Skip to main content

Advertisement

SpringerLink
Log in

Quantitative trait loci regulating the fatty acid profile of acylsugars in tomato

  • Published:
Molecular Breeding Aims and scope Submit manuscript

Abstract

Acylsugars are secondary metabolites with proven insect resistance properties that are produced by many Solanaceous species including Solanum pennellii, a wild relative of tomato. The acylsugar chemotypes of S. pennellii varies greatly within its natural range and might be the product of plant/insect coevolution. The S. pennellii accession LA716 was used to transfer increased levels of acylsugar production into the cultivated tomato, resulting in the acylsugar tomato breeding line CU071026. S. pennellii accession LA716 produces high levels of acylsugars with chemotypes that differ greatly from those produced by CU071026 or the trace acylsugars of cultivated tomato. Understanding the genetic regulation of acylsugar chemistry will aid efforts to breed acylsugar production into cultivated tomato, allowing for alteration of both acylsugar base moieties and fatty acid profiles. This study uses a BC1F1 population produced from the cross of S. pennellii LA716 and CU071026 with CU071026 as the recurrent parent to identify QTL that change the fatty acid profile of acylsugars. Multiple QTL and epistatic interactions between QTL were detected including three QTL on chromosomes 2, 5, and 7, which significantly alter the percentage of extended iso-odd branched fatty acids and straight chain fatty acids on the acylsugars. We also report the introgression of one of these QTL, FA 2, into CU071026, resulting in a new tomato line with significantly increased i11:0 as a percentage of the fatty acids in its acylsugars. Candidate genes for these QTL and the impacts of altering acylsugar fatty acid are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Blauth SL, Steffens JC, Churchill GA, Mutschler MA (1999) QTL analysis of acylsugar fatty acid constitutents using intraspecific populations of the wild tomato Lycopersicon pennellii. Theor Appl Genet 99:373–381

    Article  Google Scholar 

  • Burke B, Goldsby G, Mudd JB (1987) Polar epicuticular lipids of Lycopersicon pennellii. Phytochemistry 26:2567–2571

    Article  CAS  Google Scholar 

  • Chortek OT, Pomonis JG, Johnson AW (1996) Syntheses and characterizations of insecticidal sucrose esters. J Agric Food Chem 44:1551–1557

    Article  Google Scholar 

  • Churchhill GA, Doerge RW (1994) Empirical threshold for quantitative trait mapping. Genetics 138:963–971

    Google Scholar 

  • D’Auria JC (2006) Acyltransferases in plants: a good time to be BAHD. Curr Opin Plant Biol 9:331–340

    Article  PubMed  Google Scholar 

  • Eshed Y, Zamir D (1994) A genomic library of Lycopersicon pennellii in L. esculentum: a tool for fine mapping of genes. Euphytica 79:175–179

    Article  CAS  Google Scholar 

  • Fobes JF, Mudd J, Marsden M (1985) Epicuticular lipid on the leaves of L. pennellii and L. esculentum. Plant Physiol 77:567–570

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Ghangas GS, Steffens JC (1993) UDPglucose: fatty acid transglucosylation and transacylation in triacylglucose biosynthesis. Proc Natl Acad Sci USA 90:9911–9915

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Goffreda JC, Mutschler MA (1989) Inheritance of potato aphid resistance in hybrids between Lycopersicon esculentum and L. pennellii. Theor Appl Genet 78:210–216

    Article  PubMed  CAS  Google Scholar 

  • Goffreda JC, Mutschler MA, Steffens JC (1990) Association of epicuticular sugars with aphid resistance in hybrids with wild tomato. J Am Soc Hortic Sci 117:161–164

    Google Scholar 

  • Hawthorne DM, Shapiro JA, Tingey WM, Mutschler MA (1992) Trichome-borne and artificially applied acylsugars of wild tomato deter feeding and oviposition of the leafminer, Liriomyza trifolii. Entomol Exp Appl 65:65–73

