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Molecular Breeding

, 35:135 | Cite as

QTLs detected for individual sugars and soluble solids content in apple

  • Yingzhu Guan
  • Cameron Peace
  • David Rudell
  • Sujeet Verma
  • Kate EvansEmail author
Article

Abstract

Sweetness is one of the most important fruit quality traits in breeding programs, determining the overall quality and flavor perception of apples. Selecting for this trait using conventional breeding methods is challenging due to the complexity of its genetic control. In order to improve the efficiency of trait selection via DNA-based markers, extensive studies focused on the detection of quantitative trait loci (QTL) and the development of DNA-based markers associated with QTL regions for traits of interest. Newly discovered QTLs detected in multiple apple breeding populations are presented here for individual sugars (fructose, glucose, sucrose, and sorbitol) and soluble solids content (SSC) at harvest, after 10, and 20 weeks of refrigerated storage followed by 1 week at room temperature in two successive years. A total of 1416 polymorphic SNPs were filtered from the RosBreed Apple SNP Infinium® array for QTL analysis using FlexQTL™ software. QTLs for individual sugars were identified on linkage groups (LG) 1, 2, 3, 4, 5, 9, 11, 12, 13, 15, and 16, and QTLs for SSC were found on LGs 2, 3, 12, 13, and 15. One QTL region on LG 1 was consistently identified for both fructose and sucrose from harvest through storage in both years, which accounted for 34–67 and 13–41 % of total phenotypic variation, respectively. These stable QTLs with high explained phenotypic variation on LG 1 for fructose content indicate a promising genomic region for DNA-based marker development to enable marker-assisted breeding for sweetness selection in apple breeding programs.

Keywords

Fruit quality Marker-assisted breeding RosBREED SNP Soluble solids content 

Notes

Acknowledgments

This work was partially funded by USDA’s National Institute of Food and Agriculture—Specialty Crop Research Initiative project, ‘RosBREED: enabling marker-assisted breeding in Rosaceae’ (2009-51181-05808). We thank Nancy Buchanan, Julia Harshman, Lisa Brutcher, and Bonnie Konishi for assistance in phenotyping, Dave Buchanan for his skillful experience on GC–MS, Jerry Tangren for his assistance with statistical analysis of phenotypic data, Umesh Rosyara for his assistance in QTL analysis, Marco Bink and Eric van de Weg for training in Pedigree-Based Analysis concepts and software.

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Yingzhu Guan
    • 1
  • Cameron Peace
    • 2
  • David Rudell
    • 3
  • Sujeet Verma
    • 2
  • Kate Evans
    • 1
    Email author
  1. 1.Department of Horticulture, Tree Fruit Research and Extension CenterWashington State UniversityWenatcheeUSA
  2. 2.Department of HorticultureWashington State UniversityPullmanUSA
  3. 3.Tree Fruit Research LaboratoryUSDA-ARSWenatcheeUSA

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