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Evaluation of marker-assisted selection for the stripe rust resistance gene Yr15, introgressed from wild emmer wheat

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Abstract

Stripe rust disease is caused by the fungus Puccinia striiformis f. sp. tritici and severely threatens wheat worldwide, repeatedly breaking resistance conferred by resistance genes and evolving more aggressive strains. Wild emmer wheat, Triticum dicoccoides, is an important source for novel stripe rust resistance (Yr) genes. Yr15, a major gene located on chromosome 1BS of T. dicoccoides, was previously reported to confer resistance to a broad spectrum of stripe rust isolates, at both seedling and adult plant stages. Introgressions of Yr15 into cultivated T. aestivum bread wheat and T. durum pasta wheat that began in the 1980s are widely used. In the present study, we aimed to validate SSR markers from the Yr15 region as efficient tools for marker-assisted selection (MAS) for introgression of Yr15 into wheat and to compare the outcome of gene introgression by MAS and by conventional phenotypic selection. Our findings establish the validity of MAS for introgression of Yr15 into wheat. We show that the size of the introgressed segment, defined by flanking markers, varies for both phenotypic selection and MAS. The genetic distance of the MAS marker from Yr15 and the number of backcross steps were the main factors affecting the length of the introgressed donor segments. Markers Xbarc8 and Xgwm493, which are the nearest flanking markers studied, were consistent and polymorphic in all 34 introgressions reported here and are therefore the most recommended markers for the introgression of Yr15 into wheat cultivars. Introgression directed by markers, rather than by phenotype, will facilitate simultaneous selection for multiple stripe rust resistant genes and will help to avoid escapees during the selection process.

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Acknowledgments

This work was supported by grants from Sixth Framework Programme (FP6) of the European Union through the BioExploit project (CT-2005-513949), the United States—Israel Binational Agricultural Research and Development Fund (BARD), Grant IS-4628-13, and the Israel Science Foundation equipment Grant 1719/08. Dina Raats is grateful for the Eshkol Fellowship awarded by the Israeli Ministry of Science. Dr. J. Dubcovsky acknowledges support from HHMI, GBMF, and USDA-NIFA Grant 2011-68002-30029. The authors would like to acknowledge the help of the late Dr. Z. Gerechter-Amitai from the Agricultural Research Organization, Beit Dagan, Israel, Dr. X. Zhang from the University of California, and Drs. R. McIntosh and T. The from the University of Sydney, Australia, in providing us with the Yr15 introgression lines and the corresponding recipient lines, together with their pedigree information. The authors thank Dr. J. Manisterski and P. Ben-Yehuda from Tel Aviv University for providing spores of Puccinia striiformis isolate 5006 (race 38E134). The authors would like to thank O. Gurevich and T. Kis-Papo from the University of Haifa, Israel for their skillful technical assistance.

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  1. Elitsur Yaniv

    Present address: LUKE/BI Plant Genomics Lab, Institute of Biotechnology, Viikki Biocenter, University of Helsinki, P.O. Box 65, Helsinki, Finland

Authors and Affiliations

  1. Plant Genomics and Disease Resistance Laboratory, Department of Evolutionary and Environmental Biology, Institute of Evolution, Faculty of Natural Sciences, University of Haifa, Haifa, Israel

    Elitsur Yaniv, Dina Raats, Yefim Ronin, Abraham B. Korol & Tzion Fahima

  2. Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel

    Adriana Grama

  3. Department of Plant and Food Sciences, University of Sydney, Sydney, Australia

    Harbans Bariana

  4. Department of Plant Sciences, University of California, Davis, CA, USA

    Jorge Dubcovsky

  5. Howard Hughes Medical Institute, Chevy Chase, MD, 20815-6789, USA

    Jorge Dubcovsky

  6. LUKE/BI Plant Genomics Lab, Institute of Biotechnology, Viikki Biocenter, University of Helsinki, P.O. Box 65, Helsinki, Finland

    Alan H. Schulman

  7. LUKE Natural Resources Institute Finland, Jokioinen, Finland

    Alan H. Schulman

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  1. Elitsur Yaniv
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Correspondence to Tzion Fahima.

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Elitsur Yaniv and Dina Raats have contributed equally to this work.

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Yaniv, E., Raats, D., Ronin, Y. et al. Evaluation of marker-assisted selection for the stripe rust resistance gene Yr15, introgressed from wild emmer wheat. Mol Breeding 35, 43 (2015). https://doi.org/10.1007/s11032-015-0238-0

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