    Article  CAS  Google Scholar 

  • Juvik J, Shapiro JA, Young TE, Mutschler MA (1994) Acylglucoses of the wild tomato Lycopersicon pennellii alter behavior and reduce growth and survival of Helicoverpa zea and Spodoptera exigua. J Econ Entomol 87:482–492

    CAS  Google Scholar 

  • Kuai JP, Ghangas GS, Steffens JC (1997) Regulation of triacylglucose fatty acid composition—uridine diphosphate glucose fatty acid glucosyltransferases with overlapping chain-length specificity. Plant Physiol 115:1581–1587

    PubMed  CAS  PubMed Central  Google Scholar 

  • Leckie BM, DeJong DM, Mutschler MA (2012) Quantitative trait loci increasing acylsugars in tomato breeding lines and their impacts on silverleaf whiteflies. Mol Breed 31:957–970

    Article  Google Scholar 

  • Leckie BM, DeJong DM, Mutschler MA (2013) Quantitative trait loci regulating sugar moiety of acylsugars in tomato. Mol Breed 31:957–970

    Article  CAS  Google Scholar 

  • Li AX, Steffens JC (2000) An acyltransferase catalyzing the formation of diacylglucose is a serine carboxypeptidase-like protein. Proc Natl Acad Sci USA 97:6902–6907

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Li AX, Eannetta N, Ghangas GS, Steffens JC (1999) Glucose polyester biosynthesis. Purification and characterization of a glucose acyltransferase. Plant Physiol 121:453–460

    Article  PubMed  PubMed Central  Google Scholar 

  • Liedl BE, Lawson DM, White KK, Shapiro JA, Cohen DE, Carson WG, Trumble JT, Mutschler MA (1995) Acylglucoses of the wild tomato Lycopersicon pennellii alters settling and reduces oviposition of Bemisia argentifolii. J Econ Entomol 88:742–748

    CAS  Google Scholar 

  • Liu TX, Stansly PA, Chortyk OT (1996) Insecticidal activity of natural and synthetic sugar esters against Bemisia argentifolii. J Econ Entomol 89:1233–1239

    CAS  Google Scholar 

  • Manly KF, Cudmore RH Jr, Meer JM (2001) Map manager QTX, cross-platform software for genetic mapping. Mamm Genome 12:930–932

    Article  PubMed  CAS  Google Scholar 

  • Mazourek M, Pujar A, Borovsky Y, Paran I, Mueller L, Jahn MM (2009) A dynamic interface for capsaicinoid systems biology. Plant Physiol 150:1806–1821

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • McKenzie CL, Puterka GJ (2004) Effect of sucrose octanoate on survival of nymphal and adult Diaphorina citri (Homoptera: Psyllidae). J Econ Entomol 97:970–975

    Article  PubMed  CAS  Google Scholar 

  • Mutschler MA, Wintermantel WM (2006) Reducing virus associated crop loss through resistance to insect vectors. In: Loebenstein G, Carr JP (eds) natural resistance mechanisms of plants to viruses. Springer, Dordrecht, pp 241–260

    Chapter  Google Scholar 

  • Puterka GJ, Farone W, Palmer T, Barrington A (2003) Structure-function relationships affecting the insecticidal and miticidal activity of sugar esters. J Econ Entomol 96:636–644

    Article  PubMed  CAS  Google Scholar 

  • Rodriguez AE, Tingey WM, Mutschler MA (1993) Acylsugars produced by type IV trichomes of Lycopersicon pennellii (Corr.)D’Arcy deter settling of the green peach aphid, Myzus persicae (Sulzer) (Homoptera: Aphididae). J Econ Entomol 86:34–39

    CAS  Google Scholar 

  • SAS Institute Inc. (2010) JMP 9 user guide. SAS Institute Inc., Cary, NC

    Google Scholar 

  • Schilmiller A, Shi F, Kim J, Charbonneau A, Holmes D, Jones AD, Last RL (2010) Mass spectrometry screening reveals widespread diversity in trichome specialized metabolites of tomato chromosomal substitution lines. Plant J 62:391–403

