Molecular Breeding

, 36:160 | Cite as

Introgression of acylsugar chemistry QTL modifies the composition and structure of acylsugars produced by high-accumulating tomato lines

  • John R. Smeda
  • Anthony L. Schilmiller
  • Robert L. Last
  • Martha A. Mutschler
Article

Abstract

Acylsugars are important insect defense compounds produced at high levels by glandular trichomes of the wild tomato, Solanum pennellii. The ability to produce acylsugars at elevated levels was bred into the tomato line CU071026. This study utilized a marker-assisted backcross approach to individually introgress into CU071026 and to fine map the three quantitative trait loci (QTL) fatty acid 5 (FA5QTL), fatty acid 7 (FA7QTL), and fatty acid 8 (FA8QTL), which were previously associated with changes in acylsugar chemistry. Additional breeding with and fine mapping the previously introgressed QTL, fatty acid 2 (FA2QTL), was also conducted. The effect of these four QTL on acylsugar quality and quantity in the presence of the five introgressions of CU071026 was evaluated. Incorporation of the QTL altered acylsugar chemotype by modulating the length, orientation, and/or relative proportion of fatty acid acyl groups. The resulting quantities of acylsugar produced in most of the new lines were similar to that of CU071026; however, introgression of FA5QTL reduced acylsugar levels. The acylsugar lines containing each QTL were characterized for acylsugar level, trichome abundance, and acylsugar chemistry through gas chromatography/mass spectrometry and liquid chromatography/mass spectrometry. The novel acylsugar chemotype lines created can contribute to elucidation of the mechanism of insect resistance mediated by acylsugars and help with identification of yet-unknown genes contributing to acylsugar synthesis and diversity.

Keywords

Acylsugar chemistry Insect resistance Marker-assisted selection Metabolite diversity Plant defense Secondary metabolites 

Abbreviations

ai-C5

2-Methylbutanoate (anteiso branched 5 carbon acyl group)

i-C4

2-Methylpropanoate (iso branched 4 carbon acyl group)

i-C5

3-Methylbutanoate (iso branched 5 carbon acyl group)

i-C10

8-Methylnonanoate (iso branched 10 carbon acyl group)

i-C11

9-Methyldecanoate (iso branched 11 carbon acyl group)

i-C13

11-Methyldodecanoate (iso branched 13 carbon acyl group)

n-C10

n-Decanoate (straight chain 10 carbon acyl group)

n-C12

n-Dodecanoate (straight chain 12 carbon acyl group)

Supplementary material

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11032_2016_584_MOESM2_ESM.xlsx (17 kb)
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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • John R. Smeda
    • 1
  • Anthony L. Schilmiller
    • 2
  • Robert L. Last
    • 3
    • 4
  • Martha A. Mutschler
    • 1
  1. 1.Plant Breeding and Genetics Section, School of Integrative Plant SciencesCornell UniversityIthacaUSA
  2. 2.Mass Spectrometry and Metabolomics CoreMichigan State UniversityEast LansingUSA
  3. 3.Department of Biochemistry and Molecular BiologyMichigan State UniversityEast LansingUSA
  4. 4.Department of Plant BiologyMichigan State UniversityEast LansingUSA

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