Molecular Breeding

, 36:106 | Cite as

Mapping quantitative trait loci controlling oil content, oleic acid and linoleic acid content in sunflower (Helianthus annuus L.)

  • Ameena Premnath
  • Manivannan Narayana
  • Chandirakala Ramakrishnan
  • Senthil Kuppusamy
  • Vanniarajan Chockalingam


Sunflower oil with high oleic acid content is in great demand due to its nutritional as well as industrial benefits. The trait is mainly controlled by dominant alleles at a major gene, Ol, with other modifiers. The objectives of this research were to map the oil content, oleic acid and linoleic acid content in sunflower seeds. An F2 mapping population from cytoplasmic male-sterile line COSF 7A (33–35 % oleic acid) and high oleic acid inbred line HO 5–13 (88–90 % oleic acid) was developed and phenotyped for oil content, oleic acid and linoleic acid content at the F2 seed level. High phenotypic and genotypic coefficients of variation were recorded for oleic acid and linoleic acid content. High heritability and high genetic advance as percent of mean was recorded for oleic acid and linoleic acid content. This indicated the presence of the additive type of gene action controlling the traits oleic acid content and linoleic acid content. The Ol gene was mapped to linkage group (LG) 14 and tightly linked to the marker HO_Fsp_b. In addition, two more quantitative trait loci (QTLs) for oleic acid content were identified in LG8 and LG9. Two QTLs for oil content and two QTLs for linoleic acid content were also identified. All these QTLs explained over 10 % of phenotypic variation. A study was conducted with 13 genotypes differing in oil quality as well as quantity over three seasons to assess the reliability of the identified QTLs over seasons. It resulted in the identification of two potential QTLs for oleic acid as well as linoleic acid content with the markers ORS 762 and HO_Fsp_b. These markers explained more than 57.6–66.6 % of phenotypic variation. Hence it can be concluded that these markers/QTLs would be useful in the marker-assisted selection breeding programme to improve oil quality. The present study also indicated the presence of at least two other genomic regions controlling oleic and linoleic acid content in sunflower.


Helianthus annuus Oil content Oleic acid Linoleic acid QTL 



Ameena Premnath was supported by INSPIRE fellowship (IF 120124) of Department of Science and Technology, Government of India.