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Schilmiller AL, Charbonneau AL, Last RL (2012) Identification of a BAHD acetyltransferase that produces protective acyl sugars in tomato trichomes. Proc Natl Acad Sci USA 109:16377–16382

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Shapiro J, Steffens J, Mutschler MA (1994) Acylsugars of the wild tomato Lycopersicon pennellii in relation to its geographic distribution. Biochem Syst Ecol 22:545–561

    Article  CAS  Google Scholar 

  • Slocombe SP, Schauvinhold I, McQuinn RP, Besser K, Welsby NA, Harper A, Aziz N, Li Y, Larson TR, Giovannoni J, Dixon RA, Broun P (2008) Transcriptomic and reverse genetic analyses of branched-chain fatty acid and acyl sugar production in Solanum pennellii and Nicotiana benthamiana. Plant Physiol 148:1830–1846

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Stanghellini MS, Raybold P (2004) Evaluation of selected biopesticides for the late fall control of varroa mites in a northern temperate climate. Am Bee J 144:475–480

    Google Scholar 

  • Stellari GM, Mazourek M, Jahn MM (2010) Contrasting modes for loss of pungency between cultivated and wild species of Capsicum. Heredity 104:460–471

    Article  PubMed  CAS  Google Scholar 

  • Stewart C Jr, Kang BC, Liu K, Moore SL, Yoo EY, Kim BD, Paran I, Mazourek M, Jahn MM (2005) The Pun1gene for pungency in pepper encodes a putative acyltransferase. Plant J 42:675–688

    Article  PubMed  CAS  Google Scholar 

  • Stewart C Jr, Mazourek M, Stellari GM, O’Connell M, Jahn M (2007) Genetic control of pungency in C. chinense via the Pun1 locus. J Exp Bot 58:979–991

    Article  PubMed  CAS  Google Scholar 

  • van der Hoeven RS, Steffens JC (2000) Biosynthesis and elongation of short- and medium-chain-length fatty acids. Plant Physiol 122:275–282

    Article  PubMed  PubMed Central  Google Scholar 

  • Walters DS, Steffens JC (1990) Branched-chain amino acid metabolism in the biosynthesis of Lycoperiscon pennellii glucose esters. Plant Physiol 93:1544–1551

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Wu F, Eannetta NT, Xu Y, Durrett R, Mazourek M, Jahn MM, Tanksley SD (2009) A COSII genetic map of the pepper genome provides a detailed picture of synteny with tomato and new insights into recent chromosome evolution in the genus Capsicum. Theor Appl Genet 118:1279–1293

    Article  PubMed  CAS  Google Scholar 

  • Yang J, Hu C, Hu H, Yu R, Xia Z, Ye X, Zhu J (2008) QTLNetwork: mapping and visualizing genetic architecture of complex traits in experimental populations. Bioinformatics 24:721–723

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Plant Breeding and Genetics, Cornell University, 301 Bradfield Hall, Ithaca, NY, 14853, USA

    Brian M. Leckie, Darlene M. De Jong, John R. Smeda & Martha A. Mutschler

  2. Department of Ecology and Evolutionary Biology, Cornell University, 445 Corson Hall, Ithaca, NY, 14853, USA

    Rayko Halitschke & André Kessler

Authors
  1. Brian M. Leckie
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rayko Halitschke
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Darlene M. De Jong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. John R. Smeda
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. André Kessler
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Martha A. Mutschler
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Martha A. Mutschler.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 16 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Leckie, B.M., Halitschke, R., De Jong, D.M. et al. Quantitative trait loci regulating the fatty acid profile of acylsugars in tomato. Mol Breeding 34, 1201–1213 (2014). https://doi.org/10.1007/s11032-014-0110-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11032-014-0110-7

Keywords

Search

Navigation