Molecular Breeding

, 36:41 | Cite as

Construction of a SNP and SSR linkage map in autotetraploid blueberry using genotyping by sequencing

  • Susan McCallum
  • Julie Graham
  • Linzi Jorgensen
  • Lisa J. Rowland
  • Nahla V. Bassil
  • James F. Hancock
  • Edmund J. Wheeler
  • Kelly Vining
  • Jesse A. Poland
  • James W. Olmstead
  • Emily Buck
  • Claudia Wiedow
  • Eric Jackson
  • Allan Brown
  • Christine A. Hackett


The construction of the first genetic map in autotetraploid blueberry has been made possible by the development of new SNP markers developed using genotyping by sequencing in a mapping population created from a cross between two key highbush blueberry cultivars, Draper × Jewel (Vaccinium corymbosum). The novel SNP markers were supplemented with existing SSR markers to enable the alignment of parental maps.  In total, 1794 single nucleotide polymorphic (SNP) markers and 233 simple sequence repeat (SSR) markers exhibited segregation patterns consistent with a random chromosomal segregation model for meiosis in an autotetraploid. Of these, 700 SNPs and 85 SSRs were utilized for construction of the ‘Draper’ genetic map, and 450 SNPs and 86 SSRs for the ‘Jewel’ map.  The ‘Draper’ map comprises 12  linkage groups (LG), associated with the haploid chromosome number for blueberry, and totals 1621 cM while the ‘Jewel’ map comprises 20 linkage groups totalling 1610 cM. Tentative alignments of the two parental maps have been made on the basis of shared SSR alleles and linkages to double-simplex markers segregating in both parents. Tentative alignments of the two parental maps have been made on the basis of shared SSR alleles and linkages to double-simplex markers segregating in both parents.


Vaccinium corymbosum Autotetraploid TetraploidMap Genotyping by sequencing 



This work was funded through Horticulture Link (HL0190), and all contributing partners are gratefully acknowledged. Project collaboration with the Specialty Crop Research Initiative-funded project (Grant 2008-51180-04861 entitled ‘Generating genomic tools for blueberry improvement’) has provided valuable access to plant material, genetic resources and advice. Support for this work from the Scottish Government’s Rural and Environment Science and Analytical Services Division (RESAS) is gratefully acknowledged. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the James Hutton Institute or any of the other agencies involved in this research. We thank all reviewers for their comments and Sue Gardiner for assisting in preparation of this ms.

Supplementary material

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Supplementary material 1 (DOCX 20 kb)
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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Susan McCallum
    • 1
  • Julie Graham
    • 1
  • Linzi Jorgensen
    • 1
  • Lisa J. Rowland
    • 2
  • Nahla V. Bassil
    • 3
  • James F. Hancock
    • 4
  • Edmund J. Wheeler
    • 5
  • Kelly Vining
    • 6
  • Jesse A. Poland
    • 7
  • James W. Olmstead
    • 8
  • Emily Buck
    • 9
  • Claudia Wiedow
    • 9
  • Eric Jackson
    • 10
  • Allan Brown
    • 11
  • Christine A. Hackett
    • 12
  1. 1.Cell and Molecular Science ProgrammeJames Hutton InstituteDundeeScotland, UK
  2. 2.Genetic Improvement of Fruits and Vegetables LaboratoryUSDA-ARSBeltsvilleUSA
  3. 3.National Clonal Germplasm RepositoryUSDA-ARSCorvallisUSA
  4. 4.Department of HorticultureMichigan State UniversityEast LansingUSA
  5. 5.MBG MarketingBerry BlueGrand JunctionUSA
  6. 6.Department of HorticultureOregon State UniversityCorvallisUSA
  7. 7.Wheat Genetics Resource Center, Department of Plant Pathology and Department of AgronomyKansas State UniversityManhattanUSA
  8. 8.Horticultural Sciences DepartmentUniversity of FloridaGainesvilleUSA
  9. 9.The New Zealand Institute for Plant and Food Research LimitedPalmerston NorthNew Zealand
  10. 10.General Mills Crop BiosciencesWheat Innovation CenterManhattanUSA
  11. 11.Plants for Human Health Institute, Department of Horticultural ScienceNorth Carolina State UniversityKannapolisUSA
  12. 12.Biomathematics and Statistics ScotlandDundeeScotland, UK

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