  1. Al-Chaarani RG, Gentzbittel L, Huang XQ, Sarrafi A (2004) Genotypic variation and identification of QTLs for agronomic traits, using AFLP and SSR markers in RILs of sunflower (Helianthus annuus L.). Theor Appl Genet 109(7):1353–1360CrossRefPubMedGoogle Scholar
  2. Burke JM, Knapp SJ, Rieseberg LH (2005) Genetic consequences of selection during the evolution of cultivated sunflower. Genetics 171:1933–1940CrossRefPubMedPubMedCentralGoogle Scholar
  3. Canvin DT (1965) The effect of temperature on the oil content and fatty acid composition of the oils from several oilseed crops. Can J Bot 43:63–69CrossRefGoogle Scholar
  4. Codex Alimentarius Commission (2005) Twenty-eighth session report. FAO Headquarters, Rome, pp 4–9Google Scholar
  5. Delplanque B (2000) Intéret nutritionnel des tournesols. Proceedings of XV International Sunflower Conference, Toulouse 1:15–16Google Scholar
  6. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
  7. Ebrahimi A, Maury P, Berger M, Poormohammad Kiani S, Nabipour A, Shariati F, Grieu P, Sarrafi A (2008) QTL mapping of seed-quality traits in sunflower recombinant inbred lines under different water regimes. Genome 51(8):599–615CrossRefPubMedGoogle Scholar
  8. Fernandez-Martinez J, Jimenez A, Dominguez J, Garcia JM, Garces R, Mancha M (1989) Genetic analysis of the high oleic acid content in cultivated sunflower (Helianthus annuus L.). Euphytica 41:39–51CrossRefGoogle Scholar
  9. Fuller M, Diamond J, Applewhite T (1967) High oleic sunflower oil stability and chemical modification. J Am Oil Chem Soc 44:264–267CrossRefPubMedGoogle Scholar
  10. Grundy SM (1986) Comparison of monounsaturated fatty acids and carbohydrates for lowering plasma cholesterol. N Engl J Med 314:745–748CrossRefPubMedGoogle Scholar
  11. Hongtrakul V, Slabaugh MB, Knapp SJ (1998) A seed specific delta-12 oleate desaturase gene is duplicated, rearranged, and weakly expressed in high oleic acid sunflower lines. Crop Sci 38(5):1245–1249CrossRefGoogle Scholar
  12. Hu J (2006) Defining the sunflower (Helianthus annuus L.) linkage group ends with the Arabidopsis-type telomere sequence repeat-derived markers. Chromosome Res 14:535–548CrossRefPubMedGoogle Scholar
  13. Johnson HW, Robinson HF, Comstock RE (1955) Estimates of genetic and environmental variability in soybean. Agron J 47:314–318CrossRefGoogle Scholar
  14. Kinman ML, Earle FR (1964) Agronomic performance and chemical composition of the seed of sunflower hybrids and introduced varieties. Crop Sci 4:417–420CrossRefGoogle Scholar
  15. Lacombe S, Berville A (2001) A dominant mutation for high oleic acid content in sunflower (Helianthus annuus L.) seed oil is genetically linked to a single oleate-desaturase RFLP locus. Mol Breed 8(2):129–137CrossRefGoogle Scholar
  16. Lacombe S, Souyris I, Berville AJ (2009) An insertion of oleate desaturase homologous sequence silences via siRNA the functional gene leading to high oleic acid content in sunflower seed oil. Mol Genet Genom 281:43–54CrossRefGoogle Scholar
  17. Miller JF, Zimmerman DC, Vick BA (1987) Genetic control of high oleic acid content in sunflower oil. Crop Sci 27(5):923–926CrossRefGoogle Scholar
  18. Perez-Vich B, Fernandez-Martinez JM, Grondona M, Knapp SJ, Berry ST (2002) Stearoyl-ACP and oleoyl-PC desaturase genes cosegregate with quantitative trait loci underlying high stearic and high oleic acid mutant phenotypes in sunflower. Theor Appl Genet 104(2–3):338–349CrossRefPubMedGoogle Scholar
  19. Schuppert GF, Tang S, Slabaugh MB, Knapp SJ (2006) The sunflower high-oleic mutant Ol carries variable tandem repeats of FAD2-1, a seed specific oleoyl-phosphatidyl choline desaturase. Mol Breed 17:214–256CrossRefGoogle Scholar
  20. Soldatov KI (1976) Chemical mutagenesis in sunflower breeding. In: Proceedings of 7th international sunflower association, Vlaardingen, The Netherlands, pp 352–357Google Scholar
  21. Voorrips RE (2006) MapChart 2.2: software for the graphical presentation of linkage maps and QTLs. Plant Research International, WageningenGoogle Scholar
  22. Zeng ZB (1993) Theoretical basis for separation of multiple linked gene effects in mapping quantitative trait loci. In: Proceedings of the National Academy of Sciences of the USA, pp 10972–10976Google Scholar
  23. Zeng ZB (1994) Precision mapping of quantitative trait loci. Genetics 136(4):1457–1468PubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  1. 1.Department of Oilseeds, Centre for Plant Breeding and GeneticsTamil Nadu Agricultural University (TNAU)CoimbatoreIndia
  2. 2.Department of Remote Sensing and Geographic Information System, Natural Resource ManagementTamil Nadu Agricultural University (TNAU)CoimbatoreIndia
  3. 3.Department of Plant Breeding and Genetics, Agricultural College and Research InstituteTamil Nadu Agricultural University (TNAU)MaduraiIndia

Personalised recommendations