Abstract
Natural genetic variation exists in animals and plants. Mining and utilizing this variation may provide benefits for new breed/cultivar development. From screening over 4000 cultivated peanut germplasm accessions, we identified two natural mutant lines (PI 342664 and PI 342666) with 80 % oleic acid by gas chromatography analysis. It is known that FAD2A and FAD2B are the two major genes involved in the conversion of oleic to linoleic acid in peanuts by fatty acid desaturase. Functional mutations in one or both genes can alter the oleate level. By sequencing the coding region of these two genes, we identified a substitution of G448A in FAD2A and a substitution of C301G in FAD2B for both mutant lines. The substitution in FAD2A is the same as a previously identified one, resulting in a missense amino acid substitution of D150N, but the substitution in FAD2B is a new one, resulting in H101D. The new amino acid substitution on FAD2B is located in the first histidine box (one of the active sites) of the fatty acid desaturase and may significantly decrease its activity. Our mutants did not have flowers on the main stem (subspecies hypogaea), but F435 (a previously identified natural high-oleate mutant) had flowers on the main stem (subspecies fastigiata). Therefore, we identified a class of natural mutants from the subspecies hypogaea and provided new additional genetic resources for breeders to use. Our results also demonstrate a good example of the importance and usefulness for preserving natural genetic diversity and utilizing plant germplasm collections.
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References
Adehubei IA, Schmidt S, Peshkin L, Ramensky VE, Geramosiva A, Bork P, Kondrashov AS, Sunyaev SR (2010) A method and server for predicting damaging missense mutations. Nat Methods 7:248–249
Ahmed EM, Young CT (1982) Composition, quality, and flavor of peanuts. In: Pattee HE, Young CT (eds) Peanut science and technology. American Peanut Research and Education Society, Inc., Yoakum, pp 655–688
Barkley NA, Chenault-Chamberlin KD, Wang ML, Pittman RN (2010) Development of a real- time PCR genotyping assay to identify high oleic acid peanuts (Arachis hypogaea L.). Mol Breed 25:541–548
Barkley NA, Wang ML, Pittman RN (2011) A real-time PCR genotyping assay to detect FAD2A SNPs in peanuts (Arachis hypogaea L.). Electron J Biotechnol. doi:10.2225/vol14issue1-fulltext-12
Braddock JC, Simis CA, O’keefe SF (1995) Flavor and oxidative stability of toasted high oleic acid peanuts. J Food Sci 60:489–493
Bruner AC, Jung S, Abbort AG, Powell GL (2001) The naturally occurring high oleate oil character in some peanut varieties results from reduced oleoyl-PC desaturase activity from mutation of aspartate 150 to asparagines. Crop Sci 41:522–526
Chamberlin KD, Bennett RS, Damicone JP, Godsey CB, Melouk HA, Keim K (2015) Registration of ‘OLé’ peanut. J Plant Regist 9:154–158
Chen Z, Wang ML, Barkley NA, Pittman RN (2010) A simple allele-specific PCR assay for detection FAD2 alleles in both the A and B genome of cultivated peanut for high oleate trait selection. Plant Mol Rep 28:542–548
Chen CY, Barkley NA, Wang ML, Holbrook CC, Dang PM (2014) Registration of purified accessions for the US peanut mini-core germplasm collection. J Plant Regist 8:77–85
Chu Y, Ramos L, Holbrook CC, Ozias-Akins P (2007) Frequency of a loss-of-function mutation in oleo-PC desaturase (ahFAD2A) in the mini-core of the US peanut germplasm collection. Crop Sci 47:2372–2378
Chu Y, Ramos L, Holbrook CC, Ozias-Akins P (2009) Two alleles of ahFAD2B control the high oleic acid trait in cultivated peanut. Crop Sci 49:2029–2036
