_10
- Staub JEF, Serque FC, Gupta M (1996) Genetic markers, map construction, and their application in plant breeding. Hortscience 31:729–741Google Scholar