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Genome-wide scans reveal genetic architecture of apple flavour volatiles

Molecular Breeding volume 35, Article number: 118 (2015) Cite this article

Abstract

Flavour is an important food trait, yet little is known about the genetic architecture and mode of inheritance of apple flavour compounds. The objectives of this study were to: understand the inheritance of flavour volatiles in a clonally replicated germplasm population; unravel correlation networks of volatiles; and to use genome-wide single nucleotide polymorphism (SNP) markers to identify genomic regions that play a role in the expression of flavour volatiles. This analysis revealed that more than half of the 37 volatiles (measured by gas chromatography–mass spectrometry) showed high heritability (h 2 > 0.4), with only a small number (3 of the 37) displaying low heritability (h 2 < 0.2). Majority (~85 %) of the significant SNP loci displayed the additive mode of inheritance. Our results supported the roles of MdAAT, MdCXE and MdLOX genes in the expression of apple flavour volatiles. Effect sizes of SNP loci, some of which are associated with multiple compounds, were small (<10 %), which is consistent with a polygenic quantitative inheritance model. New genomic locations associated with multiple flavour compounds were found, and some SNPs were associated with both sensory flavour and some flavour volatiles. Simultaneous genome-wide association study for sensory flavour and flavour compounds is recommended for unravelling genetic mechanisms to facilitate marker-assisted breeding for targeted flavours.

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Acknowledgments

The PFR Markers and Mapping Team is thanked for help in collecting leaf samples. Slipstream Automation Ltd, New Zealand, is thanked for providing high-throughput DNA extraction services. We also thank AgResearch Invermay, New Zealand, for providing Illumina® SNP array genotyping. This research was partly supported by the New Zealand Ministry of Business, Innovation and Employment (MBIE). PFR colleagues Richard Volz and Nigel Perry are thanked for their constructive feedback.

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Authors and Affiliations

  1. The New Zealand Institute for Plant and Food Research Limited (PFR), Hawke’s Bay Research Centre, Havelock North, New Zealand

    Satish Kumar & Claire Whitworth

  2. PFR, Palmerston North Research Centre, Palmerston North, New Zealand

    Daryl Rowan, Martin Hunt & David Chagné

  3. PFR, Mount Albert Research Centre, Auckland, New Zealand

    Edwige Souleyre

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  1. Satish Kumar
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  2. Daryl Rowan
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  3. Martin Hunt
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  4. David Chagné
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  5. Claire Whitworth
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  6. Edwige Souleyre
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Correspondence to Satish Kumar.

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Kumar, S., Rowan, D., Hunt, M. et al. Genome-wide scans reveal genetic architecture of apple flavour volatiles. Mol Breeding 35, 118 (2015). https://doi.org/10.1007/s11032-015-0312-7

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