Derbyshire EJ (2014) A review of the nutritional composition, organoleptic characteristics and biological effects of the high oleic peanut. Int J Food Sci Nutr 65:781–790
Fang CQ, Wang CT, Wang PW, Tang YY, Wang XZ, Cui FG, Yu SL (2012) identification of a novel mutation in FAD2B from a peanut EMS mutant with elevated oleate content. J Oleo Sci 61:143–148
Food and Agriculture Organization (FAO, 2015) Food and Agricultural Organization of the United Nations, FAO statistical database. In http://www.fao.org/. Accessed on 18th May 2015
Gorbet DW, Knauft DA (2000) Registration of ‘SunOleic 97R’ peanut. Crop Sci 40:1190–1191
Gorbet DW, Tillman BL (2009) Registration of ‘Florida-07’ peanut. J Plant Regist 3:14–18
Jung S, Swift D, Sengoku E, Patel M, Teule F, Powell G, Moore K, Abbot A (2000a) The high oleate trait in the cultivated peanut (Arachis hypogaea L.)—I: isolation and characterization of two genes encoding microsomal oleoyl-PC desaturases. Mol Gen Genet 263:796–805
Jung S, Powell G, Moore K, Abbott A (2000b) The high oleate trait in the cultivated peanut (Arachis hypogaea L.)—II: molecular basis and genetics of the trait. Mol Gen Genet 263:806–811
Krapovickas A, Gregory WC (1994) Taxonomia del énero Arachis (Leguminosae). Bonplandia 8:1–186
Kumar P, Henikoff S, Ng PC (2009) Predicting the effects of coding non-synonymous variants on protein function using the SIFT algorithm. Nat Protoc 4:1073–1081
Lópes Y, Nadaf HL, Smith OD, Connell JP, Reddy AS, Fritz AK (2000) Isolation and characterization of the Delta (12)-fatty acid desaturase in peanut (Arachis hypogaea L.) and search for polymorphisms for the high oleate trait in Spanish market-type lines. Theor Appl Genet 101:1131–1138
Norden AJ, Borget DW, Knauft DA, Young CT (1987) Variability in oil quality among peanut genotypes in the Florida breeding program. Peanut Sci 4:7–11
Patel M, Jung S, Moore K, Powell G, Ainsworth C, Abbot A (2004) High-oleate peanut mutants result from a mite insertion into the FAD2 gene. Theor Appl Genet 108:1492–1502
Vassiliou EK, Gonzalez A, Garcia C, Tadro JH, Chakraborty G, Toney JH (2009) Oleic acid and peanut oil high in oleic acid reverse the inhibitory effect of insulin production of the inflammatory cytokine TNF-α both in vitro and in vivo system. Lipid Health Dis 8:25
Wang ML, Chen CY, Davis J, Guo B, Stalker HT, Pittman RN (2009) Assessment of oil content and fatty acid composition variability in different peanut subspecies and botanical varieties. Plant Genet Resour 8:71–73
Wang CT, Tang YY, Wang XZ, Zhang SW, Li GJ, Zhang JC, Yu SY (2011) Sodium azide mutagenesis resulted in a peanut plant with elevated oleate content. Electron J Biotechnol. doi:10.110/vol14-issue2-fulltext-4
Wang ML, Chen CY, Tonnis B, Barkley NA, Pinnow DL, Pittman RN, Davis J, Holbrook CC, Stalker HT, Pederson GA (2013) Oil, fatty acid, flavonoid, and resveratrol content variability and FAD2A functional SNP genotypes in the US peanut mini-core collection. J Agric Food Chem 61:2875–2882
Acknowledgments
The authors gratefully thank Ms. Phiffie Vankus and Ms. Angie Lewis for their excellent assistance on seed germination and greenhouse management, Mr. Jerry Davis and Mr. Rick Meyer for their help on statistical and bioinformatic analysis, and Drs. Zhenbang Chen and Paul Raymer for useful suggestions on improving the quality of this manuscript.
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Wang, M.L., Tonnis, B., An, YQ.C. et al. Newly identified natural high-oleate mutant from Arachis hypogaea L. subsp. hypogaea . Mol Breeding 35, 186 (2015). https://doi.org/10.1007/s11032-015-0377-3
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DOI: https://doi.org/10.1007/s11032-015-0377-3