Skip to main content

A codominant diagnostic marker for the slow ripening trait in peach


The peach [Prunus persica L. (Batsch)] slow ripening (SR) trait is a mutation preventing the normal ripening process. Individuals with this phenotype are discarded in peach breeding programs. This trait is determined by a single gene (Sr/sr), where the recessive homozygote (sr/sr) confers the SR phenotype, and has been mapped to linkage group 4 of the peach genome. A large deletion of 26.6 kb containing the sequence of a NAC transcription factor has been proposed as the causal mutation. Two dominant markers based on the sequence of this region have been assayed previously and found to be diagnostic (genotypes always predicted the phenotypes). However, their dominant nature—a null allele for the marker was associated with the sr allele—made it impossible to predict the individuals that carried the SR trait. Here we used resequencing information to develop a codominant molecular marker for the SR trait in peach. The marker was validated in the ‘Belbinette’ × ‘Nectalady’ F1 and the ‘Venus’ F2 populations, and in 27 lines, 18 of which are known to carry the sr allele. The marker cosegregated with the SR phenotype in all cases, allowing the discrimination of two DNA fragments of different size associated with normal-ripening alleles, in addition to a third fragment associated with the sr allele. The utility of this marker in peach breeding programs is discussed.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2


  • Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15

    Google Scholar 

  • Eduardo I, Picañol R, Rojas E, Batlle I, Howad W, Aranzana MJ, Arús P (2015) Mapping of a major gene for the slow ripening character in peach: co-location with the maturity date gene and development of a candidate gene-based diagnostic marker for its selection. Euphytica 205:627–636

    Article  CAS  Google Scholar 

  • Martínez-García PJ, Parfitt DE, Ogundiwin EA, Fass J, Chan HM, Ahmad R, Lurie S, Dandekar A, Gradziel TM, Crisosto CH (2013) High density SNP mapping and QTL analysis for fruit quality characteristics in peach (Prunus persica L.). Tree Genet Genomes 9:19–36

    Article  Google Scholar 

  • Nuñez-Lillo G, Cifuentes-Esquivel A, Troggio M, Micheletti D, Infante R, Campos-Vargas R, Orellana A, Blanco-Herrera F, Meneses C (2015) Identification of candidate genes associated with mealiness and maturity date in peach [Prunus persica (L.) Batsch] using QTL analysis and deep sequencing. Tree Genet Genomes 11:86

    Article  Google Scholar 

  • Picañol R, Eduardo I, Aranzana MJ, Howad W, Batlle I, Iglesias I, Alonso JM, Arús P (2013) Combining linkage and association mapping to search for markers linked to the flat fruit character in peach. Euphytica 190:279–288

    Article  Google Scholar 

  • Pirona R, Eduardo I, Pacheco I, Da Silva LingeC, Miculan M, Verde I, Tartarini S, Dondini L, Pea G, Bassi D, Rossini L (2013) Fine mapping and identification of a candidate gene for a major locus controlling maturity date in peach. BMC Plant Biol 13:166

    Article  PubMed  PubMed Central  Google Scholar 

  • Ramming DW (1991) Genetic control of a slow-ripening fruit trait in nectarine. Can J Plant Sci 71:601–603

    Article  Google Scholar 

  • Ru S, Main D, Evans K, Peace C (2015) Current applications, challenges, and perspectives of marker-assisted seedling selection in Rosaceae tree fruit breeding. Tree Genet Genomes 11:8

    Article  Google Scholar 

Download references


Funds for this research were provided in part by a project of the Spanish Ministry of Economy and Knowledge (AGL2012-40228-C02-01). This work also was supported by Fondo de Areas Prioritarias Centro de Regulación del Genoma 15090007, CORFO Consorcio Biofrutales 13 CTI-21520-SP03 and 13 CTI-21520-SP04, FONDECYT 1160584, FONDEF Genoma G13i10005 and CORFO-Innova 09PMG7240.

Author information

Authors and Affiliations


Corresponding author

Correspondence to Iban Eduardo.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Meneses, C., Ulloa-Zepeda, L., Cifuentes-Esquivel, A. et al. A codominant diagnostic marker for the slow ripening trait in peach. Mol Breeding 36, 77 (2016).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI:


  • Fruit quality
  • Molecular marker
  • Prunus persica
  • Marker-assisted selection